螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

doi:10.3969/j.issn.1004-1524.20231326

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (7): 1511-1518.DOI: 10.3969/j.issn.1004-1524.20231326

螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

李飞¹(), 苏甜甜², 苏康杰², 徐可², 马力², 刘子明²^,^*()

1.唐山海运职业学院海洋工程学院,河北唐山 063200
2.丽水学院生态学院,浙江丽水 323000

收稿日期:2023-11-24 出版日期:2024-07-25 发布日期:2024-08-05
作者简介:李飞(1985—),男,河北唐山人,硕士,讲师,研究方向为水生动物营养。E-mail: lifeirun@163.com
通讯作者: *刘子明,E-mail: lzimin1986@163.com
基金资助:
国家自然科学基金(32002407);丽水市科技计划项目(2023GYX11);丽水市科技计划项目(2021ZDYF09)

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

LI Fei¹(), SU Tiantian², SU Kangjie², XU Ke², MA Li², LIU Ziming²^,^*()

1. College of Ocean Engineering, Tangshan Maritime Institute, Tangshan 063200, Hebei, China
2. College of Ecology, Lishui University, Lishui 323000, Zhejiang, China

Received:2023-11-24 Online:2024-07-25 Published:2024-08-05

摘要/Abstract

摘要：

螺旋藻和雨生红球藻均为药食同源的优质微藻。文章探究饲料同时添加2%或4%的螺旋藻和雨生红球藻对斑马鱼生长性能、抗氧化酶和磷酸酶活性、热休克蛋白表达的影响,为这两种藻类的联合应用奠定基础。实验结果显示,饲料中添加4%螺旋藻+2%红球藻试验组其斑马鱼的终末体重、体重增重率和特定生长率均显著高于对照组,且其淀粉酶和蛋白酶活性也都显著高于对照组。两种藻类的补充对斑马鱼抗氧化酶活性的影响较为复杂,与对照组相比,2%螺旋藻+2%红球藻和4%螺旋藻+2%红球藻这两个试验组斑马鱼的超氧化物歧化酶活性有所下降,4%螺旋藻+2%红球藻和4%螺旋藻+4%红球藻这两个试验组过氧化氢酶活性明显上升。饲料中添加螺旋藻和红球藻能显著提高斑马鱼的磷酸酶活性和热休克蛋白表达,所有藻类添加试验组斑马鱼的磷酸酶活性和热休克蛋白70表达均显著高于对照组,这说明螺旋藻和雨生红球藻的联合投喂有助于斑马鱼免疫相关水平和抗胁迫能力的提高。相比其他试验组,仅4%螺旋藻+2%红球藻试验组斑马鱼的热休克蛋白90表达显著高于对照组。总言之,饲料补充螺旋藻和雨生红球藻有利于提高斑马鱼的生长性能、免疫相关水平和抗应激能力,其中4%螺旋藻+2%红球藻的添加量较为适宜。

关键词: 螺旋藻, 雨生红球藻, 斑马鱼, 生长性能, 抗氧化酶, 磷酸酶, 热休克蛋白

Abstract:

Spirulina platensis and Haematococcus pluvialis are both high-quality microalgae with good nutritional and medicinal value. This study investigated the effects of dietary supplementation of 2% or 4% S. platensis and H. pluvialis on the growth performance, antioxidant enzyme and phosphatase activities, and heat shock protein expression of zebrafish (Danio rerio), which laid the foundation for the joint application of the two algae. The results showed that the final weight, weight gain and specific growth rates of zebrafish in group with 4% S. platensis and 2% H. pluvialis added to feed were significantly higher than those in the control group, and the gains were accompanied by significant increases in amylase and protease activities. The effects of dietary supplementation of two algae on the antioxidant enzyme activities of zebrafish were relatively complex. Compared with the control group, superoxide dismutase activities in two groups (2% S. platensis + 2% H. pluvialis and 4% S. platensis + 2% H. pluvialis) were significantly elevated. The opposite results were observed in catalase activities of zebrafish from two groups (4% S. platensis + 2% H. pluvialis and 4% S. platensis + 4% H. pluvialis). Zebrafish fed a diet supplemented with S. platensis and H. pluvialis had significant elevations in phosphatase activities and the expression of heat shock proteins. Phosphatase activities and heat shock protein 70 expression of zebrafish in all groups with algae supplementation were significantly higher than those in the control group, which illustrated that the combined feeding of S. platensis and H. pluvialis was conducive to improve the immune related levels and stress resistance of zebrafish. Compared with the control group, the significant elevation in the expression of heat shock protein 90 in zebrafish was detected in only one group (4% S. platensis + 2% H. pluvialis) rather than the other three groups. To sum up, dietary supplementation of S. platensis and H. pluvialis improved the growth performance, immune related level and anti-stress ability of zebrafish, and the addition with 4% S. platensis + 2% H. pluvialis was more appropriate.

Key words: Spirulina platensis, Haematococcus pluvialis, zebrafish, growth performance, antioxidant enzyme, phosp hatase, heat shock protein

中图分类号:

S963

李飞, 苏甜甜, 苏康杰, 徐可, 马力, 刘子明. 螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响[J]. 浙江农业学报, 2024, 36(7): 1511-1518.

LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.

图/表 7

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项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

项目Item		对照组CK	SP2-HP2	SP2-HP4	SP4-HP2	SP4-HP4
成分	鱼粉Fish meal	50	46	44	44	42
Component/(g·kg^-1)	螺旋藻Spirulina	0	2	2	4	4
	雨生红球藻Haematococcus pluvialis	0	2	4	2	4
	小麦粉Wheat flour	16	16	16	16	16
	豆粉Soybean flour	16	16	16	16	16
	玉米淀粉Corn starch	10	10	10	10	10
	鱼油Fish oil	3	3	3	3	3
	豆油Soybean oil	3	3	3	3	3
	多维预混物Multivitamin premix ¹	1	1	1	1	1
	多矿预混物Multimineral premix ²	1	1	1	1	1
生化组成	粗蛋白Crude protein	45.54	45.50	45.43	45.31	45.09
Biochemical composition/%	粗脂肪Crude fat	9.08	9.15	9.21	9.24	9.28
	灰分Ash	7.65	7.67	7.73	7.74	7.69
	水分Water	8.64	8.58	8.67	8.55	8.60

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

基因 Gene	引物序列 Primer sequence(5'-3')	GenBank编录号 GenBank accession No.	产物长度 Product length/bp
HSP70	F: CATGAAGAGCAGTGTGGAAGAC	AF210640	182
	R: TGGAGATGACTGGATTGCAG
HSP90	F: CCCATTGTGGAGACCCTCAGA	AF068773	179
	R: TCATCGATGCCAAGTCCGAG
β-actin	F: CATGGCTTCTGCTCTGTATG	AF026063	143
	R: GCAAAGTGGTAAACGCTTCT

处理 Treatment	初始体重 Initial weight/g	终末体重 Terminal weight/g	体重增重率 Weight gain rate/%	特定生长率 Specific growth rate/(%·d^-1)	成活率 Survival rate/%
CK	0.025 5±0.001 2 a	0.061 1±0.003 1 a	139.5±10.2 a	1.455±0.072 a	96.27±2.89 a
SP2-HP2	0.025 1±0.000 8 a	0.072 1±0.010 5 ab	186.8±36.9 ab	1.747±0.215 bc	98.33±2.89 a
SP2-HP4	0.026 1±0.001 0 a	0.066 8±0.006 7 ab	156.0±17.3 a	1.564±0.113 ab	98.33±2.89 a
SP4-HP2	0.026 0±0.000 4 a	0.079 5±0.007 2 b	205.7±22.4 b	1.859±0.121 c	100.00±0.00 a
SP4-HP4	0.025 4±0.000 5 a	0.071 0±0.009 9 ab	179.8±37.2 ab	1.705±0.214 abc	98.33±2.89 a

螺旋藻和红球藻对斑马鱼生长性能、抗氧化酶、磷酸酶和热休克蛋白的影响

Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio)

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