pH值与离子效应对草菇生物量与IAA合成的调控

doi:10.3969/j.issn.1004-1524.20250337

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (4): 669-679.DOI: 10.3969/j.issn.1004-1524.20250337

pH值与离子效应对草菇生物量与IAA合成的调控

卯明娟¹^,²(), 普柒牛¹^,², 侯立娟¹^,²^,^*(), 马林¹, 蒋宁¹, 林金盛¹, 曲绍轩¹, 李辉平¹, 徐平¹, 刘迪²

¹ 江苏省农业科学院蔬菜研究所, 江苏省高校园艺作物遗传改良重点实验室, 江苏南京 210014
² 延边大学农学院, 吉林延吉 133002

收稿日期:2025-04-25 出版日期:2026-04-25 发布日期:2026-05-08
作者简介:*侯立娟,E-mail: mybailinggu@126.com
卯明娟,研究方向为食药用菌资源开发与利用。E-mail:Maomj02@163.com
通讯作者: 侯立娟
基金资助:
国家自然科学基金青年科学基金(31901933)

Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects

MAO Mingjuan¹^,²(), PU Qiniu¹^,², HOU Lijuan¹^,²^,^*(), MA Lin¹, JIANG Ning¹, LIN Jinsheng¹, QU Shaoxuan¹, LI Huiping¹, XU Ping¹, LIU Di²

¹ Jiangsu Key Laboratory of Horticultural Crops Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
² College of Agriculture, Yanbian University, Yanji 133002, Jilin, China

Received:2025-04-25 Published:2026-04-25 Online:2026-05-08
Contact: HOU Lijuan

摘要/Abstract

摘要：

外源乙酸钠(NaAc)可促进草菇原基期吲哚-3-乙酸(IAA)的合成,进而促进原基分化并提高产量,但其具体作用机制尚未阐明。本研究旨在分析NaAc诱导IAA合成的能力,采用深层培养方法,设计5个pH值梯度(6、7、8、9、10)、4种阴离子(Ac^-、HCO₃^-、C₅H₇O₅COO^-、H₂PO₄^-)和4种阳离子(Na⁺、Mg²⁺、Mn²⁺、K⁺)的三因素试验,探究添加NaAc对菌丝生物量与IAA合成的影响,为后续揭示NaAc调控IAA合成的机理提供理论基础。结果表明,在pH值为8条件下,菌丝生物量、乙醛脱氢酶(ALDH)活性,以及IAA、吲哚-3-乙醛(IAAld)、吲哚-3-丙酮酸(IPyA)、色胺(TAM)和吲哚-3-乙醛肟(IAOx)的含量均显著(p<0.05)提高,分别提高37.98%、58.39%、61.29%、58.33%、31.25%、29.23%和64.71%。在阴离子处理中,乙酸根离子(Ac^-)显著提高了生物量、IAA含量和ALDH活性,分别提高6.28%、22.58%和21.01%,IPyA、TAM和IAOx含量也显著高于对照。在阳离子处理中,Na⁺能显著促进生物量、IAA含量和ALDH活性,分别提高6.28%、22.58%和20.97%,且IPyA、TAM和IAOx含量也显著高于对照。说明Na⁺对IAA合成的促进作用优于Mg²⁺、Mn²⁺和K⁺。综合分析表明,在草菇营养生长阶段,添加NaAc可使培养环境pH值维持在适宜生长的碱性范围。pH值作为NaAc解离后的主要调控因子,与Ac^-和Na⁺共同影响草菇生长阶段的生物量积累和IAA合成。

关键词: 草菇, pH值, 离子效应, IAA合成, 生物量

Abstract:

Exogenous sodium acetate (NaAc) can promote indole-3-acetic acid (IAA) synthesis during the primordium stage of Volvariella volvacea, thereby promoting primordium differentiation and increasing yield, but its specific mechanism of action has not been elucidated. This study aimed to analyze the ability of NaAc to induce IAA synthesis. Using a submerged culture method, a three-factor experiment was designed with five pH value gradients(6, 7, 8, 9, 10), four anions (Ac^-, HCO₃^-, C₅H₇O₅COO^-, H₂PO₄^-), and four cations (Na⁺, Mg²⁺, Mn²⁺, K⁺) to investigate the effects of adding NaAc on mycelial biomass and IAA synthesis, providing a theoretical basis for subsequently revealing the mechanism by which NaAc regulates IAA synthesis. The results showed that under pH value 8 conditions, mycelial biomass, aldehyde dehydrogenase (ALDH) activity, and the contents of IAA, indole-3-acetaldehyde (IAAld), indole-3-pyruvic acid (IPyA), tryptamine (TAM) and indole-3-acetaldoxime (IAOx) were significantly (p<0.05) increased by 37.98%, 58.39%, 61.29%, 58.33%, 31.25%, 29.23% and 64.71%, respectively. Among the anions, acetate ions (Ac^-) significantly increased biomass, IAA content, and ALDH activity by 6.28%, 22.58%, and 21.01%, respectively, the contents of IPyA, TAM, and IAOx were also significantly higher than the control. Among the cations, Na⁺ significantly increased biomass, IAA content, and ALDH activity by 6.28%, 22.58% and 20.97%, respectively, and the contents of IPyA, TAM, and IAOx were also significantly higher than the control. This indicated that Na⁺ was superior to Mg²⁺, Mn²⁺, and K⁺ in promoting IAA synthesis. Comprehensive analysis showed that during the vegetative growth stage of V. volvacea, adding NaAc could maintain the culture environment pH value within an alkaline range suitable for growth. pH value, as the main regulatory factor after NaAc dissociation, together with Ac^- and Na⁺, influenced biomass accumulation and IAA synthesis during the growth stage of V. volvacea.

Key words: Volvariella volvacea, pH value, ionic effect, IAA synthesis, biomass

中图分类号:

S646.13

卯明娟, 普柒牛, 侯立娟, 马林, 蒋宁, 林金盛, 曲绍轩, 李辉平, 徐平, 刘迪. pH值与离子效应对草菇生物量与IAA合成的调控[J]. 浙江农业学报, 2026, 38(4): 669-679.

MAO Mingjuan, PU Qiniu, HOU Lijuan, MA Lin, JIANG Ning, LIN Jinsheng, QU Shaoxuan, LI Huiping, XU Ping, LIU Di. Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects[J]. Acta Agriculturae Zhejiangensis, 2026, 38(4): 669-679.

图/表 6

图1 初始pH值对草菇菌丝生长与生物量的影响柱上无相同小写字母表示在p<0.05水平上存在显著差异。下同。

Fig.1 Effect of initial pH value on mycelial growth and biomass of Volvariella volvacea Different lowercase letters above the bars indicate significant differences at the p<0.05 level. The same as below.

图2 不同初始pH值对草菇IAA合成关键产物和乙醛脱氢酶活性的影响 IAA,吲哚-3-乙酸;TAM,色胺;IPyA,吲哚-3-丙酮酸;IAAld,吲哚-3-乙醛;IAOx,吲哚-3-乙醛肟;ALDH,醛脱氢酶。下同。

Fig.2 Effects of different initial pH values on the key products of IAA synthesis and the activity of aldehyde dehydrogenase in Volvariella volvacea IAA, Indole-3-acetic acid; TAM, Tryptamine; IPyA, Indole-3-pyruvate; IAAld, Indole-3-acetaldehyde; IAOx, Indole-3-acetaldehyde oxime; ALDH, Aldehyde dehydrogenase. The same as below.

图3 不同阴离子对草菇菌丝生长和生物量的影响

Fig.3 Effects of different anions on mycelial growth and biomass of Volvariella volvacea

图4 不同阴离子对草菇IAA合成途径关键产物含量和乙醛脱氢酶活性的影响

Fig.4 Effects of different buffers on the key products of IAA synthesis pathway and the activity of aldehyde dehydrogenase in Volvariella volvacea

图5 不同阳离子对草菇菌丝生长和生物量的影响

Fig.5 Effects of different cations on mycelial growth and biomass of Volvariella volvacea

图6 不同阳离子对草菇IAA合成途径关键产物含量和乙醛脱氢酶活性的影响

Fig.6 Effects of different cations on the contents of key products of the IAA synthesis pathway and the activity of aldehyde dehydrogenase in Volvariella volvacea

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pH值与离子效应对草菇生物量与IAA合成的调控

Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects

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