观赏石榴种质资源遗传多样性的ISSR分析

doi:10.3969/j.issn.1004-1524.2018.02.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (2): 236-241.DOI: 10.3969/j.issn.1004-1524.2018.02.08

观赏石榴种质资源遗传多样性的ISSR分析

王庆军¹, 马丽², 郝兆祥¹, 罗华¹, 毕润霞¹, 赵丽娜¹, 侯乐峰^{1, *}

1.枣庄市石榴研究中心,山东枣庄 277300;
2.枣庄学院,山东枣庄 277160

收稿日期:2017-06-30 出版日期:2018-02-20 发布日期:2018-02-11
通讯作者: 侯乐峰,E-mail:houlefeng@126.com
作者简介:王庆军(1987—),男,山东聊城人,硕士,助理农艺师,主要从事石榴种质资源研究。E-mail:651292186@qq.com
基金资助:
国家林木种苗工程(发改投资[2010]2167号); 山东省重点研发计划(2016GNC110002); 山东省农业科技发展资金项目(2015); 山东省高等学校科技计划项目(J14LF02); 枣庄英才集聚工程(枣政办字[2016]23号); 枣庄市科技计划项目(2016NS09)

Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers

WANG Qingjun¹, MA Li², HAO Zhaoxiang¹, LUO Hua¹, BI Runxia¹, ZHAO Li'na¹, HOU Lefeng^{1, *}

1.Zaozhuang Pomegranate Research Center, Zaozhuang 277300,China;
2. Zaozhuang University, Zaozhuang 277160,China

Received:2017-06-30 Online:2018-02-20 Published:2018-02-11

摘要/Abstract

摘要： 为了解观赏石榴种质资源遗传多样性及其亲缘关系,对35个观赏石榴品种利用ISSR标记技术进行遗传关系分析。筛选出多态性高的8条ISSR引物,共扩增出168个DNA位点,其中多态性DNA位点有158个,多态性位点比率(PPB)为94.05%。平均观察等位基因数(Na)为1.940 5,平均有效等位基因数(Ne)为1.678 5,平均Nei's基因多样性指数(He)为0.379 9,平均Shannon信息指数(I)为0.553 0。品种间遗传相似系数(GS)介于0.416 7~0.922 6,表明观赏石榴品种间存在比较丰富的遗传多样性。利用UPGMA法构建分子树状图,以遗传相似性系数(GS)0.668为阈值,将35个观赏石榴品种分为6个类群。

关键词: 观赏石榴, 遗传多样性, ISSR

Abstract: In order to investigate the germplasm genetic diversity and genetic relationship of ornamental pomegranate (Punica granatum L.), the genetic relationships of 35 ornamental pomegranate cultivars were evaluated by ISSR markers. Eight ISSR primers with good polymorphism were screened out. A total of 168 bands were amplified from 8 ISSR primers, of which 158 bands were polymorphic loci. The percentage of polymorphic band (PPB) was 94.05%, the number of alleles (Na) was 1.940 5, the effective number of alleles (Ne) was 1.678 5, Nei's gene diversity (He) was 0.379 9 and Shannon's information index (I) was 0.553 0. The genetic similarities coefficient (GS) between cultivars ranged from 0.416 7 to 0.922 6, suggesting ornamental pomegranate cultivars had abundant genetic diversity. Thirty-five ornamental pomegranate cultivars were divided into 6 groups at GS of 0.668 based on UPGMA method.

Key words: ornamental pomegranate (Punica granatum L.), genetic diversity, ISSR

中图分类号:

S665.4

王庆军, 马丽, 郝兆祥, 罗华, 毕润霞, 赵丽娜, 侯乐峰. 观赏石榴种质资源遗传多样性的ISSR分析[J]. 浙江农业学报, 2018, 30(2): 236-241.

WANG Qingjun, MA Li, HAO Zhaoxiang, LUO Hua, BI Runxia, ZHAO Li'na, HOU Lefeng. Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers[J]. , 2018, 30(2): 236-241.

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观赏石榴种质资源遗传多样性的ISSR分析

Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers

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