镉胁迫对铁皮石斛叶片抗氧化酶活性的影响及动力学分析

doi:10.3969/j.issn.1004-1524.2017.09.01

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1421-1429.DOI: 10.3969/j.issn.1004-1524.2017.09.01

镉胁迫对铁皮石斛叶片抗氧化酶活性的影响及动力学分析

姜武, 吴志刚, 陈松林, 陶正明^*

浙江省农业科学院浙江省亚热带作物研究所,浙江温州 325005

收稿日期:2017-03-17 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 陶正明,E-mail:zmtao2002@aliyun.com.cn
作者简介:姜武(1987—),男,浙江温州人,硕士,助理研究员,研究方向为中药栽培技术与药材质量研究。E-mail:jiangwu8888@163.com
基金资助:
温州市公益性农业科技项目(N20150017); 浙江省农业科学院地方科技合作项目(WZ20130006,CA20150005); 浙江省农业技术推广中心中药材产业技术团队项目(浙农技[2016]54号)

Kinetic analysis and antioxidant activity of Dendrobium officinale leaves under cadmium stress

JIANG Wu, WU Zhigang, CHEN Songlin, TAO Zhengming^*

Zhejiang Institute of the Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China

Received:2017-03-17 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 为探究长期镉胁迫对铁皮石斛抗氧化能力的影响,对铁皮石斛叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性在不同浓度梯度镉胁迫中的响应关系进行研究,并建立基于logistic函数的镉胁迫SOD、CAT、POD活性变化动力学方程。结果表明:SOD、CAT、POD活性与镉浓度呈正相关,一定浓度的镉胁迫有利于提高铁皮石斛抗氧化酶活性。结合试验结果得到的模型参数表明,各胁迫水平下建立的12个模型拟合度好(R²>0.9,P<0.05);镉胁迫浓度与铁皮石斛叶片抗氧化酶活性内禀增长率呈正相关,与环境容纳极限值和拐点时刻呈负相关;综合各模型拐点时刻及铁皮石斛生长周期,镉处理促进铁皮石斛抗氧化酶的极限浓度为2 mg·L^-1。因此,低浓度镉胁迫对铁皮石斛毒害较低,同时能一定程度上增加抗氧化酶的积累;logistic动力学模型可较好地反映镉胁迫下铁皮石斛叶片的动态生长过程。

关键词: 铁皮石斛, 镉胁迫, 抗氧化酶, 动力学模型

Abstract: In order to study the effects of cadmium (Cd) stress on antioxidant capacity of Dendrobium officinale, experiments of response relationship between Cd stress and superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in D. officinale leaves were done, and dynamics equation of SOD, CAT, POD activities based on logistic function were established. The results showed that there was a positive correlation between the SOD, CAT, POD activities and Cd concentration, and the antioxidant capacity was increased when treated by certain Cd concentration. The model parameter calculation displayed that the fitting degree of 12 model under different stress level were fine (R²>0.9, P<0.05). The model parameter calculation indicated that Cd concentration was positively correlation with the intrinsic rates, while negatively correlated with the environmental capacity and inflection points. Comprehensive D. officinale growth period and all inflection points showed that the limit Cd concentration was 2 mg·L^-1 which could improve antioxidant accumulation. Thus, low concentration of Cd stress not only had low toxicity to D. officinale, but also could increase the antioxidant accumulation to a certain extent. Dynamic model based on logistic function with Cd concentration could be a potential tool for predicting the dynamic process of D. officinale growth under Cd stress.

Key words: Dendrobium officinale, cadmium stress, antioxidant enzyme, dynamics model

中图分类号:

S567.23⁺9

姜武, 吴志刚, 陈松林, 陶正明. 镉胁迫对铁皮石斛叶片抗氧化酶活性的影响及动力学分析[J]. 浙江农业学报, 2017, 29(9): 1421-1429.

JIANG Wu, WU Zhigang, CHEN Songlin, TAO Zhengming. Kinetic analysis and antioxidant activity of Dendrobium officinale leaves under cadmium stress[J]. , 2017, 29(9): 1421-1429.

参考文献

[1] 国家药典委员会. 中国药典, 一部[M]. 北京: 中国医药科技出版社, 2015: 282-283.
[2] 吕圭源, 颜美秋, 陈素红. 铁皮石斛功效相关药理作用研究进展[J]. 中国中药杂志, 2014, 38(4): 489-493.
LYU G Y, YAN M Q, CHEN S H. Review of pharmacological activities of Dendrobium officinale based on traditional functions[J]. China Journal of Chinese Materia Media , 2014, 38(4): 489-493. (in Chinese with English abstract)
[3] 雷珊珊, 吕圭源, 金泽武, 等. 铁皮石斛花提取物对甲亢型阴虚小鼠的影响[J]. 中国中药杂志, 2015, 40(9): 1793-1797.
LEI S S, LYU G Y, JIN Z W, et al. Effect of extracts from Dendrobium officinale flos on hyperthyroidism Yin deficiency mice [J]. China Journal of Chinese Materia Media , 2015, 40(9): 1793-1797. (in Chinese with English abstract)
[4] 斯金平, 何伯伟, 俞巧仙. 铁皮石斛良种选育进展与对策[J]. 中国中药杂志, 2013, 38(4): 475-480.
SI J P, HE B W, YU Q X. Progress and countermeasures of Dendrobium officinale breeding [J]. China Journal of Chinese Materia Media , 2013, 38(4): 475-480. (in Chinese with English abstract)
[5] 姜武, 陈松林, 吴志刚, 等. 人工栽培铁皮石斛品质评价[J]. 浙江农业科学, 2016, 57(6): 836-837.
JIANG W, CHEN S L, WU Z G, et al. The quality evaluation of cultivation Dendrobium officinale [J]. Journal of Zhejiang Agricultural Sciences , 2016, 57(6): 836-837. (in Chinese)
[6] 何伯伟, 潘慧锋. 浙江铁皮石斛产业提升发展的实施措施与建议[J]. 浙江农业科学, 2014 (2): 152-155.
HE B W, PAN H F. The promote development of the implementation of the measures and suggestions in Zhejiang Dendrobium officinale industry [J]. Journal of Zhejiang Agricultural Sciences , 2014 (2): 152-155. (in Chinese)
[7] 斯金平, 俞巧仙, 宋仙水, 等. 铁皮石斛人工栽培模式[J]. 中国中药杂志, 2013, 38(4): 481-484.
SI J P, YU Q X, SONG M Q, et al. Artificial cultivation modes for Dendrobium officinale [J]. China Journal of Chinese Materia Media , 2013, 38(4): 481-484. (in Chinese with English abstract)
[8] 徐丽红, 郑蔚然, 王小骊, 等. 铁皮石斛产地环境及产品中重金属的监测与污染评价[J]. 浙江农业学报, 2015, 27(3): 429-433.
XU L H, ZHENG W R, WANG X L, et al. Monitoring and evaluation for heavy metals for Dendrobium officinale Kimura et Migo and its origin environment[J]. Acta Agriculturae Zhejiangenis , 2015, 27(3): 429-433. (in Chinese with English abstract)
[9] 蒋汉明, 李书启, 韩希凤, 等. 镉对植物生长的影响及植物耐镉机理研究进展[J]. 广东微量元素科学, 2012, 19(5): 1-6.
JIANG H M, LI S Q, HAN X F, et al. Progresses on the effect of cadmium for plant and their tolerant mechanism[J]. Guangdong Trace elements Science , 2012, 19(5): 1-6. (in Chinese with English abstract)
[10] 逄洪波, 谷思雨, 李玥莹, 等. 镉超富集植物耐镉性的分子机制研究进展[J]. 北方园艺, 2015 (19): 170-174.
PANG H B, GU S Y, LI Y Y, et al. Research progress on the molecular mechanism underlying cadmium hyperaccumulation tolerance[J]. Northern Horticulture , 2015 (19): 170-174. (in Chinese with English abstract)
[11] 刘明浩, 陈光辉, 王悦. 植物耐镉机制研究进展[J]. 作物研究, 2015, 29(1): 101-105.
LIU M H, CHEN G H, WANG Y. Research progress on mechanisms of plant resistance to cadmium[J]. Crop Research , 2015, 29(1): 101-105. (in Chinese with English abstract)
[12] 王连臻. 镉对小麦苗期生长及生理指标的影响[J]. 安徽农业科学, 2008, 36(9): 3529-3530.
WANG L Z. Study on the effects of cadmium on the seedling growth and the physiological indexes of wheat in seedling stage[J]. Journal of Anhui Agricultural Sciences , 2008, 36(9): 3529-3530. (in Chinese with English abstract)
[13] 姜武, 吴志刚, 陶正明. 铁皮石斛的本草考证[J]. 中药材. 2014, 37(4): 697-699.
JIANG W, WU Z G, TAO Z M. Textual identification of Dendrobium officinale [J]. Journal of Chinese Medicinal Materials , 2014, 37(4): 697-699. (in Chinese)
[14] 白音, 包英华, 王文全, 等. 不同居群美花石斛种质资源的RAPD分析[J]. 中草药, 2007, 38(5): 748-751.
BAI Y, BAO Y H, WANG W Q, et al. RAPD analysis on germplasm resources in different population of Dendrobium loddigesii [J]. Chinese Traditional and Herbal Drugs , 2007, 38(5): 748-751. (in Chinese with English abstract)
[15] 丁鸽, 丁小余, 沈洁, 等. 铁皮石斛野生居群遗传多样性的RAPD分析与鉴别[J]. 药学学报, 2005, 40(11): 1028-1032.
DING G, DING X Y, SHEN J, et al. Genetic diversity and molecular authentication of wild populations of Dendrobium officinale by RAPD[J]. Acta Pharmaceutica Sinica , 2005, 40(11): 1028-1032. (in Chinese with English abstract)
[16] 王慧中, 卢江杰, 施农农, 等. 13种石斛属植物遗传多样性的AFLP分析[J]. 分子细胞生物学报, 2007, 40(3): 205-209.
WANG H Z, LU J J, SHI N N, et al. Analysis of genetic diversity among 13 Chinese species of Dendrobium based on AFLP[J]. Journal of Molecular Cell Biology , 2007, 40(3): 205-209. (in Chinese with English abstract)
[17] LI X, DING X, CHU B, et al. Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP[J]. Genetica , 2008, 133(2): 159-166.
[18] DING G, XU G, ZHANG W, et al. Preliminary geoherbalism study of Dendrobium officinale food by DNA molecular markers[J]. European Food Research and Technology , 2008, 227(4): 1283-1286.
[19] DING G, ZHANG D, DING X, et al. Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis[J]. Plant Systematics and Evolution , 2008, 276(3/4): 149-156.
[20] 周桂芬,吕圭源. 铁皮石斛不同部位黄酮碳苷类成分及清除DPPH自由基能力比较研究[J]. 中国中药杂志, 2012, 37(11): 1536-1540.
ZHOU G F, LYU G Y. Comparative studies on scavenging DPPH free redicals activity of flavone C-glycosides from different parts of Dendrobium officinale [J]. China Journal of Chinese Materia Media , 2012, 37(11): 1536-1540. (in Chinese with English abstract)
[21] 李巧自, 高素萍, 张科燕, 等. 外源一氧化氮对铁皮石斛原球茎生长和多糖积累的影响[J]. 浙江农业学报, 2016, 28(4): 595-600.
LI Q Z, GAO S P, ZHANG K Y, et al. Effects of exogenous nitric oxide on growth and polysaccharide accumulation of protocorm-like bodies from Dendrobium officinale [J]. Acta Agriculturae Zhejiangenis , 2016, 28(4): 595-600. (in Chinese with English abstract)
[22] 王少平, 孙乙铭, 姜艳, 等. 基于指纹图谱及柚皮素含量对铁皮石斛及伪品的鉴定[J]. 浙江农业学报, 2015, 27(12): 2199-2205.
WANG S P, SUN Y M, JIANG Y, et al. Discrimination of Dendrobium officinale and its counterfeits based on naringenin content determination and fingerprints technoly[J]. Acta Agriculturae Zhejiangenis , 2015, 27(12): 2199-2205.
[23] 李子唯, 杨野, 崔秀明, 等. 三七对镉胁迫的生理响应及富集特性研究[J]. 中国中药杂志, 2015, 40(15): 2903-2908.
LI Z W, YANG Y, CUI X M, et al. Physiological response and bioaccumulation of Panax notoginseng to cadimium under hydroponic[J]. China Journal of Chinese Materia Media , 2015, 40(15): 2903-2908. (in Chinese with English abstract)
[24] 李丽锋, 卢佳, 苏芳莉. 镉(Cd)胁迫对芦苇叶片SOD、POD活性影响及动力学分析[J]. 沈阳农业大学学报, 2014, 45(3): 326-330.
LI L F, LU J, SU F L. Effects of cadmium stress on SOD/POD activities in reed leaf and their dynamic models[J]. Journal of Shenyang Agricultural University , 2014, 45(3): 326-330. (in Chinese with English abstract)

镉胁迫对铁皮石斛叶片抗氧化酶活性的影响及动力学分析

Kinetic analysis and antioxidant activity of Dendrobium officinale leaves under cadmium stress

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