›› 2010, Vol. 22 ›› Issue (1): 0-86.

• 论文 •    

SDS,Cu2+污染对蟾蜍蝌蚪的急性毒性及肝脏Na+-K+-ATPase活性的影响

金叶飞,祝尧荣,韩丽   

  1. 绍兴文理学院 生命科学学院,浙江 绍兴 312000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Acute toxicity of SDS, Cu2+ pollution and its effect on Na+-K+-ATPase activity in the liver of Bufo gargarizans

JIN Ye-fei;ZHU Yao-rong;HAN Li   

  1. College of Life Sciences, Shaoxing University, Shaoxing 312000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-25 Published:2010-01-25

摘要: 以中华大蟾蜍(Bufo gargarizans)蝌蚪为试验材料,采用联合指数相加法,研究了阴离子表面活性剂十二烷基硫酸钠(SDS)、重金属Cu2+对蟾蜍蝌蚪的急性毒性和联合毒性效应;并在急性毒性试验的基础上,研究了亚急性条件下SDS,Cu2+及其复合污染对蝌蚪肝脏Na+-K+-ATPase活性的影响。试验结果表明,SDS对蝌蚪的48,72,96 h的半致死浓度(LC50)分别为27.38,26.45,25.57 mg/L;Cu2+对蝌蚪的48,72,96 h的半致死浓度(LC50)分别为0.475,0.323,0.285 mg/L。SDS,Cu2+对蝌蚪的联合毒性作用48 h内表现为毒性增强的协同效应,96 h后表现为拮抗作用。蝌蚪在亚急性浓度的SDS,Cu2+条件下暴露4 d后,SDS,Cu2+及复合污染均对蝌蚪肝脏的Na+-K+-ATPase活性产生明显影响,SDS,Cu2+复合污染在Na+-K+-ATPase水平上表现为一定的协同作用。

关键词: 铜, 十二烷基硫酸钠, 中华大蟾蜍蝌蚪, 急性毒性, 联合毒性, Na+-K+-ATP酶

Abstract: To investigate the effects of single or combined sodium dodecyl sulfate (SDS) and Cu2+ pollution in Bufo gargarizans, the acute toxicity and joint toxicity tests of SDS, Cu2+ and Na+-K+-ATPase activity in liver under the subacute toxicities of single or combined SDS and Cu2+ were measured by using exposure experiment. The results showed that the LC50 of SDS for 48, 72 and 96 h to Bufo gargarizans were 27.38, 26.45 and 25.57 mg·L-1, respectively, and the LC50 of Cu2+ for 48, 72 and 96 h were 0.475, 0.323 and 0.285 mg·L-1, respectively. The toxicity of Cu2+ was higher than that of SDS. In addition, it also indicated that the joint toxicity of Cu2+ and SDS was synergistic at 48 h but antagonistic at 96 h. The results also indicated that single or combined treatments with Cu2+ and SDS could affect the Na+-K+-ATPase activity in liver of Bufo gargarizans, and the joint effect of the combined pollution on the Na+-K+-ATPase activity was synergistic.

Key words: copper, SDS, Bufo gargarizans, acute toxicity, joint toxicity, Na+-K+-ATPase