Detection and analysis of an egg cell-expressed gene in Nicotiana tabacum

doi:10.3969/j.issn.1004-1524.2017.03.02

›› 2017, Vol. 29 ›› Issue (3): 360-365.DOI: 10.3969/j.issn.1004-1524.2017.03.02

• Crop Science • Previous Articles Next Articles

Detection and analysis of an egg cell-expressed gene in Nicotiana tabacum

LUO An

College of Life Science, Yangtze University, Jingzhou 434023, China

Received:2016-07-28 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: cDNA pools of the egg cell and zygote of tobacco were constructed, and suppression subtractive hybridization and mirror orientation selection were used to select the genes specially expressed in the egg cell. So a gene named NtEE1 was found, which was CASP-like gene. NtEE1 encoded 194 amino-acid residues, in which 4 transmembrane domains existed by bioinformatics prediction. The subcellular localization of NtEE1-GFP also indicated that NtEE1 might be located on the nuclear membrane and plasma membrane. The promoter of actin11 of Arabidopsis was chosen to express nuclear-located GFP. The vector was transformed to tobacco, and the promoter activity of actin11 was proved to be intensive in the tobacco egg cell. These would provide a foothold to investigate the function of NtEE1 in the development of egg cell and the mechanism of gamete interaction in tobacco.

Key words: egg cell, transmembrane domain, tobacco, gamete interaction

CLC Number:

S572

LUO An. Detection and analysis of an egg cell-expressed gene in Nicotiana tabacum[J]. , 2017, 29(3): 360-365.

References

[1] NOWACK M K, GRINI P E, JAKOBY M J, et al. A positive signal from the fertilization of the egg cell sets off endosperm proliferation in angiosperm embryogenesis[J]. Nature Genetics , 2006, 38(1): 63-67.
[2] GROSS-HARDT R, KAGI C, BAUMANN N, et al. LACHESIS restricts gametic cell fate in the female gametophyte of Arabidopsis[J]. PloS Biology , 2007, 5(3): e47.
[3] PAGNUSSAT G C, YU H J, SUNDARESAN V. Cell-fate switch of synergid to egg cell in Arabidopsis eostre mutant embryo sacs arises from misexpression of the BEL1-like homeodomain gene BLH1[J]. Plant Cell , 2007, 19(11): 3578-3592.
[4] CHEN Z, TAN J L, INGOUFF M, et al. Chromatin assembly factor 1 regulates the cell cycle but not cell fate during male gametogenesis in Arabidopsis thaliana [J]. Development , 2008, 135(1): 65-73.
[5] INGOUFF M, SAKATA T, LI J, et al. The two male gametes share equal ability to fertilize the egg cell in Arabidopsis thaliana [J]. Current Biology , 2009, 19(1): 19-20.
[6] CASS D D. An ultrastructural and Nomarski-interference study of the sperms of barley[J]. Canadian Journal of Botany , 1973, 51(3): 601-605.
[7] 胡适宜, 李乐工, 朱激. 烟草生活胚囊及胚囊原生质体的分离[J].植物学报, 1985, 27(4): 337-344.
HU S Y, LI L G, ZHU J. Isolation of viable embryo sacs and their protoplasts of Nicotiana tabacum [J]. Acta Botanica Sinica , 1985, 27(4): 337-344. (in Chinese with English abstract)
[8] SPRUNCK S, BAUMANN U, EDWARDS K, et al. The transcript composition of egg cells changes significantly following fertilization in wheat ( Triticum aestivum L.)[J]. Plant Journal , 2005, 41(5): 660-672.
[9] DOMOKI M, SZUCS A, JAGER K, et al. Identification of genes preferentially expressed in wheat egg cells and zygotes[J]. Plant Cell Reports , 2013, 32(3): 339-348.
[10] ZHAO J, XIN H, QU L, et al. Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote, and mark the onset of the maternal-to-zygotic transition[J]. Plant Journal , 2011, 65(1): 131-145.
[11] XIN H P, PENG X B, NING J, et al. Expressed sequence-tag analysis of tobacco sperm cells reveals a unique transcriptional profile and selective persistence of paternal transcripts after fertilization[J]. Plant Reproduction , 2011, 24(1): 37-46.
[12] WUEST S E, VIJVERGERG K, SCHMIDT A, et al. Arabidopsis female gametophyte gene expression map reveals similarities between plant and animal gametes[J]. Current Biology , 2010, 20(6): 506-512.
[13] ANDERSON S N, JOHNSON C S, JONES D S, et al. Transcriptomes of isolated Oryza sativa gametes characterized by deep sequencing: evidence for distinct sex-dependent chromatin and epigenetic states before fertilization[J]. Plant Journal , 2013, 76(5): 729-741.
[14] ABIKO M, MAEDA H, TAMURA K, et al. Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up-or down-regulated genes in zygotes after fertilization[J]. Journal of Experimental Botany , 2013, 64(7): 1927-1940.
[15] OHNISHI T, TAKANASHI H, MOGI M, et al. Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays[J]. Plant Physiology , 2011, 155(2): 881-891.
[16] RUSSELL S D, GOU X, WONG C E, et al. Genomic profiling of rice sperm cell transcripts reveals conserved and distinct elements in the flowering plant male germ lineage[J]. New Phytologist , 2012, 195(3): 560-573.
[17] 孙蒙祥, 杨宏远, 周嫦. 分离烟草胚囊的新方法及诱导卵细胞与助细胞原生质体的原位融合[J].植物学报, 1993, 35(12): 893-900.
SUN M X, YANG H Y, ZHOU C. A new method for embryo sac isolation and in situ fusion of egg and synergid protoplasts in Nicotiana tabacum [J]. Acta Botanica Sinica , 1993, 35(12): 893-900. (in Chinese with English abstract)
[18] 傅春梅, 孙蒙祥, 周嫦, 等. 烟草受精后胚囊和合子的分离及合子的离体分裂[J].植物学报, 1996, 38(4):262-267.
FU C M, SUN M X, ZHOU C, et al. Isolation of fertilized embryo sacs and zygotes and triggering of zygote division in vitro in Nicotiana tabacum [J]. Acta Botanica Sinica , 1996, 38(4):262-267. (in Chinese with English abstract)
[19] REBRIKOV D V, BRITANOVA O V, GURSKAYA N G, et al. Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization[J]. Nucleic Acids Research , 2000, 28(20):E90.
[20] QU L J, LI L, LAN Z, et al. Peptide signaling during the pollen tube journey and double fertilization[J]. Journal of Experimental Botany , 2015, 66(17):5139-5150.
[21] MORI T, IGAWA T, TAMIYA G, et al. Gamete attachment requires GEX2 for successful fertilization in Arabidopsis[J]. Current Biology , 2014, 24(2):170-175.
[22] SPRUNCK S, RADEMACHER S, VOGLER F, et al. Egg cell-secreted EC1 triggers sperm cell activation during double fertilization[J]. Science , 2012, 338(6110): 1093-1097.

Detection and analysis of an egg cell-expressed gene in Nicotiana tabacum

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