红颜草莓脱毒优化及病毒检测的研究

doi:10.3969/j.issn.1004-1524.2017.06.15

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 966-970.DOI: 10.3969/j.issn.1004-1524.2017.06.15

红颜草莓脱毒优化及病毒检测的研究

陈英, 肖春林, 罗燕娜, 张西英, 刘江娜

新疆生产建设兵团第六师农业科学研究所,新疆五家渠 831300

收稿日期:2017-02-09 出版日期:2017-06-20 发布日期:2017-09-07
作者简介:陈英(1967—),女,湖北黄陂人,高级农艺师,主要从事特色作物脱毒快繁、育苗栽培技术的研究与应用。E-mail: 1131382130@qq.com
基金资助:
国家十二五科技支撑计划项目(2012BAD41B00)

Detoxification protocal optimization and virus detection in strawberry variety Hongyan

CHEN Ying, XIAO Chunlin, LUO Yanna, ZHANG Xiying, LIU Jiangna

Research Institute of Agricultural Sciences, the 6th Agricultural Production Division of Xinjiang Production and Construction Corps, Wujiaqu 831300, China

Received:2017-02-09 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 以红颜草莓品种为材料,采用无菌瓶苗,经高温处理、冰点低温处理结合茎尖剥离脱毒,并用血清学中的双抗体一步夹心酶联免疫法检测4种病毒的方法,对细胞分生组织诱导分化成苗的成活率高低和脱毒效果进行比较。结果表明,采用-1 ℃低温处理结合茎尖剥离长度由0.3 mm增加到0.5 mm,诱导分化苗的成活率由23.0%大幅度提高到39.6%~60.0%,再通过双抗体夹心酶联免疫法检测病毒,4种病毒全部脱除。

关键词: 草莓, 温度, 茎尖脱毒, 病毒检测, 优化

Abstract: With strawberry variety Hongyan as the material, the sterile container seedlings were treated with high temperature treatment, low temperature freezing treatment combined with stem tip off detoxification. The double antibody step of serology and the clip art enzyme-linked immunoassay detection method were carried out to detect four kinds of virus. The survival rate and the detoxification efficiency of seedlings induced by meristem cells were compared. The results showed that the survival rate increased from 23.0% to 39.6%-60.0% under-1 ℃ cryogenic treatment with the stem tip stripping length increased from 0.3 mm to 0.5 mm, and all four virus were removed by the double antibody sandwich enzyme-linked immunoassay detection.

Key words: strawberry, temperature, virus-free meristem, virus detection, optimization

中图分类号:

S668.4

陈英, 肖春林, 罗燕娜, 张西英, 刘江娜. 红颜草莓脱毒优化及病毒检测的研究[J]. 浙江农业学报, 2017, 29(6): 966-970.

CHEN Ying, XIAO Chunlin, LUO Yanna, ZHANG Xiying, LIU Jiangna. Detoxification protocal optimization and virus detection in strawberry variety Hongyan[J]. , 2017, 29(6): 966-970.

参考文献

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红颜草莓脱毒优化及病毒检测的研究

Detoxification protocal optimization and virus detection in strawberry variety Hongyan

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