大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较

doi:10.3969/j.issn.1004-1524.2018.08.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (8): 1328-1335.DOI: 10.3969/j.issn.1004-1524.2018.08.08

大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较

马晓平¹, 杨秋霞¹, 俞演¹, 李德生², 王承东², 凌珊珊², 古玉^3,*

1.四川农业大学动物医学院,四川成都611130;
2.中国大熊猫保护研究中心,四川雅安 625000;
3.四川农业大学生命科学学院,四川雅安 625014

收稿日期:2018-01-08 出版日期:2018-08-25 发布日期:2018-08-28
通讯作者: 古玉,E-mail: guyu632@qq.com
作者简介:马晓平(1976—),男,土家族,重庆人,博士,副教授,从事临床兽医学教学和科研工作。E-mail: mxp886@sina.com.cn
基金资助:
大熊猫国际资金项目(AD1415); 四川省科技厅基础研究计划(2013JY0175)

Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

MA Xiaoping¹, YANG Qiuxia¹, YU Yan¹, LI Desheng², WANG Chengdong², LING Shanshan², GU Yu^3,*

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. China Conservation and Research Center for Giant Panda, Ya'an 625000, China;
3. College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China;

Received:2018-01-08 Online:2018-08-25 Published:2018-08-28

摘要/Abstract

摘要： 为探明枝孢样枝孢霉野生株Z20与突变株Zt在生物学特性及药物敏感上的差异,将枝孢样枝孢霉Z20和Zt于PDA培养基上连续培养7 d,每天观察菌落形态及长势。将菌株Z20和Zt在沙氏液基上培养4 d后用透射电镜对其超微结构进行观察。采用硫酸-苯酚法测定枝孢样枝孢霉Z20和Zt的菌丝体多糖含量。运用96孔板微量稀释法测定5种常见抗真菌药物对枝孢样枝孢霉Z20和Zt的最小抑菌浓度。结果表明:在PDA培养基上菌株Zt的生长速率高于Z20,菌落颜色较浅,孢子数量减少。在透射电镜下,野生株Z20的菌丝壁厚度和菌丝宽度显著大于突变株Zt,且菌丝的含糖量也显著高于突变株Zt。药敏试验中,除灰黄霉素外,枝孢样枝孢霉Zt对5种药物的敏感性均高于Z20。

关键词: 枝孢样枝孢霉, 生物学特性, 最小抑菌浓度, 药敏实验

Abstract: In order to explore the difference of biological characteristics and drug sensitivity of Cladosporium cladosporioides (Z20) and the mutant strain (Zt), Z20 and Zt were continuously incubated on PDA medium for 7 d, and the colony morphology and growth information were observed and recorded every day. The ultrastructure of Z20 and Zt were observed under the transmission electron microscope. The polysaccharide contents of Z20 and Zt were detected by sulfuric acid-phenol method. The minimum inhibitory concentrations (MICs) of five commonly used antifungal agents against Cladosporium cladosporioides Z20 and Zt were determined by 96-well plate microdilution method. The results showed as follows: The growth rate of Zt was higher than Z20, the colour of colony was shallow, but the number of spores decreased on PDA by continuously incubate. Under the transmission electron microscope, the mycelial wall thickness and mycelial width of wild strain Z20 were significantly greater than those of mutant strain Zt, and sugar content of mycelium was also significantly higher than that of mutant strain Zt. Antifungal susceptibility test showed that except griseofulvin, the sensitivity of mutant strain Zt to five drugs were higher than that of the Z20.

Key words: Cladosporium cladosporioides, biological characteristics, minimal inhibitory concentration, drug sensitivity test

中图分类号:

马晓平, 杨秋霞, 俞演, 李德生, 王承东, 凌珊珊, 古玉. 大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较[J]. 浙江农业学报, 2018, 30(8): 1328-1335.

MA Xiaoping, YANG Qiuxia, YU Yan, LI Desheng, WANG Chengdong, LING Shanshan, GU Yu. Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)[J]. , 2018, 30(8): 1328-1335.

参考文献

[1] 桑红,邓德权,何威. 枝孢样枝孢霉的研究概况[J]. 中国真菌学杂志,2010, 5(1):57-60.
SANG H, DENG D Q, HE W.Research survey of Cladosporium cladosporioides[J]. Chinse Journal of Mycology, 2010, 5(1):57-60. (in Chinese)
[2] GUGNANI H C, SOOD N, SINGH B, et al.Case report. Subcutaneous phaeohyphomycosis due to Cladosporium cladosporioides[J]. Mycoses, 2000, 43(1/2):85-87.
[3] POLACK F M, SIVERIO C, BRESKY R H.Corneal chromomycosis: double infection by Phialophora verrucosa(Medlar) and Cladosporium cladosporioides (Frescenius)[J]. Annals of Ophthalmology, 1976, 8(2):139-144.
[4] POUTAHIDIS T, ANGELOPOULOU K, KARAMANAVI E, et al.Mycotic encephalitis and nephritis in a dog due to infection with Cladosporium cladosporioides[J]. Journal of Comparative Pathology, 2009, 140(1):59-63.
[5] 马晓平,古玉,刘小敏,等. 大熊猫源枝孢样枝孢霉的分离鉴定[J]. 中国兽医科学,2013(12):1217-1223.
MA X P, GU Y, LIU X M, et al.Isolation and identification of Cladosporium cladosporioides from Ailuropoda melanoleuca[J]. Chinese Veterinary Science, 2013(12):1217-1223. (in Chinese with English abstract)
[6] BOUZIANE H, LATGE J P, LELONG M.Immunochemical comparison of the allergenic potency of spores and mycelium of Cladosporium cladosporioides extracts by a nitrocellulose electroblotting technique[J]. Allergologia et Immunopathologia, 2006, 34(2):64-69.
[7] 马晓平, 刘艳芳, 古玉,等. 枝孢样枝孢霉弱毒株的微波诱变结合亚硝基胍诱变选育[J]. 中国兽医科学,2015 (9):923-929.
MA X P, LIU Y F, GU Y, et al.Breeding of lentogenic Cladosporium cladosporioides mutated by microwave and nitrosoguanidine[J]. Chinese Veterinary Science, 2015 (9):923-929. (in Chinese with English abstract)
[8] 燕丽, 刘伟, 韩立华,等. 犬小孢子菌微量液基稀释法抗真菌药敏试验的方法学研究[J].首都医科大学学报, 2006, 27(6):750-753.
YAN L, LIU W, HAN L H,et al.Research about the broth microdilution antifungal susceptibility testing condition for 182 Microsporum canis[J]. Journal of Capital University of Medical Sciences, 2006, 27(6):750-753. (in Chinese with English abstract)
[9] 王端礼. 医学真菌学:实验室检验指南 [M]. 北京:人民卫生出版社,2005.
[10] 桑红,何威,姜少军,等. 枝孢样枝孢霉与卡氏枝孢霉形态超微结构的比较研究[J]. 医学研究生学报,2008,21(4):356-359.
SANG H, HE W, JIANG S J, et al.A comparative study on the ultrastructural difference between Cladosporium cladosporioides and Cladosporium carrioii[J]. Journal of Medical Postgraduates, 2008, 21(4):356-359. (in Chinese with English abstract)
[11] 姚自奇,兰进. 杏鲍菇不同菌株生物学特性的研究[J]. 食用菌学报,2005,12(2):16-20.
YAO Z Q, LAN J.Studies on the biological characteristics of different Pleurotus eryngii strains[J]. Acta Edulis Fungi, 2005, 12(2):16-20. (in Chinese with English abstract)
[12] TSAI H F, CHNG Y C, WASHBURN R G, et al.The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence[J]. Journal of Bacteriology, 1998, 180(12):3031-3038.
[13] LANGFELDER K, JAHN B, GEHRINGER H, et al.Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence[J]. Medical Microbiology and Immunology, 1998, 187(2):79-89.
[14] JAHN B, BOUKHALLOUK F, LOTZ J, et al.Interaction of human phagocytes with pigmentless Aspergillus Conidia[J]. Infection & Immunity, 2000, 68(6):3736-3739.
[15] 张宇. 用比较蛋白质组学研究烟曲霉的致病机制[D]. 长春:吉林大学,2010.
ZHANG Y.Comparative proteomics analysis on pathogenic mechanism of Aspergillus fumigatus[D]. Changchun: Jilin University, 2010. (in Chinese with English abstract)
[16] 许启泰,张瑜,杜钢军,等. 嗜酸乳杆菌胞外多糖对小鼠免疫功能的影响[J]. 中国药理学通报,2004,20(12):1401-1403.
XU Q T, ZHANG Y, DU G J, et al.Effect of lacidophilus exopolysaccharides on immunity[J]. Chinese Pharmacological Bulletin, 2004, 20(12):1401-1403. (in Chinese with English abstract)
[17] 宿红艳,王磊,高兴喜,等. 白灵菇不同菌株生物学特性的研究[J]. 食品科学,2008,29(7):256-259.
SU H Y, WANG L, GAO X X, et al.Study on biological characteristics of different Pleutotus nerbrodensis strains[J]. Food Science, 2008, 29(7):256-259. (in Chinese with English abstract)
[18] 张长青,张建民,赵明敏,等. 培养基配方对白灵菇菌丝生长的影响[J]. 贵州农业科学,2007,35(1):18-20.
ZHANG C Q, ZHANG J M, ZHAO M M, et al.Effects of different medium formula on hyphal growth of Pleutotus nerbrodensis[J]. Guizhou Agricultural Sciences, 2007, 35(1):18-20. (in Chinese with English abstract)
[19] 黄建华, 范文秀. 白灵菇多糖的提取及含量的测定[J]. 光谱实验室,2005,22(5):1014-1016.
HUANG J H, FAN W X.Abstraction and content measurement of polysaccharide in Pleurotus nebrodenisis[J]. Chinese Journal of Spectroscopy Laboratory, 2005, 22(5):1014-1016. (in Chinese with English abstract)
[20] 孙志坚,李若瑜,万喆,等. 产孢丝状真菌药敏试验检测暗色真菌对九种药物的敏感性[J]. 中华皮肤科杂志,2004,37(4):197-199.
SUN Z J,LI R Y,WAN Z,et al.Studies on susceptibility of nine antifungal agents against dematiaceous fungi by NCCLS M38-P method[J]. Chinese Journal of Dermatology, 2004, 37(4):197-199. (in Chinese with English abstract)
[21] 李厚敏, 刘伟, 李若瑜. 美国临床实验室标准化委员会2003年版产孢丝状真菌药敏试验方案简介[J]. 中华检验医学杂志,2005,28(1):121-123.
LI H M,LIU W,LI R Y.The brief introduction of drug sensitive of spore filamentous fungi that is the standardization committee of the American clinical laboratory (2003 version)[J]. Chinese Journal of Laboratory Medicine, 2005, 28(1):121-123. (in Chinese)
[22] 孙志坚,李若瑜,李东明,等. 产孢丝状真菌NCCLS药敏试验方法的应用[J]. 中华检验医学杂志,2001,24(1):43-44.
SUN Z J, LI R Y, LI D M, et al.Application of NCCLS drug susceptibility test method for spore filamentous fungi[J]. Chinese Journal of Laboratory Medicine, 2001, 24(1):43-44. (in Chinese)
[23] 李洪萍, 李洪梅. 伊曲康唑与氟康唑治疗马拉色菌毛囊炎疗效和安全性观察[J]. 临床医药文献杂志, 2017, 4(69):13615.
LI H P,LI H M.Efficacy and safety of itraconazole and fluconazole in the treatment of malassezia folliculitis[J]. Journal of Clinical Medical, 2017, 4(69):13615.(in Chinese)
[24] 李艳伟. 克霉唑联合伊曲康唑治疗霉菌性阴道炎的疗效观察[J]. 中国民康医学, 2017,29(18):43-44.
LI Y W.Effect of Clotrimazole and Itraconazole in the treatment of fungal vaginitis[J]. Medical Journal of Chinese People's Health, 2017, 29(18):43-44.(in Chinese)
[25] 陈昭燕,占美,田方圆,等. 伊曲康唑治疗口腔念珠菌病安全性与有效性的系统评价[J]. 中国抗生素杂志, 2018, 43(1):96-102.
CHEN S Y,ZHAN M,TIAN F Y, et al.Efficacy and safety of itraconazole in the treatment of oral candidiasis: a systematic review[J].Chinese Journal of Antibiotics, 2018, 43(1):1-7.(in Chinese with English abstract)
[26] 吕佩珊. 伊曲康唑和盐酸特比萘芬序贯法治疗甲真菌病的效果及安全性[J]. 中国实用医药,2017,12(1): 105-107.
LYU P S.Effect and security of sequential therapy with itraconazole and terbinafine hydrochloride in the treatment of onychomycosis[J]. China Practical Medicine, 2017, 12(1):105-107.(in Chinese with English abstract)

大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较

Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt)

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