辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征

doi:10.3969/j.issn.1004-1524.2017.09.11

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1498-1505.DOI: 10.3969/j.issn.1004-1524.2017.09.11

辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征

庞欣¹, 程远², 郭勤卫³, 郑佳秋⁴, 万红建^{2, *}

1.苏州农业职业技术学院,江苏苏州 215008;
2.浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地,浙江省农业科学院蔬菜研究所,浙江杭州 310021;
3.衢州市农业科学研究院,浙江衢州 324000;
4.江苏沿海地区农业科学研究所,江苏盐城 224002

收稿日期:2017-01-13 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 万红建,E-mail:wanhongjian@sina.com
作者简介:庞欣(1985—),女,黑龙江哈尔滨人,博士,讲师,从事蔬菜遗传育种与分子生物学研究。E-mail:pxtracy916@163.com
基金资助:
江苏省自然科学基金青年基金项目(BK20140277); 苏州市科技发展计划项目应用基础研究(SYN201418); 苏州农业职业技术学院青年教师科研能力提升计划资助项目(PPN201308); 浙江省自然科学基金(LQ15C150002); 国家自然科学基金 (31501749); 国家自然科学基金青年人才培养计划 (2015R23R08E07, 2015R23R08E09); 国家重点实验室开放项目 (2010DS700124-KF1517)

Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper

PANG Xin¹, CHENG Yuan², GUO Qinwei³, ZHENG Jiaqiu⁴, WAN Hongjian^{2, *}

1. Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Quzhou Academy of Agricultural Sciences, Quzhou 324000, China;
4. Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, China

Received:2017-01-13 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 紫色酸性磷酸酶(purple acid phosphatases,PAPs)属于金属磷酸酯酶家族,参与了植物大量的生理反应,特别是磷素的吸收与利用。本研究利用已经发表的辣椒基因组数据,采用同源比对方法对辣椒PAP基因家族进行鉴定与表达分析。通过鉴定发现辣椒基因组中至少含有25个PAP基因成员,氨基酸序列长度在264~639 aa,具有1~12个内含子,不均匀地分布在12条染色体上,仅发现1对基因以串联重复的形式存在。系统发育关系表明,辣椒、拟南芥和水稻PAP基因家族成员可以分为3个家族,8个亚家族,每个家族成员的数目是变异的,而且存在6对直系同源基因和20对旁系同源基因,这表明PAP基因家族的存在早于辣椒、拟南芥和水稻的分化。基于RNA-seq数据库和qRT-PCR方法,对辣椒PAP基因的组织表达及果实发育的不同时期进行分析,结果表明PAP家族基因具有组织表达特异性和发育时期表达特异性。

关键词: 辣椒, 紫色酸性磷酸酶, 基因家族, 生物信息学, 表达分析

Abstract: Purple acid phosphatases (PAPs) are members of the metallo-phosphoesterases involved in a variety of physiological functions, especially phosphate acquisition in plants. In this paper, genome-wide identification and analysis of PAP gene family members in pepper were performed with homologues alignment method. These results found that the whole pepper genome contains at least 25 PAP genes with protein sequence length varying from 264 to 639 aa. Number of introns ranged from 1 to 12. They are distributed unevenly on 12 chromosomes, only had one tandem duplication. Phylogenetic relationships showed that PAP genes from three plants species (pepper, Arabidopsis and rice) can be separated into 3 groups, 8 subgroups, and the number of each group is variable. Six and twenty pairs of orthologous and homologous genes were found, indicating that the diversity of PAP genes occurred before the split of pepper, Arabidopsis and rice. Based on the RNA-seq database and qRT-PCR method, the expression analysis of different tissues and fruit development stages indicated that these genes had tissue and development stages specificity.

Key words: pepper, purple acid phosphatases, gene family, bioinformatics, expression analysis

中图分类号:

S641.3

庞欣, 程远, 郭勤卫, 郑佳秋, 万红建. 辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征[J]. 浙江农业学报, 2017, 29(9): 1498-1505.

PANG Xin, CHENG Yuan, GUO Qinwei, ZHENG Jiaqiu, WAN Hongjian. Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper[J]. , 2017, 29(9): 1498-1505.

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辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征

Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper

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