浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 537-547.DOI: 10.3969/j.issn.1004-1524.2018.04.03

• 动物科学 • 上一篇    下一篇

黄精多糖的提取及其对CCl4致大鼠肝损伤的保护作用

韩春杨, 杨明川, 杨孜生, 冯伉梨, 刘翠艳*   

  1. 安徽农业大学 动物科技学院,安徽 合肥 230036
  • 收稿日期:2017-08-14 出版日期:2018-04-20 发布日期:2018-04-19
  • 通讯作者: 刘翠艳,E-mail:cyliu@ahau.edu.cn
  • 作者简介:韩春杨(1972—),男,河北衡水人,博士,副教授,主要从事中药药理学及小动物疾病研究。E-mail:luckyhcy@ahau.edu.cn
  • 基金资助:
    国家自然科学基金(31772786); 2017年安徽农业大学大学生创新创业训练项目(XJDC2016068)

Study on extraction of Polygonatum sibiricum polysaccharides and its protective effect on CCl4-induced acute liver injury in rats

HAN Chunyang, YANG Mingchuan, YANG Zisheng, FENG Kangli, LIU Cuiyan*   

  1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:2017-08-14 Online:2018-04-20 Published:2018-04-19

摘要: 对黄精多糖(PSP)的最佳提取工艺及其对CCl4诱导的大鼠肝损伤的保护作用进行初步研究。首先,对影响PSP提取率的3个主要因素(液料比、时间和温度)进行单因素试验;然后,以PSP提取率为响应值,利用响应面分析法对其提取工艺进行优化;最后,将提取的PSP用于CCl4诱导的大鼠肝损伤保护实验。动物试验共分5组:对照组,模型组,PSP低、中、高剂量用药组(75、150、300 mg·kg-1),3个用药组大鼠灌胃不同剂量的PSP,对照组和模型组灌胃等量的蒸馏水,连续7 d。末次灌药1 h后,模型组和用药组大鼠按1 mL·kg-1的剂量腹腔注射50% CCl4油溶液,对照组注射等量油溶剂,注射24 h后处死大鼠,采集血液和肝脏,观察肝脏病理变化及组织学变化,检测血清中丙氨酸转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性及直接胆红素(DBIL)、总胆红素(TBIL)含量。结果显示,PSP的最佳提取工艺条件为:液料比19.13:1,时间3.49 h,温度73.33 ℃。在此条件下,PSP的实际提取率为10.107%。动物实验中,与模型组相比,中、高剂量的PSP能显著降低(P<0.05)大鼠血清中ALT、AST、ALP活性及DBIL、TBIL含量,各剂量PSP均能不同程度地减轻大鼠肝脏病理学和组织学病变,以高剂量PSP效果最好。可见,黄精多糖对CCl4诱导的大鼠肝损伤有较好的保护作用。

关键词: 黄精多糖, 提取, 大鼠, 肝损伤

Abstract: The present study was designed to investigate optimum extraction process of Polygonatum sibiricum polysaccharides (PSP) and explore its protective effect on carbon tetrachloride (CCl4) induced liver injury in rats. Firstly, single factor experiment was carried out on the three main factors, including the ratio of liquid to material, time and temperature. Then, taking PSP extraction rate as response value, the extraction process was optimized by response surface analysis. Finally, the PSP was used to protect against CCl4-induced liver injury in rats. The experiment rats were randomly divided into 5 groups: control group, model group, low dose, middle dose and high dose (75, 150, 300 mg·kg-1) of PSP groups. The rats of PSP groups were administered with different doses of PSP by gavage. Control group and model group were fed with the same amount of distilled water. All rats were fed for 7 d. One hour after the last administration, the rats in the model group and PSP groups were injected intraperitoneally with 50% CCl4 oil solution at the dose of 1 mL·kg-1,and rats in the control group were received the same amount of soybean oil. Blood and liver samples were collected 24 h later after the last administration to test the pathological and histological changes of liver, the activities of ALT (alanine aminotransferase), AST (aspartate aminotransferase), ALP (alkaline phosphatase;) and the content changes of DBIL (direct bilirubin), TBIL (total bilirubin). The results showed that the optimum extraction conditions of PSP were as follows: liquid material ratio was 19.13:1, extraction time was 3.49 h and temperature was 73.33 ℃. Under these conditions, the actual PSP extraction rate was 10.107%. Compared with the model group, the middle dose PSP group and high dose PSP group could significantly (P<0.05) reduce the activities of ALT, AST, ALP and the contents of DBIL, TBIL in serum of rats. All doses of PSP could alleviate the pathological and histological changes of liver in different degrees, and the effect of high dose of PSP was the best. These results indicated that PSP had protective effect on CCl4-induced liver injury in rats.

Key words: Polygonatum sibiricum polysaccharides, extraction, rat, liver injury

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