浙江农业学报

›› 2010, Vol. 22 ›› Issue (2): 0-160.

• 论文 •    

番茄种质遗传多样性的AFLP分析

姚祝平，叶青静，杨悦俭*，王荣青，阮美颖，周国治   

  1. 浙江省农业科学院　蔬菜研究所，浙江　杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25

Genetic diversity of tomato by AFLP analysis

YAO Zhu-ping;YE Qing-jing;YANG Yue-jian*;WANG Rong-qing;RUAN Mei-ying;ZHOU Guo-zhi   

  1. Institute of Vegetables，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

PDF (PC)

1450

被引次数

13

摘要： 利用AFLP技术对44份番茄材料进行遗传多样性和亲缘关系分析。从64对引物组合中筛选出15对用于扩增，共获得601条带，其中多态性带364条，多态性为60.5%，这表明供试材料在DNA水平上酶切位点的分布存在广泛的变异。利用UPGMA法将44份番茄材料分成5个复合组和1个独立组，从相似性系数分析了各材料之间的亲缘关系。

关键词: 番茄, AFLP, 遗传多样性, 亲缘关系

Abstract: The genetic diversity and relationship of 44 tomato accessions were analyzed by AFLP （amplified fragment length polymorphism）. Fifteen primer combinations selected from 64 primer combinations produced a total of 601 fragments，364 of which were polymorphic with a polymorphism percentage of 60.5%. This results showed that there were abundant diversities in enzyme digestion sites among different tomato varieties. These tomato germplasm resources were clustered into 5 multiple-member groups and 1 one-member group by UPGMA （unweighted pair group method with arithmetic average） based on Nei’s genetic distances. The genetic relationship of 44 tomato accessions was analyzed using the different coefficient.

Key words: tomato, AFLP, genetic diversity, relationship

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