青海省马铃薯黑胫病病原菌的鉴定

doi:10.3969/j.issn.1004-1524.2018.08.14

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (8): 1369-1375.DOI: 10.3969/j.issn.1004-1524.2018.08.14

青海省马铃薯黑胫病病原菌的鉴定

王信, 程亮, 王亚艺, 高旭升, 李松龄^*

青海大学农林科学院/省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810016

收稿日期:2017-08-07 出版日期:2018-08-25 发布日期:2018-08-28
通讯作者: 李松龄,E-mail: 465883114@qq.com
作者简介:王信(1975—),女,河南商丘人,硕士,副研究员,研究方向为植物病理学。E-mail: wangx710@163.com
基金资助:
青海省科技计划项目基础研究计划(2015-ZJ-705); 青海大学中青年科研基金(2014-QNY-7,2015-QNY-2)

Identification of blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai

WANG Xin, CHENG Liang, WANG Yayi, GAO Xusheng, LI Songling^*

Qinghai Academy of Agriculture and Forestry Sciences, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China

Received:2017-08-07 Online:2018-08-25 Published:2018-08-28

摘要/Abstract

摘要： 为明确引起青海省马铃薯黑胫病的病原菌,从青海省不同地区马铃薯黑胫病病样分离病原菌,研究病原菌的形态学特征、致病性、生理生化特性,并结合16S rRNA序列进行病原学鉴定。结果表明:引起马铃薯黑胫病的5个菌株均为黒腐果胶杆菌(Pectobacterium atrosepticum),来源不同的菌株接种马铃薯后致病力有差异;其中,3株强致病力菌株主要来自于乐都、湟中和大通,2株中等致病力菌株来自于民和和湟源。综上,黒腐果胶杆菌是引起青海省马铃薯黑胫病的病原菌。

关键词: 马铃薯, 黒腐果胶杆菌, 表型鉴定, 分子鉴定, 致病力

Abstract: The causal pathogen causing blackleg of Solanum tuberosum L. was observed in the plantation in Qinghai Province in recent years. Five strains isolaed from five counties in Qinghai were characterized by morphological, biochemical and physiological methods, and analyses of 16S rRNA sequence as well as Pa subspecies geneome. The results indicated that all of the five strains were pathogenic to potato and caused blackleg of potato, suggesting that Pectobacterium atrosepticum was the blackleg pathogens on potato in Qinghai. The pathogenicity tests of the bacterial strains showed significant difference among the species isolated from different regions in Qinghai. Three highly pathogenic strains came from Ledu, Huangzhong and Datong, two moderately pathogenic strains mainly came from Minhe and Huangyuan. Pectobacterium atrosepticum was the blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai Province.

Key words: Solanum tuberosum L., Pectobacterium atrosepticum, phenotype identification, molecular identification, pathogenicity

中图分类号:

S432.1

王信, 程亮, 王亚艺, 高旭升, 李松龄. 青海省马铃薯黑胫病病原菌的鉴定[J]. 浙江农业学报, 2018, 30(8): 1369-1375.

WANG Xin, CHENG Liang, WANG Yayi, GAO Xusheng, LI Songling. Identification of blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai[J]. , 2018, 30(8): 1369-1375.

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青海省马铃薯黑胫病病原菌的鉴定

Identification of blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai

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