甜樱桃PP2C家族全基因组鉴定与表达分析

doi:10.3969/j.issn.1004-1524.20240162

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (10): 2204-2218.DOI: 10.3969/j.issn.1004-1524.20240162

甜樱桃PP2C家族全基因组鉴定与表达分析

刘博华¹(), 张庆霞², 祁亮¹, 吴玉霞¹, 王延秀¹^,^*()

1.甘肃农业大学园艺学院,甘肃兰州 730070
2.陇东学院农林科技学院,甘肃庆阳 745000

收稿日期:2024-02-22 出版日期:2024-10-25 发布日期:2024-10-30
作者简介:刘博华(2001—),男,甘肃天水人,硕士,主要从事果树种质资源与遗传育种研究。E-mail:1830521903@qq.com
通讯作者: ^*王延秀,E-mail:wangxy@gsau.edu.cn
基金资助:
甘肃省科技厅技术创新引导计划(21CX6NM108);甘肃农业大学自列项目(701-0722105)

Genomic-wide identification and expression analysis of the PP2C gene family in sweet cherry

LIU Bohua¹(), ZHANG Qingxia², QI Liang¹, WU Yuxia¹, WANG Yanxiu¹^,^*()

1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China
2. College of Agriculture and Forestry Science and Technology, Longdong University,Qingyang 745000, Gansu,China

Received:2024-02-22 Online:2024-10-25 Published:2024-10-30

摘要/Abstract

摘要：

蛋白磷酸酶(protein phosphatase 2C, PP2C)作为ABA信号通路关键调控因子,在植物生长、激素传导和逆境响应中起重要作用。鉴定和分析甜樱桃PP2C基因家族对不同非生物胁迫的响应,以探究甜樱桃中该家族成员的抗逆功能。于蔷薇科基因组数据库 (https://www. rosaceae. org/)中下载甜樱桃全基因组文件,利用生物信息学研究其蛋白理化性质、亲缘关系、基因结构、染色体定位和共线性、启动子顺式元件等。以樱桃吉塞拉7号试管苗为材料,通过实时荧光定量PCR(qRT-PCR)进行表达模式分析。甜樱桃PP2C家族中共鉴定出76个成员,其蛋白质序列长度介于125~1 580个氨基酸之间,理论等电点(pI)在4.57~9.40,72%成员为酸性蛋白。染色体定位表明它们不均匀地分布在所有染色体上,包括14对共线性基因,可分为12个亚家族。亚细胞定位预测显示该家族成员主要分布于细胞核中。顺式作用元件分析表明,76个PaPP2C基因上游2 000 bp启动子区域均含有响应激素、光和环境胁迫的元件。挑选10个基因进行qRT-PCR分析发现,PaPP2C23、PaPP2C47、PaPP2C48、PaPP2C49这4个基因在干旱(PEG)、氯化钠(NaCl)和脱落酸(ABA)胁迫下均显著上调,推测以上基因可以通过依赖脱落酸调控干旱和氯化钠胁迫;在低温(LT)条件下,PaPP2C71、PaPP2C5、PaPP2C49、PaPP2C23、PaPP2C59、PaPP2C69、PaPP2C60基因显著下调,表明它们可能在低温胁迫中起负调控作用。甜樱桃PP2C家族基因成员可在干旱、氯化钠、低温以及脱落酸等非生物逆境中发挥作用。

关键词: 甜樱桃2C类蛋白磷酸酶, 干旱, 低温, 顺式作用元件

Abstract:

Protein phosphatase (protein phosphatase 2C, PP2C), as a key regulator of ABA signaling pathway, plays an important role in plant growth, hormone transduction and stress response. To identify and analyze the response of sweet cherry PP2C gene family to different abiotic stresses, the stress resistance function of this family members were explored in sweet cherry, in order to provide a theoretical basis for improving their stress resistance by genetic engineering technology. The whole genome file was downloaded from Rosaceae genome database (https://www. rosaceae.org/). Bioinformatics was used to study the physicochemical properties, genetic relationships, gene structure, chromosome localization and collinearity, promoter cis-elements, etc. Cherry Gisela No. 7 test-tube seedlings were used as materials,real-time fluorescence quantification PCR (qRT-PCR) was used to analyze the expression patterns. A total of 76 members of the sweet Cherry PP2C family were identified, whose protein sequence length ranged from 125 to 1 580 amino acids, theoretical isoelectric point (pI) ranged from 4.57 to 9.40, and 72% of the members were acidic proteins. Chromosomal localization showed that they were unevenly distributed on 8 chromosomes of sweet cherry, including 14 collinear gene pairs, which could be divided into 12 subfamilies. Subcellular localization prediction showed that the family members were mainly distributed in the nucleus. Additionally, Analysis of cis-acting elements displayed that the upstream 2 000 bp promoter region of 76 PaPP2C genes contained multiple elements, such as elements of hormone, light and environmental stress. The results of qRT-PCR analysis of 10 genes showed that PaPP2C23, PaPP2C47, PaPP2C48 and PaPP2C49 genes were significantly up-regulated under drought (PEG), salt (NaCl) and abscisic acid (ABA) stresses, which was speculated that the above genes could regulate drought and salt stress by relying on abscisic acid. Additionally, PaPP2C71, PaPP2C5, PaPP2C49, PaPP2C23, PaPP2C59,PaPP2C60 and PaPP2C74 were significantly down-regulated, indicating that they might play negative regulatory role in low temperature stress. Sweet cherry PP2C family members can play important role in biotic stresses such as drought, salt, low temperature, and abscisic acid.

Key words: sweet cherry 2C protein phosphatase, drought, microtherm, cis-acting element

中图分类号:

S662.5

刘博华, 张庆霞, 祁亮, 吴玉霞, 王延秀. 甜樱桃PP2C家族全基因组鉴定与表达分析[J]. 浙江农业学报, 2024, 36(10): 2204-2218.

LIU Bohua, ZHANG Qingxia, QI Liang, WU Yuxia, WANG Yanxiu. Genomic-wide identification and expression analysis of the PP2C gene family in sweet cherry[J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2204-2218.

图/表 12

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基因 Gene	正向引物 Forward primer(5’→3’)	反向引物 Reverse primer(5’→3’)
PaPP2C5	TATGTCAGCGGACCCTATTTGTGG	TTTCAGTTCGTGCCTAACCTCCTC
PaPP2C23	CACCGTGTTGGAAACCTTCTGTTG	AACCATCAAGCCGACCACTCAC
PaPP2C47	TGGATCACAGCACCAGTGTAGAAG	CACGGTCATTCATCACAGCACAAG
PaPP2C48	GCTTCTCGCTTCATCACCAATCG	CCAAGTCCAAGAACTCCTCCTCAG
PaPP2C49	AGCAGCCAAGAAGAGAGAAGTGAG	CACCACAACACTGATGTCGTCATG
PaPP2C71	GGCTTTGGCACATGGACTTGAAG	ACATAGGTCGCAGAGGGAGAGG
PaPP2C39	GGCTTTGGCACATGGACTTGAAG	ACATAGGTCGCAGAGGGAGAGG
PaPP2C74	AAGTTTCGTCTTCGGGACCCTATC	TCCACTGCTTCCTGATTGCTGAG
PaPP2C59	CCAGTGAGGAGATATGCCCGTATG	TAGGAGTGCTTGTCAAGGCTTCTG
PaPP2C60	AGGTTCACGTCAGAGCAACAGTC	CACACCAACAAGGCAACAAGACC
PaRPL	CTTGCATCCCTCAGCACCTT	TCCTGTGGACAATGGATGGA

基因 Gene	正向引物 Forward primer(5’→3’)	反向引物 Reverse primer(5’→3’)
PaPP2C5	TATGTCAGCGGACCCTATTTGTGG	TTTCAGTTCGTGCCTAACCTCCTC
PaPP2C23	CACCGTGTTGGAAACCTTCTGTTG	AACCATCAAGCCGACCACTCAC
PaPP2C47	TGGATCACAGCACCAGTGTAGAAG	CACGGTCATTCATCACAGCACAAG
PaPP2C48	GCTTCTCGCTTCATCACCAATCG	CCAAGTCCAAGAACTCCTCCTCAG
PaPP2C49	AGCAGCCAAGAAGAGAGAAGTGAG	CACCACAACACTGATGTCGTCATG
PaPP2C71	GGCTTTGGCACATGGACTTGAAG	ACATAGGTCGCAGAGGGAGAGG
PaPP2C39	GGCTTTGGCACATGGACTTGAAG	ACATAGGTCGCAGAGGGAGAGG
PaPP2C74	AAGTTTCGTCTTCGGGACCCTATC	TCCACTGCTTCCTGATTGCTGAG
PaPP2C59	CCAGTGAGGAGATATGCCCGTATG	TAGGAGTGCTTGTCAAGGCTTCTG
PaPP2C60	AGGTTCACGTCAGAGCAACAGTC	CACACCAACAAGGCAACAAGACC
PaRPL	CTTGCATCCCTCAGCACCTT	TCCTGTGGACAATGGATGGA

基因名 Gene name	基因登录号 Protein length	蛋白质长度 Protein length	分子量 Molecular weight/u	等电点 Isoelectric point	脂肪系数(AI) Fat coefficient	亲水性 Hydro- philic	亚细胞定位 Subcellular localization
PaPP2C1	FUN_000049-T1	299	32 772.11	8.78	82.47	-0.393	细胞质 Cyto
PaPP2C2	FUN_000449-T1	274	30 756.99	9.40	71.20	-0.514	细胞核 Nucl
PaPP2C3	FUN_001229-T1	292	31 498.96	5.04	90.90	-0.178	细胞质 Cyto
PaPP2C4	FUN_001229-T2	429	46 011.36	7.97	91.70	-0.275	叶绿体 Chlo
PaPP2C5	FUN_001284-T1	381	43 076.32	7.24	88.51	-0.229	线粒体 Mito
PaPP2C6	FUN_001438-T1	503	55 656.18	4.83	85.85	-0.205	细胞核 Nucl
PaPP2C7	FUN_001554-T1	390	42 928.29	5.03	83.54	-0.245	叶绿体 Chlo
PaPP2C8	FUN_001678-T1	360	39 919.86	6.57	75.57	-0.354	细胞质 Cyto
PaPP2C9	FUN_001678-T2	458	50 538.34	7.20	75.74	-0.355	细胞质 Cyto
PaPP2C10	FUN_001751-T1	473	52 530.67	5.01	92.73	-0.244	细胞核 Nucl
PaPP2C11	FUN_002149-T1	324	34 682.53	4.58	82.77	-0.165	细胞质 Cyto
PaPP2C12	FUN_003811-T1	555	59 538.47	4.95	78.14	-0.558	叶绿体 Chlo
PaPP2C13	FUN_004617-T1	391	43 659.74	8.78	83.22	-0.216	叶绿体 Chlo
PaPP2C14	FUN_005544-T1	318	35 323.97	5.61	71.45	-0.426	细胞核 Nucl
PaPP2C15	FUN_005584-T1	424	46 316.56	4.97	78.84	-0.345	细胞核 Nucl
PaPP2C16	FUN_005691-T1	376	41 771.88	8.23	90.53	-0.191	细胞核 Nucl
PaPP2C17	FUN_005753-T1	370	41 228.32	9.03	88.85	-0.244	细胞质 Cyto
PaPP2C18	FUN_006003-T1	1 080	119 451.84	5.00	80.93	-0.296	线粒体 Plas
PaPP2C19	FUN_006358-T1	525	57 481.63	4.83	75.17	-0.323	细胞核 Nucl
PaPP2C20	FUN_006957-T1	364	39 697.60	5.49	78.12	-0.421	叶绿体 Chlo
PaPP2C21	FUN_010994-T1	348	38 675.74	5.98	75.70	-0.327	叶绿体 Chlo
PaPP2C22	FUN_011249-T1	192	21 758.86	9.04	96.93	-0.211	叶绿体 Chlo
PaPP2C23	FUN_012008-T1	372	41 673.43	8.49	96.18	-0.211	叶绿体 Chlo
PaPP2C24	FUN_012215-T1	404	43 999.09	6.13	78.24	-0.380	细胞核 Nucl
PaPP2C25	FUN_012345-T1	910	100 670.95	5.86	79.84	-0.243	细胞核 Nucl
PaPP2C26	FUN_012944-T1	375	41 263.70	5.46	76.52	-0.355	细胞核 Nucl
PaPP2C27	FUN_012979-T1	423	46 284.55	5.02	81.87	-0.419	细胞核 Nucl
PaPP2C28	FUN_013000-T1	401	43 713.38	6.72	87.96	-0.167	细胞核 Nucl
PaPP2C29	FUN_013346-T1	387	43 075.85	5.01	70.26	-0.553	细胞核 Nucl
PaPP2C30	FUN_013425-T1	551	60 209.03	4.57	83.75	-0.240	叶绿体 Chlo
PaPP2C31	FUN_013425-T2	349	38 280.17	5.18	84.06	-0.164	细胞核 Nucl
PaPP2C32	FUN_013425-T3	360	40 378.75	4.97	89.14	-0.185	叶绿体 Chlo
PaPP2C33	FUN_013855-T1	572	63 511.52	5.49	94.50	-0.091	叶绿体 Chlo
PaPP2C34	FUN_014622-T1	299	32 772.11	8.78	75.30	-0.430	细胞质 Cyto
PaPP2C35	FUN_016958-T1	282	31 165.29	5.86	71.40	-0.459	细胞质 Cyto
PaPP2C36	FUN_033875-T1	300	32 800.15	5.60	87.09	-0.347	叶绿体 Chlo
PaPP2C37	FUN_023642-T1	735	81 531.14	5.40	101.96	-0.009	细胞核 Nucl
PaPP2C38	FUN_024151-T1	125	13 596.59	5.87	92.80	0.018	叶绿体 Chlo
PaPP2C39	FUN_024431-T1	777	85 920.90	5.46	74.14	-0.554	细胞质 Cyto
PaPP2C40	FUN_025296-T1	387	42 494.09	5.79	76.08	-0.319	叶绿体 Chlo
PaPP2C41	FUN_026503-T1	378	42 119.06	5.93	92.78	-0.087	细胞质 Cyto
PaPP2C42	FUN_026534-T1	471	52 161.40	5.70	90.64	-0.175	叶绿体 Chlo
PaPP2C43	FUN_018271-T1	396	43 302.83	6.54	75.70	-0.327	细胞质 Cyto
PaPP2C44	FUN_018698-T1	214	23 358.02	5.97	82.84	-0.374	叶绿体 Chlo
PaPP2C45	FUN_018890-T1	426	46 154.79	5.71	84.37	-0.212	细胞核 Nucl
PaPP2C46	FUN_019058-T1	510	56 082.40	6.42	74.14	-0.384	细胞核 Nucl
PaPP2C47	FUN_019150-T1	1 580	174 898.72	5.20	76.91	-0.492	线粒体 Plas
PaPP2C48	FUN_021542-T1	590	65 183.93	8.61	94.07	-0.152	线粒体 Plas
PaPP2C49	FUN_021766-T1	381	42 358.23	8.52	93.78	-0.269	叶绿体 Chlo
PaPP2C50	FUN_021880-T1	697	77 303.59	5.63	73.73	-0.559	细胞核 Nucl
PaPP2C51	FUN_022286-T1	386	41 852.44	5.58	74.14	-0.557	细胞核 Nucl
PaPP2C52	FUN_022643-T1	293	31 769.60	4.88	93.82	-0.041	叶绿体 Chlo
PaPP2C53	FUN_022651-T1	373	41 215.74	6.32	79.73	-0.279	细胞核 Nucl
PaPP2C54	FUN_023101-T1	473	52 530.67	5.01	73.84	-0.517	细胞核 Nucl
PaPP2C55	FUN_037105-T1	371	39 770.99	5.95	95.39	-0.055	叶绿体 Chlo
PaPP2C56	FUN_037105-T2	518	57 007.52	5.26	93.44	-0.065	叶绿体 Chlo
PaPP2C57	FUN_037109-T1	397	44 043.49	4.81	95.53	-0.063	细胞核 Nucl
PaPP2C58	FUN_037397-T1	352	38 049.98	6.37	81.76	-0.382	叶绿体 Chlo
PaPP2C59	FUN_037631-T1	324	43 682.53	4.58	90.31	-0.263	细胞质 Cyto
PaPP2C60	FUN_038803-T1	397	44 146.12	8.44	88.52	-0.296	细胞核 Nucl
PaPP2C61	FUN_038803-T2	283	30 877.72	7.20	88.47	-0.300	叶绿体 Chlo
PaPP2C62	FUN_038943-T1	382	42 478.4	7.04	82.53	-0.277	叶绿体 Chlo
PaPP2C63	FUN_039200-T1	282	30 814.64	8.67	78.16	-0.320	叶绿体 Chlo
PaPP2C64	FUN_039354-T1	371	40 351.93	6.27	86.21	-0.281	叶绿体 Chlo
PaPP2C65	FUN_039354-T2	347	37 804.29	5.16	81.81	-0.170	叶绿体 Chlo
PaPP2C66	FUN_039387-T1	384	41 605.13	5.31	90.08	-0.147	叶绿体 Chlo
PaPP2C67	FUN_040333-T1	366	40 043.93	5.06	85.89	-0.303	叶绿体 Chlo
PaPP2C68	FUN_027600-T1	300	32 800.15	5.60	76.85	-0.236	叶绿体 Chlo
PaPP2C69	FUN_027658-T1	390	43 288.57	7.76	76.85	-0.236	叶绿体 Chlo
PaPP2C70	FUN_028496-T1	360	40 378.75	4.97	81.63	-0.418	叶绿体 Chlo
PaPP2C71	FUN_029922-T1	381	42 358.23	8.52	87.98	-0.302	叶绿体 Chlo
PaPP2C72	FUN_029922-T2	1 064	119 890.36	5.72	90.83	-0.130	叶绿体 Chlo
PaPP2C73	FUN_029973-T1	326	36 003.06	7.15	87.98	-0.302	细胞质 Cyto
PaPP2C74	FUN_030294-T1	632	69 772.38	8.68	88.87	-0.324	线粒体 Plas
PaPP2C75	FUN_030459-T1	400	44 247.33	5.41	79.61	-0.238	叶绿体 Chlo
PaPP2C76	FUN_031081-T1	628	69 390.53	5.73	83.15	-0.318	叶绿体 Chlo

基因名 Gene name	基因登录号 Protein length	蛋白质长度 Protein length	分子量 Molecular weight/u	等电点 Isoelectric point	脂肪系数(AI) Fat coefficient	亲水性 Hydro- philic	亚细胞定位 Subcellular localization
PaPP2C1	FUN_000049-T1	299	32 772.11	8.78	82.47	-0.393	细胞质 Cyto
PaPP2C2	FUN_000449-T1	274	30 756.99	9.40	71.20	-0.514	细胞核 Nucl
PaPP2C3	FUN_001229-T1	292	31 498.96	5.04	90.90	-0.178	细胞质 Cyto
PaPP2C4	FUN_001229-T2	429	46 011.36	7.97	91.70	-0.275	叶绿体 Chlo
PaPP2C5	FUN_001284-T1	381	43 076.32	7.24	88.51	-0.229	线粒体 Mito
PaPP2C6	FUN_001438-T1	503	55 656.18	4.83	85.85	-0.205	细胞核 Nucl
PaPP2C7	FUN_001554-T1	390	42 928.29	5.03	83.54	-0.245	叶绿体 Chlo
PaPP2C8	FUN_001678-T1	360	39 919.86	6.57	75.57	-0.354	细胞质 Cyto
PaPP2C9	FUN_001678-T2	458	50 538.34	7.20	75.74	-0.355	细胞质 Cyto
PaPP2C10	FUN_001751-T1	473	52 530.67	5.01	92.73	-0.244	细胞核 Nucl
PaPP2C11	FUN_002149-T1	324	34 682.53	4.58	82.77	-0.165	细胞质 Cyto
PaPP2C12	FUN_003811-T1	555	59 538.47	4.95	78.14	-0.558	叶绿体 Chlo
PaPP2C13	FUN_004617-T1	391	43 659.74	8.78	83.22	-0.216	叶绿体 Chlo
PaPP2C14	FUN_005544-T1	318	35 323.97	5.61	71.45	-0.426	细胞核 Nucl
PaPP2C15	FUN_005584-T1	424	46 316.56	4.97	78.84	-0.345	细胞核 Nucl
PaPP2C16	FUN_005691-T1	376	41 771.88	8.23	90.53	-0.191	细胞核 Nucl
PaPP2C17	FUN_005753-T1	370	41 228.32	9.03	88.85	-0.244	细胞质 Cyto
PaPP2C18	FUN_006003-T1	1 080	119 451.84	5.00	80.93	-0.296	线粒体 Plas
PaPP2C19	FUN_006358-T1	525	57 481.63	4.83	75.17	-0.323	细胞核 Nucl
PaPP2C20	FUN_006957-T1	364	39 697.60	5.49	78.12	-0.421	叶绿体 Chlo
PaPP2C21	FUN_010994-T1	348	38 675.74	5.98	75.70	-0.327	叶绿体 Chlo
PaPP2C22	FUN_011249-T1	192	21 758.86	9.04	96.93	-0.211	叶绿体 Chlo
PaPP2C23	FUN_012008-T1	372	41 673.43	8.49	96.18	-0.211	叶绿体 Chlo
PaPP2C24	FUN_012215-T1	404	43 999.09	6.13	78.24	-0.380	细胞核 Nucl
PaPP2C25	FUN_012345-T1	910	100 670.95	5.86	79.84	-0.243	细胞核 Nucl
PaPP2C26	FUN_012944-T1	375	41 263.70	5.46	76.52	-0.355	细胞核 Nucl
PaPP2C27	FUN_012979-T1	423	46 284.55	5.02	81.87	-0.419	细胞核 Nucl
PaPP2C28	FUN_013000-T1	401	43 713.38	6.72	87.96	-0.167	细胞核 Nucl
PaPP2C29	FUN_013346-T1	387	43 075.85	5.01	70.26	-0.553	细胞核 Nucl
PaPP2C30	FUN_013425-T1	551	60 209.03	4.57	83.75	-0.240	叶绿体 Chlo
PaPP2C31	FUN_013425-T2	349	38 280.17	5.18	84.06	-0.164	细胞核 Nucl
PaPP2C32	FUN_013425-T3	360	40 378.75	4.97	89.14	-0.185	叶绿体 Chlo
PaPP2C33	FUN_013855-T1	572	63 511.52	5.49	94.50	-0.091	叶绿体 Chlo
PaPP2C34	FUN_014622-T1	299	32 772.11	8.78	75.30	-0.430	细胞质 Cyto
PaPP2C35	FUN_016958-T1	282	31 165.29	5.86	71.40	-0.459	细胞质 Cyto
PaPP2C36	FUN_033875-T1	300	32 800.15	5.60	87.09	-0.347	叶绿体 Chlo
PaPP2C37	FUN_023642-T1	735	81 531.14	5.40	101.96	-0.009	细胞核 Nucl
PaPP2C38	FUN_024151-T1	125	13 596.59	5.87	92.80	0.018	叶绿体 Chlo
PaPP2C39	FUN_024431-T1	777	85 920.90	5.46	74.14	-0.554	细胞质 Cyto
PaPP2C40	FUN_025296-T1	387	42 494.09	5.79	76.08	-0.319	叶绿体 Chlo
PaPP2C41	FUN_026503-T1	378	42 119.06	5.93	92.78	-0.087	细胞质 Cyto
PaPP2C42	FUN_026534-T1	471	52 161.40	5.70	90.64	-0.175	叶绿体 Chlo
PaPP2C43	FUN_018271-T1	396	43 302.83	6.54	75.70	-0.327	细胞质 Cyto
PaPP2C44	FUN_018698-T1	214	23 358.02	5.97	82.84	-0.374	叶绿体 Chlo
PaPP2C45	FUN_018890-T1	426	46 154.79	5.71	84.37	-0.212	细胞核 Nucl
PaPP2C46	FUN_019058-T1	510	56 082.40	6.42	74.14	-0.384	细胞核 Nucl
PaPP2C47	FUN_019150-T1	1 580	174 898.72	5.20	76.91	-0.492	线粒体 Plas
PaPP2C48	FUN_021542-T1	590	65 183.93	8.61	94.07	-0.152	线粒体 Plas
PaPP2C49	FUN_021766-T1	381	42 358.23	8.52	93.78	-0.269	叶绿体 Chlo
PaPP2C50	FUN_021880-T1	697	77 303.59	5.63	73.73	-0.559	细胞核 Nucl
PaPP2C51	FUN_022286-T1	386	41 852.44	5.58	74.14	-0.557	细胞核 Nucl
PaPP2C52	FUN_022643-T1	293	31 769.60	4.88	93.82	-0.041	叶绿体 Chlo
PaPP2C53	FUN_022651-T1	373	41 215.74	6.32	79.73	-0.279	细胞核 Nucl
PaPP2C54	FUN_023101-T1	473	52 530.67	5.01	73.84	-0.517	细胞核 Nucl
PaPP2C55	FUN_037105-T1	371	39 770.99	5.95	95.39	-0.055	叶绿体 Chlo
PaPP2C56	FUN_037105-T2	518	57 007.52	5.26	93.44	-0.065	叶绿体 Chlo
PaPP2C57	FUN_037109-T1	397	44 043.49	4.81	95.53	-0.063	细胞核 Nucl
PaPP2C58	FUN_037397-T1	352	38 049.98	6.37	81.76	-0.382	叶绿体 Chlo
PaPP2C59	FUN_037631-T1	324	43 682.53	4.58	90.31	-0.263	细胞质 Cyto
PaPP2C60	FUN_038803-T1	397	44 146.12	8.44	88.52	-0.296	细胞核 Nucl
PaPP2C61	FUN_038803-T2	283	30 877.72	7.20	88.47	-0.300	叶绿体 Chlo
PaPP2C62	FUN_038943-T1	382	42 478.4	7.04	82.53	-0.277	叶绿体 Chlo
PaPP2C63	FUN_039200-T1	282	30 814.64	8.67	78.16	-0.320	叶绿体 Chlo
PaPP2C64	FUN_039354-T1	371	40 351.93	6.27	86.21	-0.281	叶绿体 Chlo
PaPP2C65	FUN_039354-T2	347	37 804.29	5.16	81.81	-0.170	叶绿体 Chlo
PaPP2C66	FUN_039387-T1	384	41 605.13	5.31	90.08	-0.147	叶绿体 Chlo
PaPP2C67	FUN_040333-T1	366	40 043.93	5.06	85.89	-0.303	叶绿体 Chlo
PaPP2C68	FUN_027600-T1	300	32 800.15	5.60	76.85	-0.236	叶绿体 Chlo
PaPP2C69	FUN_027658-T1	390	43 288.57	7.76	76.85	-0.236	叶绿体 Chlo
PaPP2C70	FUN_028496-T1	360	40 378.75	4.97	81.63	-0.418	叶绿体 Chlo
PaPP2C71	FUN_029922-T1	381	42 358.23	8.52	87.98	-0.302	叶绿体 Chlo
PaPP2C72	FUN_029922-T2	1 064	119 890.36	5.72	90.83	-0.130	叶绿体 Chlo
PaPP2C73	FUN_029973-T1	326	36 003.06	7.15	87.98	-0.302	细胞质 Cyto
PaPP2C74	FUN_030294-T1	632	69 772.38	8.68	88.87	-0.324	线粒体 Plas
PaPP2C75	FUN_030459-T1	400	44 247.33	5.41	79.61	-0.238	叶绿体 Chlo
PaPP2C76	FUN_031081-T1	628	69 390.53	5.73	83.15	-0.318	叶绿体 Chlo

甜樱桃PP2C家族全基因组鉴定与表达分析

Genomic-wide identification and expression analysis of the PP2C gene family in sweet cherry

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