Identification and expression analysis of cytochrome P450 family genes from Physalis angulata L.

doi:10.3969/j.issn.1004-1524.2021.11.02

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (11): 2009-2016.DOI: 10.3969/j.issn.1004-1524.2021.11.02

• Crop Science • Previous Articles Next Articles

Identification and expression analysis of cytochrome P450 family genes from Physalis angulata L.

JIANG Zhifang¹^,²(), HAN Yidie¹, LOU Panpan¹, GUO Hong¹, FENG Shangguo¹^,², SHEN Chenjia¹^,², WANG Huizhong1¹^,²^,^*()

1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
2. Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou 311121, China

Received:2020-12-22 Online:2021-11-25 Published:2021-11-26
Contact: WANG Huizhong1

Abstract

Abstract:

Cytochrome P450 enzymes are widely involved in plant secondary metabolism. Based on the transcriptome data of Physalis angulata, several P450 family members had been identified. 795 possible sequence fragments of P450 gene had been identified, and they were clustered into 25 different clusters, including 12 clustering with tissue-specific expression. By bioinformatics splicing, full-length sequence of 30 Physalis angulata P450 gene family, which was highly homologous with other P450 species genes, were obtained. Phylogenetic analysis showed that 30 P450 genes could be classified into nine P450 subfamilies. By qRT-PCR method, expression of 30 P450 genes under different developmental stages of fruits was analyzed. The expression of nine P450 genes increased with fruit maturation, while expression of other nine P450 genes decreased gradually. The expression of P450 family genes under ethylene (ACC) and ethylene inhibitor (1-methylcyclopropene, 1-MCP) treatments were also analyzed. Part of the P450 family genes showed the opposite expression pattern under ACC and 1-MCP treatments, suggesting P450 family genes might be involved in ethylene-mediated fruit ripening process. Further, three fruit-specific expressed P450 family genes (comp164210, comp131532, comp152181) had been cloned, and they were mainly located in the cytoplasm and cell membrane. This study provided valuable genetic information for the genetic improvement of medicinal characters of P. angulata.

Key words: cytochrome P450 enzyme, Physalis angulate, transcriptome, ethylene

CLC Number:

S567.21

JIANG Zhifang, HAN Yidie, LOU Panpan, GUO Hong, FENG Shangguo, SHEN Chenjia, WANG Huizhong1. Identification and expression analysis of cytochrome P450 family genes from Physalis angulata L.[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2009-2016.

Figures/Tables 7

Table 1 Primer sequence for P450 gene of Physalis angulata L.

P450基因 P450 gene	上游引物(5'→3') Forward primer sequence(5'→3')	下游引物(5'→3') Reverse primer sequence(5'→3')
comp164210	GGTACCATGGTAGATATTCATGGAT	GTCGACAGTGGATGATATTGTTGAT
comp131532	GGTACCATGGATTTCTCTCTTGCC	GTCGACTATATCGTTGTACAAATCTG
comp152181	GGTACCATGGTGCTTTCTCTTTTTTC	GTCGACACGGTGGCTTATTGGTTTC

Fig.1 Expression spectrum analysis of P450 genes in Physalis angulate Green meant low expression, red meant high expression.

Fig.2 Homologous and phylogenetic analysis of P450 genes

Fig.3 Expression level of P450 genes at different ripe stages of fruit

Fig.4 Expression analysis of the P450 family genes under ACC and 1-MCP treatments

Fig.5 Clone of three fruit-specific expression P450 genes

Fig.6 Subcellular localization of three P450 protein

References 23

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Identification and expression analysis of cytochrome P450 family genes from Physalis angulata L.

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