施氮量对烤烟氮代谢关键酶基因表达及代谢产物的影响

doi:10.3969/j.issn.1004-1524.2018.03.15

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (3): 454-460.DOI: 10.3969/j.issn.1004-1524.2018.03.15

施氮量对烤烟氮代谢关键酶基因表达及代谢产物的影响

鲁黎明¹, 曾孝敏¹, 顾会战², 张启莉², 喻晓², 王栋², 何佶弦², 李立芹^1,*

1.四川农业大学农学院,四川成都 611130;
2.四川省烟草公司广元市公司,四川广元 628017

收稿日期:2017-07-13 出版日期:2018-03-20 发布日期:2018-03-21
通讯作者: *李立芹,E-mail: lilq88@126.com
作者简介:鲁黎明(1965—),男,河南正阳人,博士,副教授,研究方向为烟叶品质。E-mail: luliming@sicau.edu.cn
基金资助:
四川省烟草公司科技项目(201302006);广元市烟草公司科技项目(广元市特色烤烟品种遴选和适应性研究)

Effects of nitrogen application on key enzymes gene expression and yield of nitrogen metabolism in flue-cured tobacco

LU Liming¹, ZENG Xiaomin¹, GU Huizhan², ZHANG Qili², YU Xiao², WANG Dong², HE Jixian², LI Liqin^1,*

1. College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China;
2. Sichuan Tobacco Company Guangyuan Division, Guangyuan 628017, China

Received:2017-07-13 Online:2018-03-20 Published:2018-03-21

摘要/Abstract

摘要： 为了探寻供氮水平对烤烟氮代谢关键基因表达与含氮化合物含量的影响,进行供氮水平及品种的两因素田间试验,分别在移栽后45、60、80 d测定鲜烟叶硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)基因表达量,以及烟叶总氮、烟碱和可溶性蛋白质的含量。结果显示,3个氮代谢关键基因的表达均受施氮量影响。栽后80 d,高氮处理(120 kg·hm^-2)的烟株可溶性蛋白含量显著(P<0.05)高于低氮处理(90 kg·hm^-2)。栽后45 d,NR基因表达量与烟碱含量呈显著(P<0.05)负相关,与可溶性蛋白含量呈极显著(P<0.01)正相关;栽后60 d,GS基因表达量与烟碱含量呈显著(P<0.05)负相关。施氮水平对烤烟氮代谢关键酶基因表达及氮代谢产物含量有显著影响,可通过调整施氮量来调控烟叶含氮化合物含量。

关键词: 烤烟, 氮代谢, 基因表达, 施氮量, 品质

Abstract: In order to investigate the effects of nitrogen supply levels on nitrogen metabolism, key gene expression and nitrogen compounds content in flue-cured tobacco, a two-factor field experiment of nitrogen supply level and tobacco variety was conducted. The gene expressions of nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT) gene, as well as total nitrogen, nicotine and soluble protein contents in flue-cured tobacco leaves were measured at 45, 60 and 80 d after planting, respectively. It was shown that the expression of these three key nitrogen metabolism genes were influenced by nitrogen supply. Eighty days after planting, soluble protein contents in tobacco with high N (120 kg·hm^-2) was significantly (P<0.05) higher than that with low N (90 kg·hm^-2). Correlation analysis showed that NR gene expression was significantly (P<0.05) negatively correlated with nicotine content, and was significantly (P<0.01) positively correlated with soluble protein content in tobacco at 45 d after planting. GS gene expression and nicotine content showed a significantly (P<0.05) negative correlation at 60 d after planting. These results suggested that the nitrogen application level had significant effect on key gene expression in nitrogen metabolism of flue-cured tobacco, and the content of nitrogenous compounds in tobacco leaves could be regulated by adjusting the application amount of nitrogen.

Key words: flue-cured tobacco, nitrogen metabolism, gene expression, nitrogen application amount, quality

中图分类号:

S571

鲁黎明, 曾孝敏, 顾会战, 张启莉, 喻晓, 王栋, 何佶弦, 李立芹. 施氮量对烤烟氮代谢关键酶基因表达及代谢产物的影响[J]. 浙江农业学报, 2018, 30(3): 454-460.

LU Liming, ZENG Xiaomin, GU Huizhan, ZHANG Qili, YU Xiao, WANG Dong, HE Jixian, LI Liqin. Effects of nitrogen application on key enzymes gene expression and yield of nitrogen metabolism in flue-cured tobacco[J]. , 2018, 30(3): 454-460.

参考文献

[1] 黄树永,陈良存.烟草碳氮代谢研究进展[J].河南农业科学,2005, 34(4): 8-11.
HUANG S Y, CHEN L C.Research advance on carbon and nitrogen metabolism in tobacco[J]. Journal of Henan Agriculture Science, 2005, 34(4): 8-11. (in Chinese with English abstract)
[2] 岳红宾. 不同氮素水平对烟草碳氮代谢关键酶活性的影响[J].中国烟草科学, 2007, 28(1):18-20, 24.
YUE H B.Effects of various nitrogen levels on key enzymes activeness of flue-cured tobacco leaves in carbon and nitrogen metabolism[J]. Chinese Tobacco Science, 2007, 28(1):18-20, 24. (in Chinese with English abstract)
[3] 许晨曦,刘国顺,李向阳,等.品种与施氮量互作对烟草碳氮代谢关键酶的影响[J].江苏农业科学,2012, 40(3):83-85.
XU C X, LIU G S, LI X Y, et al.Effects of variety and amount of nitrogen on key enzymes of carbon and nitrogen metabolism in tobacco[J]. Jiangsu Agriculture Science, 2012, 40(3):83-85. (in Chinese)
[4] 李洪臣,杨志晓,武云杰,等.氮肥用量和施用方式对烟草中部叶碳氮代谢的影响[J]. 江苏农业科学, 2013, 41(3):65-68.
LI H C, YANG Z X, WU Y J, et al.Effects of amount and mode of nitrogen fertilizer application on carbon and nitrogen in middle part of tobacco[J]. Jiangsu Agriculture Science, 2013, 41(3):65-68. (in Chinese)
[5] 王红丽,杨惠娟, 苏菲, 等. 氮用量对烤烟成熟期叶片碳氮代谢及萜类代谢相关基因表达的影响[J]. 中国烟草学报, 2014, 20(5):116-120.
WANG H L, YANG H J, SU F, et al.Effects of nitrogen on expression of key genes related to carbon/nitrogen metabolism and terpenoid metabolism in matruing flue-cured tobacco leaves[J]. Acta Tabacaria Sinica, 2014, 20(5):116-120. (in Chinese with English abstract)
[6] 刘维智. 烤烟碳氮代谢对氮素形态的响应及NRT2.2的克隆[D]. 郑州:河南农业大学,2014.
LIU W Z.Carbon and nitrogen metabolism in response to nitrogen form and cloning of NRT2.2[D]. Zhegngzhou: Henan Agricultural University, 2014. (in Chinese with English abstract)
[7] 崔保伟,陆引罡,张振中,等.不同施氮量对烤烟生理特性及化学品质的影响[J].山地农业生物学报,2008, 27(5):377-381.
CUI B W, LU Y G, ZHANG Z Z, et al.Effect of nitrogen rates on the physiology characteristic and the chemistry quality of flue-cured tobacco[J]. Journal of Mountain Agriculture and Biology, 2008, 27(5): 377-381. (in Chinese with English abstract)
[8] 习向银,晁逢春,陈亚,等.不同施氮量对烤烟氮素和烟碱累积的影响[J].西南大学学报(自然科学版),2008, 30(5): 110-115.
XI X Y, CHAO F C, CHEN Y, et al.Effects of different nitrogen rates on the growth and nitrogen and nicotine accumulation in flue-cured tobacco[J]. Journal of Southwest University (Natural Science Edition), 2008, 30(5): 110-115.(in Chinese with English abstract)
[9] 解莹莹. 施肥和打顶对烤烟中主要含氮化合物含量的影响[D].郑州:河南农业大学, 2004.
XIE Y Y.Effects of fertilization and topping on the contents of major nitrogen compounds in flue cured tobacco[D]. Zhegngzhou: Henan Agricultural University, 2004. (in Chinese with English abstract)
[10] 邓云龙,孔光辉,武锦坤.云南烤烟中上部叶片含氮化合物代谢规律研究[J].云南大学学报(自然科学版),2001, 23(1): 65-70.
DENG Y L, KONG G H, WU J K.Research on the metabolism rules of nitrogenous compounds in flue-cured tobacco leaves in Yunnan[J]. Journal of Yunnan University (Natural Sciences), 2001, 23(1): 65-70. (in Chinese with English abstract)
[11] 李文卿,陈顺辉,江荣风,等.不同施氮量对烤烟总氮和烟碱积累的影响[J].中国烟草学报,2007, 13(4):31-35.
LI W Q, CHEN S H, JIANG R F, et al.Effects of nitrogenous fertilizer on total nitrogen and nicotine accumulation in flue-cured tobacco[J]. Acta Tabacaria Sinica, 2007, 13(4): 31-35. (in Chinese with English abstract)
[12] 王瑞新. 烟草化学[M].北京:中国农业出版社, 2003.
[13] 赵英永,戴云,崔秀明,等. 考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量[J]. 云南民族大学学报(自然科学版), 2006, 15(3):235-237.
ZHAO Y Y, DAI Y, CUI X M, et al.Determination of protein contents of Radix aconiti Kusnezoffii using coomassie brillant blue G-250 dye binding[J]. Journal of Yunnan Nationalities University (Natural Sciences Edition), 2006, 15(3): 235-237. (in Chinese with English abstract)
[14] 鲁黎明,杨铁钊. 烟草钾转运体基因 NtHAK1 的克隆及表达模式分析[J]. 核农学报,2011, 25(3):469-476.
LU L M, YANG T Z.Cloning and expression profile analysis of a putative potassium transporter gene NtHAK1 in tobacco[J]. Acta Agriculturae Nucleatae Sinica, 2011, 25(3):469-476. (in Chinese with English abstract)
[15] 樊武广, 王振海, 牛书金, 等. 不同氮源对烤烟碳氮代谢关键酶和化学成分的影响[J].江西农业学报, 2012, 24(10):50-52, 58.
FAN W G, WANG Z H, NIU S J, et al.Effects of different nitrogen sources on enzyme activity in carbon and nitrogen metabolism, and chemical composition in flue-cured tobacco[J]. Acta Agriculturae Jiangxi, 2012, 24(10):50-52, 58. (in Chinese with English abstract)
[16] 赵宪凤. 云南三地区烟草的代谢特征和差异分析[D]. 郑州:河南农业大学, 2012.
ZHAO X F.Metabolic characteristics and differences of tobacco in three areas of Yunnan[D]. Zhegngzhou: Henan Agricultural University, 2012. (in Chinese with English abstract)
[17] 张森,许自成,李京京,等. 烟草碳氮代谢及其调控技术研究进展[J]. 生物技术进展,2016, 6(5): 312-318.
ZHANG S, XU Z C, LI J J, et al.Advance on carbon and nitrogen metabolism and regulation of tobacco[J]. Current Biotechnology, 2016, 6(5): 312-318. (in Chinese with English abstract)
[18] 李玉潜,谢九生,谭中文.甘蔗叶片碳、氮代谢与产量、品质关系研究初探[J].中国农业科学,1995, 28(4):46-53.
LI Y Q, XIE J S, TAN Z W.Studies on the correlations among metabolisms of foliar carbohydrate and nitrogen, and yield and quality in sugarcane[J]. Scientia Agricultura Sinica, 1995, 28(4):46-53. (in Chinese with English abstract)
[19] 刘桃菊,朱冰,江绍琳,等. 施氮量对双季稻氮素吸收和产量的影响及其优化[J]. 浙江农业学报, 2014, 26(4): 1004-1009.
LIU T J, ZHU B, JIANG S L, et al.Effect of nitrogen rates on N uptake and use efficiency in double season rice and optimization of nitrogen application[J]. Acta Agriculturae Zhejiangensis, 2014, 26(4):1004-1009. (in Chinese with English abstract)
[20] 陈新红,韩正光,张安存,等. 不同施氮量与种植密度对水稻穗上不同部位结实特性的影响[J]. 浙江农业学报, 2014, 26(6):1578-1582.
CHEN X H, HAN Z G, ZHANG A C, et al.Analysis on rice grain filling characteristics in different spikelet positions under different nitrogen rates and planting densities[J].Acta Agriculturae Zhejiangensis, 2014, 26(6):1578-1582. (in Chinese with English abstract)
[21] 李雪利, 叶协锋, 顾建国,等. 土壤C/N比对烤烟碳氮代谢关键酶活性和烟叶品质影响的研究[J]. 中国烟草学报, 2011,17(3):32-36.
LI X L, YE X F, GU J G, et al.Effect of soil C/N ratio on activity of key enzymes involved in carbon and nitrogen metabolism and quality of flue-cured tobacco leaves[J]. Acta Tabacaria Sinica, 2011,17(3):32-36.(in Chinese with English abstract)
[22] 李春俭,张福锁,李文卿,等.我国烤烟生产中的氮素管理及其与烟叶品质的关系[J]. 植物营养与肥料学报,2007, 13(2):331-337.
LI C J, ZHANG F S, LI W Q, et al.Nitrogen management and its relation to leaf quality in production of flue-cured tobacco in China[J]. Plant Nutrition and Fertilizer Science, 2007, 13(2):331-337.(in Chinese with English abstract)
[23] 连文力. 施氮量对烟叶成熟期重要物质代谢及基因表达的影响研究[D]. 郑州:河南农业大学,2016.
LIAN W L.The effect on the amount of nitrogen on important material metabolism and gene expression of tobacco in maturity[D]. Zhegngzhou: Henan Agricultural University, 2016. (in Chinese with English abstract)
[24] 朱方旭. 水稻碳氮代谢关键酶基因表达及产量和品质性状对氮素营养的响应[D]. 哈尔滨:东北农业大学,2016.
ZHU F X.Response of rice carbon-nitrogen metabolism key enzyme gene expression and the yield and quality to nitrogen at filling stage[D]. Harbin: Northeast Agriculture University, 2016. (in Chinese with English abstract)
[25] 黄成能,卢晓鹏,肖玉明,等. 施氮对椪柑叶片氮同化叶绿素合成关键酶基因表达的影响[J]. 果树学报, 2014, 31(1):7-12.
HUANG C N, LU X P, XIAO Y M, et al.Effect of nitrogen application on expression of key enzyme genes in pathway of Ponkan leaf nitrogen assimilation and chlorophyll biosynthesis[J]. Journal of Fruit Science, 2014, 31(1):7-12. (in Chinese with English abstract)

施氮量对烤烟氮代谢关键酶基因表达及代谢产物的影响

Effects of nitrogen application on key enzymes gene expression and yield of nitrogen metabolism in flue-cured tobacco

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