浙江农业学报 ›› 2019, Vol. 31 ›› Issue (7): 1029-1036.DOI: 10.3969/j.issn.1004-1524.2019.07.01

• 作物科学 • 上一篇    下一篇

利用SSR分析浙南地区甜玉米自交系的遗传多样性

岳高红, 潘彬荣*, 刘永安, 梅喜雪, 许立奎, 张宗宸, 周志辉   

  1. 浙南作物育种重点实验室/温州市农业科学研究院 作物研究所,浙江 温州 325006
  • 收稿日期:2019-01-14 出版日期:2019-07-25 发布日期:2019-08-07
  • 通讯作者: *潘彬荣,E-mail: wzpbrong@126.com
  • 作者简介:岳高红(1981—),男,山西临汾人,硕士,副教授,主要从事旱粮新品种选育与推广的科研工作。E-mail: 362840793@qq.com
  • 基金资助:
    浙江省农业新品种选育重大科技专项(2016C02050-9-4); 浙江省教育厅一般科研项目(Y201533916); 温州市种子种苗科技创新专项(Z20170003)

Genetic diversity analysis of sweet maize inbred lines in Southern Zhejiang by SSR markers

YUE Gaohong, PAN Binrong*, LIU Yongan, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui   

  1. Southern Zhejiang Key Laboratory of Crop Breeding, Crop Research Institute of Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China
  • Received:2019-01-14 Online:2019-07-25 Published:2019-08-07

摘要: 为评价浙南地区甜玉米自交系的遗传多样性,指导甜玉米新品种的选育,本研究利用SSR分子标记对37份浙南地区甜玉米自交系进行了分析。结果显示,37个多态性SSR标记共检测到209个等位基因变异,平均为5.65个。SSR标记的多态性信息量PIC值为0.62~0.95,平均为0.82。自交系间的相似性系数为0.12~0.94,平均为0.40,说明这些甜玉米自交系之间亲缘关系较远,具有丰富的遗传多样性。UPGMA聚类分析将37份甜玉米自交系划分为3个类群,划分结果与其系谱及地理分布基本吻合。

关键词: 浙南地区, 甜玉米, 自交系, SSR标记, 遗传多样性

Abstract: To evaluate the genetic diversity of sweet maize inbred lines and to guide new sweet maize varieties breeding, 37 sweet maize inbred lines from Southern Zhejiang were analyzed using SSR molecular markers. The results showed that 209 alleles were detected by 37 polymorphic SSR markers, with an average of 5.65 alleles per SSR marker. Polymorphism information content (PIC) values of SSR markers were 0.62-0.95, with an average of 0.82 per primer pair. Similarity coefficients among inbred lines ranged from 0.12 to 0.94, with an average of 0.40, which suggested these sweet maize inbred lines had abundant genetic diversity. The 37 sweet maize inbred lines were classified into three groups by UPGMA method, which was consistent with the pedigree analysis and geographical distribution.

Key words: Southern Zhejiang, sweet maize, inbred lines, SSR marker, genetic diversity

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