绵羊GH基因的Pvu Ⅱ 位点的多态性与其生长性状的关联分析

doi:10.3969/j.issn.1004-1524.2019.09.03

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (9): 1416-1422.DOI: 10.3969/j.issn.1004-1524.2019.09.03

绵羊GH基因的Pvu Ⅱ 位点的多态性与其生长性状的关联分析

白俊艳, 曹恒, 王旭, 杨又兵, 樊红灯, 付学言, 时坤鹏, 董智豪, 卢小宁, 李新月, 郝伟光, 李子衡, 郑飞扬

河南科技大学动物科技学院,河南洛阳 471023

收稿日期:2019-03-11 出版日期:2019-09-25 发布日期:2019-10-11
作者简介:白俊艳(1975—),女,内蒙古赤峰人,博士,副教授,主要从事绵羊分子育种研究。E-mail: junyanbai@163.com
基金资助:
国家自然科学基金(31201777)

Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep

BAI Junyan, CAO Heng, WANG Xu, YANG Youbing, FAN Hongdeng, FU Xueyan, SHI Kunpeng, DONG Zhihao, LU Xiaoning, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received:2019-03-11 Online:2019-09-25 Published:2019-10-11

摘要/Abstract

摘要： 为探讨GH基因与绵羊的生长发育性能的关系,采用PCR-RFLP的方法分析了GH基因内含子Ⅱ的PvuⅡ位点在5个绵羊群体中的多态性,并与绵羊体质量和体尺等性状进行了关联分析。结果表明:GH基因内含子Ⅱ的PvuⅡ位点在5个绵羊中检测到两种基因型即AB基因型(264 bp/429 bp/693 bp)和BB基因型(264 bp/429 bp)。大尾寒羊、小尾寒羊、豫西脂尾羊、湖羊、杜泊羊的AB基因型频率分别为0.786、0.750、0.424、0.471、0.459,BB基因型频率分别为0.214、0.250、0.576、0.529、0.541。湖羊的GH基因内含子Ⅱ的PvuⅡ位点显著偏离Hardy-Weinbery平衡状态(P<0.05),小尾寒羊、豫西脂尾羊、杜泊羊的GH基因内含子Ⅱ的的PvuⅡ位点极显著偏离Hardy-Weinbery平衡状态(P<0.01)。关联分析表明,AB基因型绵羊的体质量、体长、胸宽、臀端高、管围、尻高、颈长等指标显著大于BB基因型绵羊(P<0.05)。研究表明,GH基因内含子Ⅱ的PvuⅡ位点对绵羊的生长发育性能有一定影响。

关键词: 绵羊, GH基因, PCR-RFLP, 关联分析

Abstract: To investigate the relationship between GH gene and growth and development performance of sheep, the polymorphism of PvuⅡ locus in intron Ⅱ of GH gene in five sheep populations was analyzed by PCR-RFLP, and the association between the polymorphism of PvuⅡ locus and sheep weight and body size was analyzed. The results showed that two genotypes, AB genotype (264 bp/429 bp/693 bp) and BB genotype (264 bp/429 bp), were detected at PvuⅡ locus of intron Ⅱ of GH gene in five sheep. The AB genotype frequencies of Large-tailed Han sheep, Small-tailed Han sheep, Yuxi fat-tailed sheep, Hu sheep and Dorper sheep were 0.786, 0.750, 0.424, 0.471 and 0.459 respectively, and BB genotype frequencies were 0.214, 0.250, 0.576, 0.529 and 0.541, respectively. The PvuⅡ locus of GH gene intron Ⅱ in Hu sheep deviated significantly from Hardy-Weinbery equilibrium (P<0.05), and the PvuⅡ locus of GH gene intron Ⅱ in Small-tailed han sheep, Yuxi fat-tailed sheep and Dupo sheep deviated significantly from Hardy-Weinbery equilibrium (P<0.01). The correlation analysis showed that the body weight, body length, chest width, buttock height, cannon circumference, rump height and neck length of AB genotype sheep were significantly higher than those of BB genotype sheep (P<0.05). The results showed that the PvuⅡ locus of intron Ⅱ of GH gene had some effects on the growth and development performance of sheep.

Key words: sheep, GH gene, PCR-RFLP, association analysis

中图分类号:

S826

白俊艳, 曹恒, 王旭, 杨又兵, 樊红灯, 付学言, 时坤鹏, 董智豪, 卢小宁, 李新月, 郝伟光, 李子衡, 郑飞扬. 绵羊GH基因的Pvu Ⅱ 位点的多态性与其生长性状的关联分析[J]. 浙江农业学报, 2019, 31(9): 1416-1422.

BAI Junyan, CAO Heng, WANG Xu, YANG Youbing, FAN Hongdeng, FU Xueyan, SHI Kunpeng, DONG Zhihao, LU Xiaoning, LI Xinyue, HAO Weiguang, LI Ziheng, ZHENG Feiyang. Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep[J]. , 2019, 31(9): 1416-1422.

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绵羊GH基因的Pvu Ⅱ 位点的多态性与其生长性状的关联分析

Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep

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