bZIP转录因子在植物激素介导的抗病抗逆途径中的作用

doi:10.3969/j.issn.1004-1524.2017.01.23

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 168-175.DOI: 10.3969/j.issn.1004-1524.2017.01.23

• 综述 • 上一篇

bZIP转录因子在植物激素介导的抗病抗逆途径中的作用

李冬月^{1, 2}, 原文霞^{1, 2}, 郑超^{1, 2}, 王栩鸣², 周洁², 严成其^{1, 2, *}, 陈剑平^{1, 2, *}

1.浙江师范大学化学与生命科学学院,浙江金华321004;
2.浙江省农业科学院病毒学与生物技术研究所,浙江省有害生物防控国家重点实验室培育基地,农业部植保生物技术重点实验室,浙江省植物病毒重点实验室,浙江杭州310021

收稿日期:2016-04-22 出版日期:2017-01-15 发布日期:2017-02-23
通讯作者: 严成其,E-mail:yanchengqi@163.com;陈剑平,E-mail:jpchen2001@126.com
作者简介:李冬月(1990—),女,河南南阳人,硕士研究生,主要从事植物病理学研究。E-mail:dongyueli2015@163.com
基金资助:
国家科技部973前期(2014CB160309); 浙江省自然科学基金(2014C140001,2013C11009)

Roles of bZIP transcription factors in phytohormone-mediated disease resistance and stress tolerance

LI Dongyue^{1, 2}, YUAN Wenxia^{1, 2}, ZHENG Chao^{1, 2}, WANG Xuming², ZHOU Jie², YAN Chengqi^{1, 2, *}, CHEN Jianping^{1, 2, *}

1. College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,MOA Key Laboratory for Plant Protection and Biotechnology,Zhejiang Provincial Key Laboratory of Virology,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China

Received:2016-04-22 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 植物体内有一套复杂精细且交叉调控的激素信号网络,在植物应对不良环境胁迫的过程中起着至关重要的作用。bZIP转录因子作为基因表达的关键调控因子,在植物抗病抗逆相关基因的表达中行使着重要的功能。文章首先介绍了bZIP转录因子的结构和功能,阐述了其在几种主要的激素抗性调控途径中的作用机理,之后对各种激素信号通路之间的交叉调控途径及其关键调控节点蛋白进行了概括,以期阐明植物激素介导的抗性调控网络,并为新的抗性相关基因的挖掘和利用提供理论依据。

关键词: bZIP转录因子, 植物激素, 抗病, 抗逆, 信号调控

Abstract: The elaborate phytohormone regulating networks in plants and their crosstalk pathways play critical roles in adapting to various environmental stresses of plants. As the key regulators in gene expression, bZIP transcription factors control important processes in relation to plant biotic and abiotic stress responses. In this review, we firstly introduced the structure and functions of the bZIP transcription factors, expounded their regulation mechanism in several major hormone-mediated signaling pathways. Subsequently, we discussed the crosstalk pathways between different hormones and made a summary of the key crosstalk integrators in defense processes. The aim of this review was to illuminate plant hormone-mediated signal interactions under stress and provide theoretical bases for exploring and utilizing new resistance genes.

Key words: bZIP transcription factors, plant hormones, disease resistance, stress tolerance, signal regulation

中图分类号:

S184
Q945.8

李冬月, 原文霞, 郑超, 王栩鸣, 周洁, 严成其, 陈剑平. bZIP转录因子在植物激素介导的抗病抗逆途径中的作用[J]. 浙江农业学报, 2017, 29(1): 168-175.

LI Dongyue, YUAN Wenxia, ZHENG Chao, WANG Xuming, ZHOU Jie, YAN Chengqi, CHEN Jianping. Roles of bZIP transcription factors in phytohormone-mediated disease resistance and stress tolerance[J]. , 2017, 29(1): 168-175.

参考文献

[1] PACIFICI E, POLVERARI L, SABATINI S. Plant hormone cross-talk: the pivot of root growth[J]. Journal of Experimental Botany, 2015, 66(4):1113-1121.
[2] 成晓越, 王栩鸣, 杨勇, 等. 一氧化氮参与疣粒野生稻对水稻白叶枯病的抗性[J]. 浙江农业学报, 2014, 26(1): 1-6.
CHENG X Y, WANG X M, YANG Y, et al. Ntiric oxide involved in defense responses of Oryza meyeriana to rice bacterial blight[J]. Acta Agriculturae Zhejiangensis, 2014, 26(1): 1-6. (in Chinese with English abstract)
[3] LUTOVA L A, DODUEVA I E, LEBEDEVA M A, et al. Transcription factors in developmental genetics and the evolution of higher plants[J]. Russian Journal of Genetics, 2015, 51(5): 449-466.
[4] YANG S D, SEO P J, YOON H K, et al. The Arabidopsis NAC transcription factor VNI2 integrates abscisic acid signals into leaf senescence via the COR/RD genes[J]. Plant Cell, 2011, 23(6): 2155-2168.
[5] WANG Z, TANG J, HU R, et al. Genome-wide analysis of the R2R3-MYB transcription factor genes in Chinese cabbage (Brassica rapa ssp. pekinensis) reveals their stress and hormone responsive patterns[J]. BMC Genomics, 2015, 16(1): 1-21.
[6] 刘忠丽, 丛悦玺, 苟维超, 等. MYB类转录因子在植物细胞生长发育中的作用及其应用[J]. 浙江农业学报, 2012, 24(1):174-179.
LIU Z L, CONG Y X, GOU W C, et al. The role and application of MYB-type transcription factors in the plant growth and development[J]. Acta Agriculturae Zhejiangensis, 2012, 24(1):174-179. (in Chinese with English abstract)
[7] MASAKI S, HIRON0RI K, AKAGI, et al. Rice WRKY45 plays important roles in fungal and bacterial disease resistance[J]. Molecular Plant Pathology, 2012, 13(1): 83-94.
[8] FUJISAW, BABA T, NAGAMURA Y, et al. The map-based sequence of the rice genome[J]. Nature, 2005, 436(7052): 793-800.
[9] INITIATIVE A G. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana[J]. Nature, 2000, 408(6814): 796-815.
[10] VELASCO R, ZHARKIKH A, AFFOURTIT J, et al. The genome of the domesticated apple (Malus × domestica Borkh)[J]. Nature Genetics, 2010, 42(10): 833-839.
[11] LEE S C, CHOI H W, HWANG I S, et al. Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses[J]. Planta, 2006, 224(5): 1209-1225.
[12] SCHUMACHER M A, GOODMAN R H, BRENNAN R G. The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding[J]. Journal of Biological Chemistry, 2000, 275(45): 35242-35247.
[13] LIU L S, MICHAEL J, WHITE T, et al. Transcription factors and their genes in higher plants[J]. European Journal of Biochemistry, 1999, 262(2): 247-257.
[14] CHARLES D, CATHERINE C, AMANDA R, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1[J]. Plant Cell, 2003, 15(9): 2181-2191.
[15] NIJHAWAN A, JAIN M, TYAGI A K, et al. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice[J]. Plant Physiology, 2008, 146(2): 333-350.
[16] MARC J, BERND W, WOLFGANG D, et al. bZIP transcription factors in Arabidopsis[J]. Trends in Plant Science, 2002, 7(3): 106-111.
[17] CHOI H, HONG J, HA J, et al. ABFs, a family of ABA-responsive element binding factors[J]. Journal of Biological Chemistry, 2000, 275(3): 1723-1730.
[18] KESARWANI M, YOO J X. Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis[J]. Plant Physiology, 2007, 144(1): 336-346.
[19] RUGNER A, FROHNMEYER H, NAKE C, et al. Isolation and characterization of four novel parsley proteins that interact with the transcriptional regulators CPRF1 and CPRF2[J]. Molecular Genetics & Genomics, 2001, 265(6): 964-976.
[20] ANJA S, PETRA Z, ULRIKE Z. G-box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis[J]. Plant Physiology, 2010, 153(3): 1321-1331.
[21] BAENA-GONZÁLEZ E, ROLLAND F, THEVELEIN J M, et al. A central integrator of transcription networks in plant stress and energy signalling[J]. Nature, 2007, 448(7156): 938-942.
[22] MURILO S, ALVES S, AMANDA B, et al. Plant bZIP transcription factors responsive to pathogens: A review[J]. International Journal of Molecular Sciences, 2013, 14(4): 7815-7828.
[23] YASUNARI F, MIKI F, RIE S, et al. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis[J]. Plant Cell, 2005, 17(12): 3470-3488.
[24] ZOU M, GUAN Y, REN H, et al. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance[J]. Plant Molecular Biology, 2008, 66(6): 675-683.
[25] LIU C, MAO B, OU S, et al. OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice[J]. Plant Molecular Biology, 2014, 84(1-2): 19-36.
[26] SHAH J. The salicylic acid loop in plant defense[J]. Current Opinion in Plant Biology, 2003, 6(4): 365-371.
[27] STICHER L, MAUCH-MANI B, MÉTRAUX J P. Systemic acquired resistance[J]. Annual Review of Phytopathology,1997, 35: 235-270.
[28] FU Z Q, DONG X. Systemic acquired resistance: turning local infection into global defense[J]. Annual Review of Plant Biology, 2013, 64(4): 839-863.
[29] MOU Z L, FAN W H, DONG X N. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell, 2003, 113(7): 935-944.
[30] YASUOMI T S, STVVEN H, KAROLINA P M, et al. Plant immunity requires conformational changes of NPR1 via S-nitrosylation and thioredoxins[J]. Science, 2008, 321(5891): 952-956.
[31] ZHANG Y L, TESSARO M M, LI X. KnoCTKout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance[J]. Plant Cell, 2003, 15(11): 2647-2653.
[32] FITZGERALD H A, CANLAS P E, CHERN M S, et al. Alteration of TGA factor activity in rice results in enhanced tolerance to Xanthomonas oryzae pv. oryzae[J]. Plant Journal, 2005, 43(3): 335-347.
[33] ZHAO J, GUO R R, GUO C L, et al. Evolutionary and expression analyses of the apple basic leucine zipper transcription factor family[J]. Frontiers in Plant Science, 2016,7:376.
[34] ZHANG Y P, ZHOU J H, WANG L, et al. Mini review roles of the bZIP gene family in rice[J]. Genetics & Molecular Research, 2014, 13(2): 3025-3036.
[35] CREELMAN R A, MULLET J E. Biosynthesis and Action of Jasmonates in Plants[J]. Annual Review of Plant Biology, 1997, 48: 355-381.
[36] GYU I, LEE H, GREGG A. The tomato mutant spr1 is defective in systemin perception and the production of a systemic wound signal for defense gene expression[J]. Plant Journal for Cell & Molecular Biology, 2003, 33(3): 567-576.
[37] HOWE G A. Jasmonates as signals in the wound response[J]. Journal of Plant Growth Regulation, 2004, 23(3): 223-237.
[38] MARK Z, SYLVAIN L C, OLIVIER L, et al. Arabidopsis thaliana class-II TGA transcription factors are essential activators of jasmonic acid ethylene-induced defense responses[J]. The Plant Journal, 2010, 2010(61): 200-210.
[39] IRIS A, PENNINCTKX A, BART P, et al. Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis[J]. Plant Cell, 1998, 10(12): 2103-2113.
[40] 沈爱华, 罗红兵, 邓志平, 等. 油菜素内酯信号传递在水稻中的研究进展[J]. 浙江农业学报, 2014, 26(5): 1399-1404.
SHEN A H, LUO H B, DENG Z P, et al. Recent advances in brassinosteroid signaling in rice[J]. Acta Agriculturae Zhejiangensis, 2014, 26(5): 1399-1404. (in Chinese with English abstract)
[41] KIM B, FUJIOKA S, KWON M, et al. Arabidopsis brassinosteroid-overproducing gulliver3-D/dwarf4-D mutants exhibit altered responses to jasmonic acid and pathogen[J]. Plant Cell Reports, 2013, 32(7): 1139-1149.
[42] CHUNG Y, KWON S I, CHOE S. Antagonistic regulation of Arabidopsis growth by brassinosteroids and abiotic stresses[J]. Molecules & Cells, 2014, 37(8): 1165-1169.
[43] ZANDER M, THUROW C, GATZ C. TGA Transcription factors activate the salicylic acid-suppressible branch of the ethylene-induced defense program by regulating ORA59 expression[J]. Plant Physiology, 2014, 165(4): 1671-1683.
[44] TANG N, ZHANG H, LI X, et al. Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice[J]. Plant Physiology, 2012, 158(4): 1755-1768.
[45] AMIR H M, LEE Y, CHO J I, et al. The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice[J]. Plant Molecular Biology, 2010, 72(4-5): 557-566.
[46] MUKHERJEE K, CHOUDHURY A R, GUPTA B, et al. An ABRE-binding factor, OSBZ8, is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice[J]. BMC Plant Biology, 2006, 104(25):1466-1470.
[47] ZOU M, GUAN Y, REN H, et al. Characterization of alternative splicing products of bZIP transcription factors OsABI5[J]. Biochemical & Biophysical Research Communications, 2007, 360(2): 307-313.
[48] ZOU M, GUAN Y, REN H, et al. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance[J]. Plant Molecular Biology, 2008, 66(6): 675-683.
[49] JI Y H, JU C M, IN S L, et al. Phosphorylation-mediated regulation of a rice ABA responsive element binding factor[J]. Phytochemistry, 2011, 72(1): 27-36.
[50] LIU C, WU Y, WANG X. bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice[J]. Planta, 2011, 235(6): 1157-1169.
[51] LU G, GAO C, ZHENG X, et al. Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice[J]. Planta, 2009, 229(3): 605-615.
[52] KESHISHIAN E A, RASHOTTE A M. Plant cytokinin signalling[J]. Essays in Biochemistry, 2015, 58: 13-27.
[53] GUPTA S, RASHOTTE A M. Down-stream components of cytokinin signaling and the role of cytokinin throughout the plant[J]. Plant Cell Reports, 2012, 31(5): 801-812.
[54] TOMÁS W, ERIKA N, IREEN K L, et al. Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco[J]. Plant Cell, 2010, 22(12): 3905-3920.
[55] ARGYROS R D, MAEHEWS D E, Chiang Y H, et al. Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development[J]. Plant Cell, 2008, 20(8): 2102-2118.
[56] HWANG I, SHEEN J, MÜLLER B. Cytokinin signaling networks[J]. Annual Review of Plant Biology, 2012, 63: 353-380.
[57] NGUYEN K H, HA C V, NISHIYAMA R, et al. Arabidopsis type B cytokinin response regulators ARR1, ARR10, and ARR12 negatively regulate plant responses to drought[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016,113(11):3090-3095.
[58] PELEG Z, BLUMWALD E. Hormone balance and abiotic stress tolerance in crop plants[J]. Current Opinion in Plant Biology, 2011, 14(3): 290-295.
[59] ZWACTK J P, RASHOTTE M A. Interactions between cytokinin signalling and abiotic stress responses[J]. Journal of Experimental Botany, 2015, 66(16): 4863-4871.
[60] CHOI J, HUH S U, KOJIMA M, et al. The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis[J]. Developmental Cell, 2010, 19(2): 284-295.
[61] MUHAMMAD N, THOMAS D. The role of auxin-cytokinin antagonism in plant pathogen interactions[J]. Plos Pathogens, 2012, 8(11): 1352-1362.
[62] O'BRIEN J A, BENKOVA E. Cytokinin cross-talking during biotic and abiotic stress responses[J]. Frontiers in Plant Science, 2013, 4: 451.
[63] DORON S I, DUDY B Z. ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation by reducing polar auxin transport in Arabidopsis[J]. Plant Cell, 2010, 22(11): 3560-3573.
[64] WIND J J, ALESSIA P, BEREND S, et al. ABI4: versatile activator and repressor[J]. Trends in Plant Science, 2013, 18(3): 125-132.
[65] HWANG I, SHEEN J. Two-component circuitry in Arabidopsis cytokinin signal transduction[J]. Nature, 2001, 413(6854): 383-389.
[66] LI W, DEPING H, JUNNA H, et al. Auxin response factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis[J]. Plos Genetics, 2011, 7(7): e1002172.
[67] YITING S, SHOUWEI T, LINGYAN H, et al. Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis[J]. Plant Cell, 2012, 24(6): 2578-2595.
[68] RUZICTKA K, LJUNG K, VANNESTE S, et al. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution[J]. Plant Cell, 2007, 19(7): 2197-2212.
[69] ZHU Z, AN F, FENG Y, et al. Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis[J]. Proceeding of National Academy of Sciences of the United States of America, 2011, 108(30): 12539-12544.
[70] ZAREI A, KORBES A P, YOUNESSI P, et al. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis[J]. Plant Molecular Biology, 2011, 75(4-5): 321-331.
[71] HYUN S C, FRANCOIS F, KIEBER J J. The eto1, eto2, and eto3 mutations and cytokinin treatment increase ethylene biosynthesis in Arabidopsis by increasing the stability of ACS protein[J]. Plant Cell, 2003, 15(2): 545-559.
[72] MOHR P G, CAHILL D M. Suppression by ABA of salicylic acid and lignin accumulation and the expression of multiple genes, in Arabidopsis infected with Pseudomonas syringae pv. tomato[J]. Functional & Integrative Genomics, 2007, 7(3): 181-191.
[73] MICHIKO Y, ATSUSHI I, YUSUKE J, et al. Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis[J]. Plant Cell, 2008, 20(6): 1678-1692.
[74] MOSHER S, MOEDER M, NISHIMURA N, et al. The lesion-mimic mutant cpr22 shows alterations in abscisic acid signaling and abscisic acid insensitivity in a salicylic acid-dependent manner[J]. Plant Physiology, 2010, 152(4): 1901-1913.

bZIP转录因子在植物激素介导的抗病抗逆途径中的作用

Roles of bZIP transcription factors in phytohormone-mediated disease resistance and stress tolerance

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	岳建华, 董艳, 李文杨, 李蒙, 张琰. pH对百子莲体胚诱导期生理特性的影响[J]. 浙江农业学报, 2020, 32(8): 1405-1414.
[2]	邱文怡, 王诗雨, 李晓芳, 徐恒, 张华, 朱英, 王良超. MYB转录因子参与植物非生物胁迫响应与植物激素应答的研究进展[J]. 浙江农业学报, 2020, 32(7): 1317-1328.
[3]	王峰, 叶静, 高敬文, 王强, 俞巧钢, 何新华, 马军伟. 外源增钾缓解铵胁迫下小麦根系受抑[J]. 浙江农业学报, 2020, 32(11): 1923-1933.
[4]	徐莉, 陈小洁, 曹静婷, 刘楚楚, 丁婷, 江腾. 小麦赤霉病生防菌DZSG23的抗病机制[J]. 浙江农业学报, 2020, 32(11): 2001-2008.
[5]	刘慧洁, 徐恒, 邱文怡, 李晓芳, 张华, 朱英, 李春寿, 王良超. 转录因子在植物生长发育及非生物逆境响应的作用[J]. 浙江农业学报, 2019, 31(7): 1205-1214.
[6]	时羽杰, 李兴龙, 唐媛, 余海萍, 邬晓勇. 基于GC-MS分析两地白色藜麦种子的代谢差异[J]. 浙江农业学报, 2019, 31(6): 869-877.
[7]	何海燕, 柴荣耀, 邱海萍, 毛雪琴, 王艳丽, 孙国仓. 五个抗稻瘟病基因在浙江省水稻品种中的分布和抗性评价[J]. 浙江农业学报, 2019, 31(6): 922-929.
[8]	王掌军, 刘妍, 张双喜, 刘凤楼, 李清峰, 张晓岗, 刘生祥, 贾彪. 宁春4号与河东乌麦杂交F₂代抗病性及分子标记鉴定[J]. 浙江农业学报, 2019, 31(5): 677-687.
[9]	王其, 陈小洁, 顾双月, 张欣悦, 杭天露, 丁婷. 杜仲内生拮抗细菌DZSY21诱导玉米抗病基因表达变化的转录组学研究[J]. 浙江农业学报, 2019, 31(3): 345-354.
[10]	康海婷, 凌丹燕, 吴志娟, 路梅, 宗宇, 郭卫东. 蓝莓枝干溃疡病病原鉴定及品种抗病性研究[J]. 浙江农业学报, 2019, 31(3): 436-443.
[11]	戴超辉, 冯海悦, 吴圣龙, 包文斌. 猪源Toll样受体家族(TLRs)及其在抗病育种中的应用[J]. 浙江农业学报, 2018, 30(3): 507-520.
[12]	陈远松, 朱晓伟, 龚翔宇, 梅至诚, 祝彪. 分子标记在我国番茄抗病育种的应用研究进展[J]. 浙江农业学报, 2017, 29(8): 1415-1420.
[13]	徐爽, 吴曼莉, 柯智健, 柳志强, 李晓宇. 胶孢炭疽菌CgRGS4调控营养生长、渗透压响应、氧化应激反应和致病性[J]. 浙江农业学报, 2017, 29(2): 277-285.
[14]	陈颖, 郑圣晗, 孔令琳, 朱鹏飞, 吴允, 郭其新, 陈国宏, 常国斌. 不同鸡品种BNK基因序列比较分析[J]. 浙江农业学报, 2017, 29(10): 1648-1653.
[15]	王丽丽，葛金涛，刘兴满，赵统利*. 葡萄miR164家族生物信息学分析及靶基因预测[J]. 浙江农业学报, 2016, 28(3): 441-.