浙江农业学报 ›› 2026, Vol. 38 ›› Issue (8): 1517-1528.DOI: 10.3969/j.issn.1004-1524.20250285

• 作物科学 • 上一篇    下一篇

35份贵州茶树种质资源的生化成分多样性分析与综合评价

郭维1(), 马贤森1, 聂红艳1, 宋治豪1, 周轶1, 陈光勋1, 陈正武2,*()   

  1. 1 毕节市农业科学院 茶叶果树研究所, 贵州 毕节 551700
    2 贵州省茶叶研究所, 贵州 贵阳 550006
  • 收稿日期:2025-04-07 出版日期:2026-08-25 发布日期:2026-09-14
  • 作者简介:郭维,研究方向为茶树育种与栽培。E-mail:243958897@qq.com
  • 通讯作者: *陈正武,E-mail:zwchentea@163.com
  • 基金资助:
    毕节市农业科学院科学技术项目(毕农科合〔2025〕3号)

Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province, China

GUO Wei1(), MA Xiansen1, NIE Hongyan1, SONG Zhihao1, ZHOU Yi1, CHEN Guangxun1, CHEN Zhengwu2,*()   

  1. 1 Tea and Fruit Research Institute, Bijie Academy of Agricultural Sciences, Bijie 551700, Guizhou, China
    2 Guizhou Tea Research Institute, Guiyang 550006, China
  • Received:2025-04-07 Published:2026-08-25 Online:2026-09-14

摘要:

为探明部分地方群体种和黄金芽自然杂交后代生化品质特点,为今后的茶树新品种选育工作提供参考,采用高效液相色谱法对贵州省茶叶研究所资源圃内35份茶树材料的28种生化成分进行检测,并运用遗传多样性分析、相关分析、主成分分析和聚类分析对这些种质资源进行综合评价。结果显示,28种生化成分的平均变异系数为43.04%,最大的是半胱氨酸含量(117.40%),最小的是咖啡碱含量(14.55%),遗传多样性指数平均值为1.81,最大的是苯丙氨酸含量(2.09),最小的是没食子儿茶素没食子酸酯含量(0.66),氨基酸类物质的遗传多样性指数均较高。主成分分析提取7个主成分,累计方差贡献率为78.451%,其中,总儿茶素、精氨酸、表没食子儿茶素没食子酸酯、谷氨酸、表儿茶素没食子酸酯、茶氨酸含量为关键指标。聚类分析将供试种质材料分为4类,第Ⅳ类(高氨基酸、低生物碱与儿茶素)综合评分最优,适宜加工名优绿茶。结合评价结果,筛选出GT-AS-70、GT-AS-83等9份材料作为培育绿茶适制品种的种质资源基础。

关键词: 茶树资源, 生化成分, 遗传多样性, 主成分分析, 聚类分析

Abstract:

To explore the quality characteristics of biochemical components in natural hybrid progenies derived from several local landraces and tea cultivar Huangjinya, and to supply theoretical references for future novel tea cultivar breeding, high-performance liquid chromatography (HPLC) was adopted to quantify 28 biochemical components across 35 tea accessions conserved in the germplasm nursery of Guizhou Tea Research Institute. Multiple statistical approaches including genetic diversity analysis, correlation analysis, principal component analysis (PCA) and cluster analysis were integrated to conduct a comprehensive evaluation of all tested accessions. The results revealed that the average coefficient of variation (CV) of the 28 biochemical components reached 43.04%. Cysteine (Cys) content exhibited the maximum CV of 117.40%, while caffeine (CAF) content showed the minimum CV of 14.55%. The mean value of the genetic diversity index was 1.81; phenylalanine (Phe) content possessed the highest index value of 2.09, and gallocatechin gallate (GCG) content had the lowest value of 0.66. Overall, amino acid components presented relatively high genetic diversity indexes. Seven principal components were extracted via PCA, accounting for a cumulative variance contribution rate of 78.451%. Total catechins (TC), arginine (Arg), epigallocatechin gallate (EGCG), glutamic acid (Glu), epicatechin gallate (ECG) and theanine (The) contents were identified as the core evaluation indicators. All tested accessions were divided into 4 groups by cluster analysis. Group Ⅳ, characterized by high amino acid contents as well as low alkaloid and catechin levels, obtained the optimal comprehensive score and possessed excellent suitability for manufacturing premium green tea. Based on the integrated evaluation results, 9 elite accessions represented by GT-AS-70 and GT-AS-83 were screened for developing green tea-specific cultivars.

Key words: tea plant resource, biochemical component, genetic diversity, principal component analysis, cluster analysis

中图分类号: