烟草炭疽病原研究及拮抗链霉菌鉴定

doi:10.3969/j.issn.1004-1524.2019.08.15

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (8): 1337-1344.DOI: 10.3969/j.issn.1004-1524.2019.08.15

烟草炭疽病原研究及拮抗链霉菌鉴定

李继业¹, 王璟晶¹, 杨露¹, 彭丽娟^{2,3, *}

1.贵州大学农学院,贵州贵阳 550025;
2.贵州省烟草品质研究重点实验室,贵州贵阳 550025;
3.贵州大学烟草学院,贵州贵阳 550025

收稿日期:2019-01-22 出版日期:2019-08-25 发布日期:2019-08-30
通讯作者: 彭丽娟,E-mail: 296430006@qq.com
作者简介:李继业(1993-),男,黑龙江宾县人,硕士研究生,研究方向为植物病害生物防治。E-mail: 1305113140@qq.com
基金资助:
贵州省烟草公司遵义市公司项目(2017-06)

Identification of tobacco anthracnose and isolation of antagonistic Streptomyces

LI Jiye¹, WANG Jingjing¹, YANG Lu¹, PENG Lijuan^{2,3, *}

1. College of Agriculture, Guizhou University, Guiyang 550025, China;
2. Guizhou Key Laboratory for Tobacco Quality, Guiyang 550025, China;
3. College of Tobacco Science, Guizhou University, Guiyang 550025, China

Received:2019-01-22 Online:2019-08-25 Published:2019-08-30

摘要/Abstract

摘要： 从贵州省绥阳县分离到1株烟草炭疽病病原,为明确其分类学地位,寻找合适的生防链霉菌株,对其进行形态鉴定和多基因系统发育分析,并研究了其生物学特性,同时进行了拮抗链霉菌的筛选鉴定。结果表明,分离到病原菌为瓜类炭疽菌(Colletotrichum orbiculare)。该菌可利用的最佳碳源为淀粉,最佳氮源为硝酸钠、蛋白胨和硝酸钾,不能利用草氨酸。最适宜生长温度为25 ℃,光照和pH值对其生长影响不大。室内平板对峙筛选到2株具有良好拮抗作用的链霉菌F18和F35,F18鉴定为白长链霉菌(Streptomyces albolongus),F35鉴定为抗铅链霉菌(Streptomyces plumbiresistens)。

关键词: 烟草, 炭疽病, 拮抗, 链霉菌

Abstract: A strain of tobacco anthracnose pathogen was isolated from Guizhou Province. In order to clarify the taxonomic status in the genus of Colletotrichum and investigate the suitable Streptomyces biocontrol strain, morphology and polygenic phylogenetic analysis were performed. The biological characteristics were studied and antagonistic Streptomyces were isolated. Results showed that the pathogen was identified as Colletotrichum orbiculare. The best carbon source and nitrogen source for growth was starch and peptone, but it couldn't use oxamic acid. The optimum temperature for growth was 25 ℃. Illumination and pH had little effect on colony diameter. Two strains of Streptomyces F18 and F35 with good antagonistic effects were isolated and preliminarily identified as Streptomyces albolongus and Streptomyces plumbiresistens.

Key words: tobacco, anthracnose, antagonistic, Streptomyces

中图分类号:

S435.72

李继业, 王璟晶, 杨露, 彭丽娟. 烟草炭疽病原研究及拮抗链霉菌鉴定[J]. 浙江农业学报, 2019, 31(8): 1337-1344.

LI Jiye, WANG Jingjing, YANG Lu, PENG Lijuan. Identification of tobacco anthracnose and isolation of antagonistic Streptomyces[J]. , 2019, 31(8): 1337-1344.

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烟草炭疽病原研究及拮抗链霉菌鉴定

Identification of tobacco anthracnose and isolation of antagonistic Streptomyces

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