浙江农业学报

• 园艺科学 • 上一篇    下一篇

外源喷施不同浓度γ\|氨基丁酸对韭菜生长及氮代谢的影响

  

  1. (河北农业大学 园艺学院,河北 保定 071001)
  • 出版日期:2015-09-25 发布日期:2015-09-23

Effect of spraying different concentrations of exogenous γ\|aminobutyric acid on growth and nitrogen metabolism of leek#br#

  1. (College of Horticulture, Agricultural University of Hebei, Baoding 071001, China)
  • Online:2015-09-25 Published:2015-09-23

摘要: 以基质培韭菜为试验材料,研究高氮条件下叶面喷施不同浓度γ\|氨基丁酸(GABA)对韭菜生长和无机氮代谢的影响。结果表明:GABA(25~100 mmol·L-1)处理后6 d,韭菜的株高、茎粗、叶宽、鲜质量和干质量均高于对照,其中75 mmol·L-1 GABA处理效果最显著。与对照相比,喷施25~75 mmol·L-1 GABA后0~12 d,韭菜叶片的硝态氮、亚硝态氮含量降低,而铵态氮含量和硝酸还原酶(NR)、亚硝酸还原酶(NiR)、谷氨酸脱羧酶活性(GAD)升高,其中75 mmol·L-1 GABA喷施的效果好于其他浓度处理,喷施处理时间以第6天效果最明显。说明叶面喷施GABA后可被韭菜叶面吸收,通过诱导NR,NiR等无机氮代谢关键酶的活性降低韭菜体内硝酸盐含量,提高韭菜的产量和品质。

关键词: GABA, 韭菜, 生长, 硝酸盐

Abstract:  Taking the medium culture leek as experimental material, we studied the effect of spraying different concentrations of gamma aminobutyric acid (GABA) on growth and inorganic nitrogen metabolism of leek under the condition of high nitrogen. The results showed that, the stem height, stem diameter, leaf width, fresh and dry weight of leek were higher in the treatments of 25-100 mmol·L-1 GABA than the control after 6 d, in which 75 mmol·L-1 GABA treatment was the most effective. Compared with the control, the content of nitrate nitrogen and nitrite nitrogen reduced in the treatments of 25-75 mmol·L-1 GABA during 0-12 days after spraying, while the ammonium nitrogen content and the activity of nitrate reductase (NR), nitrite reductase (NiR), glutamic decarboxylase (GAD) increased. The effect of 75 mmol·L-1 GABA treatment was better than the other treatments, which got the most obvious effects on the 6th day. It indicated that GABA sprayed on the leaves of leek could be absorbed, which caused the decrease in nitrate content of leek by inducing the key enzyme activity of NR, NiR and other in inorganic nitrogen metabolism, and finally improved the yield and quality of leek.

Key words: GABA, leek, growth, nitrate