Cloning and expression analysis of potassium channel NtTPK gene in tobacco

doi:10.3969/j.issn.1004-1524.2017.03.03

›› 2017, Vol. 29 ›› Issue (3): 366-372.DOI: 10.3969/j.issn.1004-1524.2017.03.03

• Crop Science • Previous Articles Next Articles

Cloning and expression analysis of potassium channel NtTPK gene in tobacco

XU Li, HUANG Luping, LU Liming, LI Liqin^*

College of Agronomy, Sichuan Agricultural University, Chengdu 625014, China

Received:2016-10-28 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: A new potassium channel gene NtTPK was cloned from cultivar K326 using homologous cloning strategy. Sequences analysis showed this gene contained 1 317 bp and coded 438 amino acid residues. Quantitative real-time PCR results showed that the highest expression level of this gene was in the stem; and the lowest expression level emerged after 24 h under low potassium treatment; the gene expression reached its peak level after 24 h under 200 mmol·L^-1 NaCl treatment; and 7 d after topping, the gene expression showed the highest level in leaves and roots of tobacco. These results provide theoretical bases for further study of the function of potassium channel NtTPK gene.

Key words: tobacco, potassium channel, NtTPK, bioinformatics, gene expression

CLC Number:

S572

XU Li, HUANG Luping, LU Liming, LI Liqin. Cloning and expression analysis of potassium channel NtTPK gene in tobacco[J]. , 2017, 29(3): 366-372.

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Cloning and expression analysis of potassium channel NtTPK gene in tobacco

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