螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

doi:10.3969/j.issn.1004-1524.20231326

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (7): 1511-1518.DOI: 10.3969/j.issn.1004-1524.20231326

螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

李飞¹(), 苏甜甜², 苏康杰², 徐可², 马力², 刘子明²^,^*()

1.唐山海运职业学院海洋工程学院,河北唐山 063200
2.丽水学院生态学院,浙江丽水 323000

收稿日期:2023-11-24 出版日期:2024-07-25 发布日期:2024-08-05
作者简介:李飞(1985—),男,河北唐山人,硕士,讲师,研究方向为水生动物营养。E-mail: lifeirun@163.com
通讯作者: *刘子明,E-mail: lzimin1986@163.com
基金资助:
国家自然科学基金(32002407);丽水市科技计划项目(2023GYX11);丽水市科技计划项目(2021ZDYF09)

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

LI Fei¹(), SU Tiantian², SU Kangjie², XU Ke², MA Li², LIU Ziming²^,^*()

1. College of Ocean Engineering, Tangshan Maritime Institute, Tangshan 063200, Hebei, China
2. College of Ecology, Lishui University, Lishui 323000, Zhejiang, China

Received:2023-11-24 Online:2024-07-25 Published:2024-08-05

摘要/Abstract

摘要：

螺旋藻和雨生红球藻均为药食同源的优质微藻。文章探究饲料同时添加2%或4%的螺旋藻和雨生红球藻对斑马鱼生长性能、抗氧化酶和磷酸酶活性、热休克蛋白表达的影响,为这两种藻类的联合应用奠定基础。实验结果显示,饲料中添加4%螺旋藻+2%红球藻试验组其斑马鱼的终末体重、体重增重率和特定生长率均显著高于对照组,且其淀粉酶和蛋白酶活性也都显著高于对照组。两种藻类的补充对斑马鱼抗氧化酶活性的影响较为复杂,与对照组相比,2%螺旋藻+2%红球藻和4%螺旋藻+2%红球藻这两个试验组斑马鱼的超氧化物歧化酶活性有所下降,4%螺旋藻+2%红球藻和4%螺旋藻+4%红球藻这两个试验组过氧化氢酶活性明显上升。饲料中添加螺旋藻和红球藻能显著提高斑马鱼的磷酸酶活性和热休克蛋白表达,所有藻类添加试验组斑马鱼的磷酸酶活性和热休克蛋白70表达均显著高于对照组,这说明螺旋藻和雨生红球藻的联合投喂有助于斑马鱼免疫相关水平和抗胁迫能力的提高。相比其他试验组,仅4%螺旋藻+2%红球藻试验组斑马鱼的热休克蛋白90表达显著高于对照组。总言之,饲料补充螺旋藻和雨生红球藻有利于提高斑马鱼的生长性能、免疫相关水平和抗应激能力,其中4%螺旋藻+2%红球藻的添加量较为适宜。

关键词: 螺旋藻, 雨生红球藻, 斑马鱼, 生长性能, 抗氧化酶, 磷酸酶, 热休克蛋白

Abstract:

Spirulina platensis and Haematococcus pluvialis are both high-quality microalgae with good nutritional and medicinal value. This study investigated the effects of dietary supplementation of 2% or 4% S. platensis and H. pluvialis on the growth performance, antioxidant enzyme and phosphatase activities, and heat shock protein expression of zebrafish (Danio rerio), which laid the foundation for the joint application of the two algae. The results showed that the final weight, weight gain and specific growth rates of zebrafish in group with 4% S. platensis and 2% H. pluvialis added to feed were significantly higher than those in the control group, and the gains were accompanied by significant increases in amylase and protease activities. The effects of dietary supplementation of two algae on the antioxidant enzyme activities of zebrafish were relatively complex. Compared with the control group, superoxide dismutase activities in two groups (2% S. platensis + 2% H. pluvialis and 4% S. platensis + 2% H. pluvialis) were significantly elevated. The opposite results were observed in catalase activities of zebrafish from two groups (4% S. platensis + 2% H. pluvialis and 4% S. platensis + 4% H. pluvialis). Zebrafish fed a diet supplemented with S. platensis and H. pluvialis had significant elevations in phosphatase activities and the expression of heat shock proteins. Phosphatase activities and heat shock protein 70 expression of zebrafish in all groups with algae supplementation were significantly higher than those in the control group, which illustrated that the combined feeding of S. platensis and H. pluvialis was conducive to improve the immune related levels and stress resistance of zebrafish. Compared with the control group, the significant elevation in the expression of heat shock protein 90 in zebrafish was detected in only one group (4% S. platensis + 2% H. pluvialis) rather than the other three groups. To sum up, dietary supplementation of S. platensis and H. pluvialis improved the growth performance, immune related level and anti-stress ability of zebrafish, and the addition with 4% S. platensis + 2% H. pluvialis was more appropriate.

Key words: Spirulina platensis, Haematococcus pluvialis, zebrafish, growth performance, antioxidant enzyme, phosp hatase, heat shock protein

中图分类号:

S963

李飞, 苏甜甜, 苏康杰, 徐可, 马力, 刘子明. 螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响[J]. 浙江农业学报, 2024, 36(7): 1511-1518.

LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.

图/表 7

参考文献 30

[1]	ROY S S, PAL R. Microalgae in aquaculture: a review with special references to nutritional value and fish dietetics[J]. Proceedings of the Zoological Society, 2015, 68(1): 1-8.
[2]	DE LIMA VALENÇA R, DA SILVA SOBRINHO A G, SILVA L G, et al. Marine algae meal (Schizochytrium sp.) and vitamin E in lamb nutrition: intake, digestibility, nitrogen balance, ingestive behavior, ruminal parameters, performance, and carcass characteristics[J]. Livestock Science, 2022, 258: 104877.
[3]	LUPATINI A L, COLLA L M, CANAN C, et al. Potential application of microalga Spirulina platensis as a protein source[J]. Journal of the Science of Food and Agriculture, 2017, 97(3): 724-732.
[4]	SHALATA H A, BAHATTAB O, ZAYED M M, et al. Synergistic effects of dietary sodium butyrate and Spirulina platensis on growth performance, carcass composition, blood health, and intestinal histomorphology of Nile tilapia (Oreochromis niloticus)[J]. Aquaculture Reports, 2021, 19: 100637.
[5]	RÉGNIER P, BASTIAS J, RODRIGUEZ-RUIZ V, et al. Astaxanthin from Haematococcus pluvialis prevents oxidative stress on human endothelial cells without toxicity[J]. Marine Drugs, 2015, 13(5): 2857-2874.
[6]	POGORZELSKA E, GODZISZEWSKA J, BRODOWSKA M, et al. Antioxidant potential of Haematococcus pluvialis extract rich in astaxanthin on colour and oxidative stability of raw ground pork meat during refrigerated storage[J]. Meat Science, 2018, 135: 54-61.
[7]	LAWRENCE C. The husbandry of zebrafish (Danio rerio): a review[J]. Aquaculture, 2007, 269(1/2/3/4): 1-20.
[8]	HARDY R. Zebrafish as a model fish in nutrition research[J]. Aquaculture Magazine, 2007, 33(4): 81-83.
[9]	GEFFROY B, SIMON O. Effects of a Spirulina platensis-based diet on zebrafish female reproductive performance and larval survival rate[J]. Cybium: International Journal of Ichthyology, 2013, 37(1/2): 31-38.
[10]	SEENIVASAN C, RADHAKRISHNAN S, MURALISANKAR T, et al. Effects of probiotics on survival, growth and digestive enzymes activities in freshwater prawn Macrobrachium rosenbergii(de man 1879)[J]. Proceedings of the Zoological Society, 2016, 69(1): 52-60.
[11]	YUAN Y, JIN M, XIONG J, et al. Effects of dietary dosage forms of copper supplementation on growth, antioxidant capacity, innate immunity enzyme activities and gene expressions for juvenile Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 2019, 84: 1059-1067.
[12]	SAYED A E D H, HAMED M, SOLIMAN H A M. Spirulina platensis alleviated the hemotoxicity, oxidative damage and histopathological alterations of hydroxychloroquine in catfish (Clarias gariepinus)[J]. Frontiers in Physiology, 2021, 12: 683669.
[13]	UMPHAN K M. Growth performance, sex hormone levels and maturation ability of Pla Pho (Pangasius bocourti) fed with Spirulina supplementary pellet and hormone application[J]. International Journal of Agriculture and Biology, 2009, 11(4): 458-462.
[14]	GOGOI S, MANDAL S C, PATEL A B. Effect of dietaryWolffia arrhiza and Spirulina platensison growth performance and pigmentation of Queen loach Botia dario(Hamilton, 1822)[J]. Aquaculture Nutrition, 2018, 24(1): 285-291.
[15]	PALMEGIANO G B, GAI F, DAPRÀ F, et al. Effects of Spirulina and plant oil on the growth and lipid traits of white sturgeon (Acipenser transmontanus) fingerlings[J]. Aquaculture Research, 2008, 39(6): 587-595.
[16]	曹申平, 韩冬, 解绶启, 等. 螺旋藻粉替代饲料中鱼粉对异育银鲫幼鱼生长、饲料利用和蛋白沉积的影响[J]. 水生生物学报, 2016, 40(4): 647-654.
	CAO S P, HAN D, XIE S Q, et al. Effects of dietary fishmeal replacement with Spirulina platensis powder on the growth performance, feed utilization and protein deposition in juvenile gibel carp(Carassis auratus gibelio var. cas)[J]. Acta Hydrobiologica Sinica, 2016, 40(4): 647-654.(in Chinese with English abstract)
[17]	ADEL M, YEGANEH S, DADAR M, et al. Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754)[J]. Fish & Shellfish Immunology, 2016, 56: 436-444.
[18]	YU W, WEN G L, LIN H Z, et al. Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus(Lacepede, 1802)[J]. Fish & Shellfish Immunology, 2018, 74: 649-655.
[19]	ROOHANI A M, ABEDIAN KENARI A, FALLAHI KAPOORCHALI M, et al. Effect of spirulina Spirulina platensisas a complementary ingredient to reduce dietary fish meal on the growth performance, whole-body composition, fatty acid and amino acid profiles, and pigmentation of Caspian brown trout (Salmo trutta caspius) juveniles[J]. Aquaculture Nutrition, 2019, 25(3): 633-645.
[20]	TEIMOURI M, YEGANEH S, MIANJI G R, et al. The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss)[J]. Fish Physiology and Biochemistry, 2019, 45(3): 977-986.
[21]	MAHMOUD M M A, EL-LAMIE M M M, KILANY O E, et al. Spirulina(Arthrospira platensis) supplementation improves growth performance, feed utilization, immune response, and relieves oxidative stress in Nile tilapia (Oreochromis niloticus) challenged with Pseudomonas fluorescens[J]. Fish & Shellfish Immunology, 2018, 72: 291-300.
[22]	LI H, LIU J G. Supplementation of Moina macrocopa with defatted Haematococcus pluvialis meal improved its growth performance and nutritional quality[J]. Aquaculture, 2021, 534: 736164.
[23]	CAI M L, HUI W J, DENG X, et al. Dietary Haematococcus pluvialis promotes growth of red swamp crayfish Procambarus clarkii(Girard, 1852) via positive regulation of the gut microbial co-occurrence network[J]. Aquaculture, 2022, 551: 737900.
[24]	梁倩蓉, 郑天伦, 陈小明, 等. 饲料中添加蝇蛆蛋白对中华鳖肝和血清免疫代谢应答的影响[J]. 浙江农业学报, 2022, 34(10): 2172-2181.
	LIANG Q R, ZHENG T L, CHEN X M, et al. Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2172-2181.(in Chinese with English abstract)
[25]	武萌萌, 刘士伟, 李博生. 螺旋藻小分子多肽制备工艺的研究[J]. 浙江农业学报, 2010, 22(6): 802-807.
	WU M M, LIU S W, LI B S. Preparation and purification of low-molecular-weight peptides from Spirulina protein[J]. Acta Agriculturae Zhejiangensis, 2010, 22(6): 802-807.(in Chinese with English abstract)
[26]	JIANG M, ZHAO H H, ZAI S W, et al. A defatted microalgae meal (Haematococcus pluvialis) as a partial protein source to replace fishmeal for feeding juvenile yellow perch Perca flavescens[J]. Journal of Applied Phycology, 2019, 31(2): 1197-1205.
[27]	JOSHUA W J, ZULPERI Z. Effects of Spirulina platensis and Chlorella vulgaris on the immune system and reproduction of fish[J]. Pertanika Journal of Tropical Agricultural Science, 2020, 43(4): 429-444.
[28]	YU W J, ZHANG M J, WANG B J, et al. Dietary Haematococcus pluvialis powder supplementation affect carotenoid content, astaxanthin isomer, antioxidant capacity and immune-related gene expression in Pacific white shrimp, Litopenaeus vannamei[J]. Aquaculture Research, 2021, 52(6): 2403-2414.
[29]	PHAM T H, CHENG T C, WANG P C, et al. Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer)[J]. Fish & Shellfish Immunology, 2021, 111: 179-188.
[30]	LEE D C, CHOI Y J, KIM J H. Toxic effects of waterborne cadmium exposure on hematological parameters, oxidative stress, neurotoxicity, and heat shock protein 70 in juvenile olive flounder, Paralichthys olivaceus[J]. Fish & Shellfish Immunology, 2022, 122: 476-483.

项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

处理 Treatment	初始体重 Initial weight/g	终末体重 Terminal weight/g	体重增重率 Weight gain rate/%	特定生长率 Specific growth rate/(%·d^-1)	成活率 Survival rate/%
CK	0.025 5±0.001 2 a	0.061 1±0.003 1 a	139.5±10.2 a	1.455±0.072 a	96.27±2.89 a
SP2-HP2	0.025 1±0.000 8 a	0.072 1±0.010 5 ab	186.8±36.9 ab	1.747±0.215 bc	98.33±2.89 a
SP2-HP4	0.026 1±0.001 0 a	0.066 8±0.006 7 ab	156.0±17.3 a	1.564±0.113 ab	98.33±2.89 a
SP4-HP2	0.026 0±0.000 4 a	0.079 5±0.007 2 b	205.7±22.4 b	1.859±0.121 c	100.00±0.00 a
SP4-HP4	0.025 4±0.000 5 a	0.071 0±0.009 9 ab	179.8±37.2 ab	1.705±0.214 abc	98.33±2.89 a

螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

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