苹果YABBY基因家族的鉴定、进化及表达分析

doi:10.3969/j.issn.1004-1524.2017.07.10

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1129-1138.DOI: 10.3969/j.issn.1004-1524.2017.07.10

苹果YABBY基因家族的鉴定、进化及表达分析

邵红霞, 陈鸿飞, 张东, 吴海芹, 赵彩平, 韩明玉^*

西北农林科技大学园艺学院,陕西杨凌 712100

收稿日期:2017-02-27 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 韩明玉,E-mail:hanmy@nwsuaf.edu.cn
作者简介:邵红霞(1991—),女,陕西定边人,硕士研究生,研究方向为苹果花芽分化机制。E-mail:xnyyshx@163.com
基金资助:
国家科技支撑计划项目(2013BAD20B03); 国家星火计划项目(2014GA850002); 国家现代农业产业技术体系项目(CARS-28),国家苹果改良中心杨凌分中心,陕西省农业科技创新与攻关项目(2015NY114); 陕西省果业发展协同中心和陕西果业发展项目

Identification, evolution and expression analysis of the YABBY gene family in apple (Malus×domestica Borkh.)

SHAO Hongxia, CHEN Hongfei, ZHANG Dong, WU Haiqin, ZHAO Caiping, HAN Mingyu^*

College of Horticulture, Northwest A &F University, Yangling 712100, China

Received:2017-02-27 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： 从苹果全基因组范围内共鉴定出13个YABBY基因,通过聚类分析将其分为YAB1/YAB3、YAB2、INO、YAB5和CRC 5个亚家族。MdYABBY基因分布在苹果的9条染色体上,在第16条染色体上分布最多。MdYABBY蛋白长度介于60~538个氨基酸,等电点为4.84~9.81,所有MdYABBY蛋白都包含有锌指结构和YABBY 两个保守结构域,同一个亚家族内的成员表现出相似的基因结构和蛋白保守基序分布;基因顺式作用元件分析发现,MdYABBY基因包含许多与抗逆和激素响应有关的作用元件。基因表达分析发现,MdYABBY基因在花、果和叶中的表达量较高,5个MdYABBY基因在果实发育的3个时期均明显地上调表达。

关键词: 苹果, YABBY基因, 聚类分析, 进化, 基因表达

Abstract: 13 Malus×domestica YABBY genes were characterized and divided into five groups (YAB1/YAB3, YAB2, INO, YAB5 and CRC). All the M. domestica YABBY genes distributed on nine chromosomes in which the sixteenth contained the maximum amount of members. The length and isoelectric points of M. domestica YABBY proteins were from 60 to 538 amino acids and 4.84 to 9.81, respectively. All the 13 M. domestica YABBY proteins had a zinc finger-like domain in the N terminus and a YABBY domain in the C terminus, and the members of one subgroup present similar gene structure and conserved protein motifs, then the cis-elements analysis found that there were many cis-elements related to stress and hormone response. Gene expression analysis indicated that most of the M. domestica YABBY genes were highly expressed in flower, fruits and leaves. Five MdYABBY genes were all greatly up-regulated during three fruit development.

Key words: apple, YABBY gene, cluster analysis, evolution, gene expression

中图分类号:

S661.1

邵红霞, 陈鸿飞, 张东, 吴海芹, 赵彩平, 韩明玉. 苹果YABBY基因家族的鉴定、进化及表达分析[J]. 浙江农业学报, 2017, 29(7): 1129-1138.

SHAO Hongxia, CHEN Hongfei, ZHANG Dong, WU Haiqin, ZHAO Caiping, HAN Mingyu. Identification, evolution and expression analysis of the YABBY gene family in apple (Malus×domestica Borkh.)[J]. , 2017, 29(7): 1129-1138.

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苹果YABBY基因家族的鉴定、进化及表达分析

Identification, evolution and expression analysis of the YABBY gene family in apple (Malus×domestica Borkh.)

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