Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T3AS·3AL-7Lr#1S

doi:10.3969/j.issn.1004-1524.2019.04.02

›› 2019, Vol. 31 ›› Issue (4): 519-524.DOI: 10.3969/j.issn.1004-1524.2019.04.02

• Crop Science • Previous Articles Next Articles

Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T3AS·3AL-7Lr#1S

ZHANG Yali^1,2, WANG Linsheng^1,2,*

1.College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China;
2. Luoyang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation, Luoyang 471023, China

Received:2018-04-01 Online:2019-04-25 Published:2019-04-19

Abstract

Abstract: Leymus racemosus is highly resistant to wheat scab (Fusarum head blight, FHB). The transfer of scab resistant gene from L. racemosus to Triticum aestivum is of great significance for broadening the base of wheat resistance. In the present study, the pollen of T. aestivum-L. racemosus disomic addition line DA7Lr with scab resistance was irradiated by⁶⁰Co-γ-rays 1 200 R (100 R·min^-1) prior to pollinating to emasculated T. aestivum cv. Chinese Spring. One plant with a translocated chromosome was detected in the M₁ by GISH. The plant with one translocation chromosome was self-pollinated, and at meiotic metaphase Ⅰ its progenies with two translocation chromosomes were analyzed for chromosome pairing behavior in their pollen mother cells (PMCs). One ring bivalent was observed, indicating that the plant with two translocation chromosomes was one translocation homozygote. C-banding and sequential GISH/FISH analysis, using Oligo-pAs1-2 and Oligo-pSc119.2-2 as probe, translocation line T3AS·3AL-7Lr#1S was confirmed. Three molecular markers of EST-STS such as BE591127, BQ168298 and BE591737 were screened, and these markers can be used to track the translocation line. The translocation line had good resistance to wheat scab and feasibility to be used as a new germplasm in wheat breeding resistant to scab disease.

Key words: Triticum aestivum, Leymus racemosus, translocation line, molecular and cytogenetics

CLC Number:

S512

ZHANG Yali, WANG Linsheng. Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T3AS·3AL-7Lr#1S[J]. , 2019, 31(4): 519-524.

References

[1] 李韬,李嫒嫒,李磊. 小麦赤霉病:从表型鉴定到抗性改良[J]. 科技导报,2016,34(22):75-80.
LI T, LI A A, LI L.Fusarium head blight in wheat: From phenotyping to resistance improvement[J]. Science Technology Review, 2016, 34(22):75-80. (in Chinese)
[2] BAI G H, SHANER G.Management and resistance in wheat and barley to fusarium head blight[J]. Annual Review of Phytopathology, 2004,42(1): 135-161.
[3] PESTKA J J.Deoxynivalenol: mechanisms of action, human exposure,and toxicological relevance[J]. Archives of Toxicology, 2010, 84(9): 663-679.
[4] ZHANG J B, WANG J H, GONG A D, et al.Natural occurrence of fusarium head blight, mycotoxins and mycotoxin-producing isolates of Fusariumin commercial fields of wheat in Hubei[J]. Plant Pathology, 2013, 62(1): 92-102.
[5] 刘大钧. 小麦抗赤霉病育种一个世界性难题[M]//中国农学会. 21世纪小麦遗传育种展望-小麦遗传育种国际学术讨论会文集. 北京: 中国农业科技出版社, 2001: 4-12.
[6] MCGUIRE P E, DVÔRÁK J. High salt-tolerance potential in wheatgrasses[J]. Crop Science, 1981, 21(5): 702-705.
[7] MUJEEB-KAZI A, BEKELE G T, MIRAND J L.Incorporation of alien genetic information from Elymus giganteus into Triticum aestivum[C]// Proceedings of the 6th International Wheat Genetics Symposium. Kyoto, Japan, 1983. 223-231.
[8] 陈佩度,王兆悌,王苏玲,等.将大赖草种质转移给普通小麦的研究:Ⅲ.抗赤霉病异附加系选育[J]. 遗传学报, 1995, 22(3): 206-210.
CHEN P D, WANG Z T, WANG S L, et al.Transfer of useful germplasm from Leymus racemosus Lam. to common wheat. Ⅲ. Development of addition lines with wheat scab resistance[J]. Acta Genetica Sinica, 1995, 22(3): 206-210. (in Chinese with English abstract)
[9] WANG L S, CHEN P D.Development of Triticum aestivum-Leymus racemosus ditelosomic substitution line 7Lr#1S(7A) with resistance to wheat scab and its meiotic behavior analysis[J]. Chinese Science Bulletin, 2008, 53(22): 3522-3529.
[10] QI L L, PUMPHREY M O, FRIEBE B, et al.Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat[J]. Theoretical and Applied Genetics, 2008, 117(7): 1155-1166.
[11] 刘文轩,陈佩度,刘大钧. 利用减数分裂期成株电离辐射选育小麦-大赖草易位系的研究[J]. 植物学报,1999, 41(5):463-467.
LIU W X, CHEN P D, LIU D J.Development of Triticum aestivum-Leymus racemosus translocation lines by irradiating adult plants at meiosis[J]. Acta Botanica Sinica, 1999, 41(5): 463-467. (in Chinese with English abstract)
[12] WANG L S, CHEN P D,WANG X E.Molecular cytogenetic analysis of Triticum aestivum-Leymus racemosus reciprocal chromosomal translocation T7DS·5LrL/T5LrS·7DL[J]. Chinese Science Bulletin, 2010, 55(11): 1026-1031.
[13] 崔承齐,王林生,陈佩度. 普通小麦-大赖草易位系T7BS·7Lr#1S和T2AS·2AL-7Lr#1S的分子细胞遗传学鉴定[J]. 作物学报, 2013,39(2):191-197.
CUI C Q, WANG L S, CHEN P D.Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation lines T7BS·7Lr#1S and T2AS·2AL-7Lr#1S[J]. Acta Agronomica Sinica, 2013, 39(2):191-197. (in Chinese with English abstract)
[14] GILL B S, FRIEBE B, ENDO T R.Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum)[J]. Genome, 1991, 34(5): 830-839.
[15] MUKAI Y, NAKAHARA Y, YAMAMOTO M.Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes[J]. Genome, 1993, 36(3): 489-494.
[16] ZHANG P, LI W L, FRIEBE B, et al.Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH[J]. Genome, 2004, 47(5): 979-987.
[17] TANG Z X, YANG Z J, FU S L.Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis[J]. Journal of Applied Genetics, 2014, 55(3): 313-318.
[18] SHARP P J, CHAO S, DESAI S, et al.The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm[J]. Theoretical and Applied Genetics, 1989, 78(3): 342-348.
[19] 王裕中,杨新宁,肖庆璞. 小麦赤霉病抗性鉴定技术的改进及其抗源的开拓[J]. 中国农业科学,1982(5):66-67.
WANG Y Z, YANG X N, XIAO Q P.The improvement of identification technique of scab(>Gibberella zeae Petch.) resistance of wheat and the development of resistant sources[J]. Scientia Agricultura Sinica, 1982(5): 66-67.
[20] MA H X, YAO J B, ZHOU M P, et al.Molecular breeding for wheat Fusarium head blight resistance in China[J]. Cereal Research Communications, 2008, 36(Suppl):203-212.
[21] ZHANG J, ZHANG J P, LIU W H, et al.An intercalary translocation from Agropyron cristatum 6P chromosome into common wheat confers enhanced kernel number per spike[J]. Planta, 2016, 244(4): 853-864.
[22] SONG L, LU Y, ZHANG J, et al.Physical mapping of Agropyron cristatum chromosome 6P using deletion lines in common wheat background[J]. Theoretical and Applied Genetics,2016 ,129(5):1023-1034.

Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T3AS·3AL-7Lr#1S

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Tangang, SUN Ting, WANG Jichuan, LI Huiqin, GAO Zhen, SHI Yuanqiang. Effects of sowing date and density on population structure and lodging resistance of winter wheat under drip irrigation [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 193-202.
[2]	BAO Lie, WANG Mantao, LIU Jiangchuan, WEN Bo, MING Yue. Estimation method of wheat yield based on convolution neural network [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2244-2252.
[3]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[4]	XU Li, CHEN Xiaojie, CAO Jingting, LIU Chuchu, DING Ting, JIANG Teng. Resistance mechanism of biocontrol strain DZSG23 against wheat scab [J]. , 2020, 32(11): 2001-2008.
[5]	BIAN Jianwen, CUI Yan, YANG Songqi, LUO Guanghong, MENG Xiangang. Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress [J]. , 2020, 32(10): 1748-1756.
[6]	HAN Lijie, DONG Weixin, ZHANG Yuechen. Effects of different water and fertilizer treatments on canopy structure, yield and grain quality of wheat [J]. , 2020, 32(6): 953-962.
[7]	XU Xiao, LUAN Haiye, ZHANG Yinghu, LU Jian, QIAO Hailong, ZANG Hui, YANG Hongyan, SHEN Huiquan. Morphological diversity of hulless barley accessions from Qinghai-Tibetan Plateau [J]. , 2019, 31(7): 1037-1044.
[8]	SHI Yujie, LI Xinglong, TANG Yuan, YU Haiping, WU Xiaoyong. Study on metabolic differences of white quinoa from two areas based on GC-MS [J]. , 2019, 31(6): 869-877.
[9]	MA Yanwen, GAO Huali, LI Qianwen, YU Junjie, LYU Zhiwei. Optimization of solid fermentation for enzymes produced by Aspergillar sulphureus SQ-3 [J]. , 2019, 31(6): 970-976.
[10]	WANG Zhangjun, LIU Yan, ZHANG Shuangxi, LIU Fenglou, LI Qingfeng, ZHANG Xiaogang, LIU Shengxiang, JIA Biao. Identification on disease resistance and molecular markers of F₂ hybrids from Ningchun No.4 and Hedong black wheat [J]. , 2019, 31(5): 677-687.
[11]	WANG Chunmei, GU Xingfa, YU Tao, ZHOU Xiang, ZHAN Yulin, HAN Leran, XIE Qiuxia. Recent advances of validation methods in passive microwave remote sensing of soil moisture products [J]. , 2019, 31(5): 846-854.
[12]	YAN Jianwei, SU Xiaodong, ZHAO Yuan, LIU Jinping. Wheat germ sheath recognition based on image features [J]. , 2019, 31(2): 326-332.
[13]	LIU Yong'an, PAN Binrong, YUE Gaohong, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region [J]. , 2018, 30(12): 2001-2006.
[14]	SHEN Yesong, WANG Xin, GU Zhengzhong, YANG Zibo, ZHAN Qiuwen. Quality analysis of 296 wheat varieties from the Huang-Huai wheat region planted in Huaibei area of Jiangsu [J]. , 2018, 30(10): 1617-1623.
[15]	DENG Tiantian, CHEN Xi, HOU Yumeng. Effects of combined symbiotic nitrogen-fixing bacteria on growth of wheat seed under different environmental factors [J]. , 2018, 30(6): 886-892.