Effects of optimizing fertilization methods on rice yield and soil nutrient balance

doi:10.3969/j.issn.1004-1524.20240132

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (2): 438-446.DOI: 10.3969/j.issn.1004-1524.20240132

• Environmental Science • Previous Articles Next Articles

Effects of optimizing fertilization methods on rice yield and soil nutrient balance

LI Jianqiang¹(), WEI Qianqian², LIU Xiaoxia³, ZHANG Junhua², ZHU Chunquan²^,^*()

1. Pinghu Plant Protection and Soil Fertilizer Technology Promotion Center, Pinghu 314200, Zhejiang, China
2. State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
3. Zhejiang Cultivated Land Quality and Fertilizer Administration Station, Hangzhou 310020, China

Received:2024-02-19 Online:2025-02-25 Published:2025-03-20
Contact: ZHU Chunquan

Abstract

Abstract:

To explore suitable new fertilizers and the optimal application dosage in the Hangzhou-Jiahu-Hu region, three kinds of news fertilizers were used in present study. Taking the local conventional fertilizer application dosage in Pinghu as the control, the new types of fertilizer which suitable for the high quality and high yield rice production in the local were screened out by a field experiment, and further to explore the method with the purpose of nitrogen reduction and efficiency improvement for new fertilizer. The results showed that compared with the conventional fertilizer application in the local area, the application of Maoshi slow-release fertilizer significantly (P<0.05) increased rice dry weight and nitrogen use efficiency, and increased rice yield via increasing the seed numbers per spike and the seed setting rate. Furthermore, Maoshi slow-release fertilizer significantly increased soil organic matter content and alkali hydrolyzed nitrogen content, increased the activities of urease and ammonia monooxygenase along with abundance of relevant functional genes, and increased soil microbial biomass carbon and nitrogen content. Compared to the conventional nitrogen application amount, reduced 10% of nitrogen when applied Maoshi slow-release fertilizer significantly increased nitrogen use efficiency without compromising rice yield and rice quality. In summary, applying Moshi slow-release fertilizer with a 10% nitrogen reduction from conventional dosage achieves both nitrogen reduction and efficieny enhancement in rice cultivation.

Key words: Maoshi slow-release fertilizer, nitrogen reduction and efficiency enhancement, rice, nitrogen use efficiency, yield

CLC Number:

S511

LI Jianqiang, WEI Qianqian, LIU Xiaoxia, ZHANG Junhua, ZHU Chunquan. Effects of optimizing fertilization methods on rice yield and soil nutrient balance[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 438-446.

Figures/Tables 12

References 21

[1]	文平兰, 陈海波, 童星. 不同新型肥料在句容市小麦生产上的应用效果初探[J]. 上海农业科技, 2023(2): 141-143.
	WEN P L, CHEN H B, TONG X. Preliminary study on the application effect of different new fertilizers in wheat production in Jurong city[J]. Shanghai Agricultural Science and Technology, 2023(2): 141-143. (in Chinese)
[2]	丁文成, 何萍, 周卫. 我国新型肥料产业发展战略研究[J]. 植物营养与肥料学报, 2023, 29(2): 201-221.
	DING W C, HE P, ZHOU W. Development strategies of the new-type fertilizer industry in China[J]. Journal of Plant Nutrition and Fertilizers, 2023, 29(2): 201-221. (in Chinese with English abstract)
[3]	王文青, 何玮恒, 陈培峰, 等. 新型缓释肥料对水稻产量及氮素吸收利用的影响[J]. 江西农业学报, 2023, 35(1): 84-87.
	WANG W Q, HE W H, CHEN P F, et al. Effect of new slow-release fertilizer on rice yield and nitrogen uptake and utilization[J]. Acta Agriculturae Jiangxi, 2023, 35(1): 84-87. (in Chinese with English abstract)
[4]	刘永红, 郑文涛, 张晋天, 等. 缓/控释肥研究进展及其应用[J]. 华中农业大学学报, 2023, 42(4): 167-176.
	LIU Y H, ZHENG W T, ZHANG J T, et al. Progress and its application of slow/controlled release fertilizers in agricultural production[J]. Journal of Huazhong Agricultural University, 2023, 42(4): 167-176. (in Chinese with English abstract)
[5]	卢忠诚, 傅丽青, 黄其颖, 等. 缓释肥对水稻甬优1540产量及肥料利用率的影响[J]. 农技服务, 2022, 39(11): 1-4.
	LU Z C, FU L Q, HUANG Q Y, et al. Effect of slow-release fertilizer on yield and fertilizer utilization rate of rice Yongyou 1540[J]. Agricultural Technology Service, 2022, 39(11): 1-4. (in Chinese)
[6]	李虹儒, 许景钢, 徐明岗, 等. 我国典型农田长期施肥小麦氮肥回收率的变化特征[J]. 植物营养与肥料学报, 2009, 15(2): 336-343.
	LI H R, XU J G, XU M G, et al. Change characteristic of nitrogen recovery efficiency of wheat in typical farmland of China under long-term fertilization[J]. Plant Nutrition and Fertilizer Science, 2009, 15(2): 336-343. (in Chinese with English abstract)
[7]	WANG C, LV J, XIE J M, et al. Effect of slow-release fertilizer on soil fertility and growth and quality of wintering Chinese chives (Allium tuberm Rottler ex Spreng.) in greenhouses[J]. Scientific Reports, 2021, 11(1): 8070.
[8]	胡香玉, 钟旭华, 彭碧琳, 等. 减氮条件下高产水稻品种的产量形成和氮素利用特征[J]. 核农学报, 2019, 33(12): 2460-2471.
	HU X Y, ZHONG X H, PENG B L, et al. Yield formation and characteristics of nitrogen utilization in high-yielding rice varieties under reduced nitrogen input[J]. Journal of Nuclear Agricultural Sciences, 2019, 33(12): 2460-2471. (in Chinese with English abstract)
[9]	杜加银, 茹美, 倪吾钟. 减氮控磷稳钾施肥对水稻产量及养分积累的影响[J]. 植物营养与肥料学报, 2013, 19(3): 523-533.
	DU J Y, RU M, NI W Z. Effects of fertilization with reducing nitrogen, controlling phosphorus and stabilizing potassium on rice yield and nutrient accumulation[J]. Journal of Plant Nutrition and Fertilizer, 2013, 19(3): 523-533. (in Chinese with English abstract)
[10]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[11]	李振高, 骆永明, 滕应. 土壤与环境微生物研究法[M]. 北京: 科学出版社, 2008.
[12]	师恩慧. 蚯蚓在秸秆堆制过程中对氮代谢的影响[D]. 长春: 吉林大学, 2022.
	SHI E H. Effect of earthworm on nitrogen metabolism in straw composting process[D]. Changchun: Jilin University, 2022. (in Chinese with English abstract)
[13]	李晨华, 贾仲君, 唐立松, 等. 不同施肥模式对绿洲农田土壤微生物群落丰度与酶活性的影响[J]. 土壤学报, 2012, 49(3): 567-574.
	LI C H, JIA Z J, TANG L S, et al. Effect of model of fertilization on microbial abundance and enzyme activity in oasis farmland soil[J]. Acta Pedologica Sinica, 2012, 49(3): 567-574. (in Chinese with English abstract)
[14]	JIANG D Q, JIANG N, JIANG H, et al. Urease inhibitors increased soil ureC gene abundance and intracellular urease activity when extracellular urease activity was inhibited[J]. Geoderma, 2023, 430: 116295.
[15]	全国粮油标准化技术委员会. 优质稻谷:GB/T 17891—2007[S/OL]. [2024-02-18]. https://openstd.samr.gov.cn/bzgk/gb/newGbInfo?hcno=56C97D505F4880DE76E2D49C7DA0C872.
[16]	赵秉强, 袁亮. 化肥产品创新驱动产业转型升级[J]. 植物营养与肥料学报, 2022, 28(4): 726-731.
	ZHAO B Q, YUAN L. Innovation in chemical fertilizer products driving industrial transformation and evolution[J]. Journal of Plant Nutrition and Fertilizers, 2022, 28(4): 726-731. (in Chinese with English abstract)
[17]	胡启良, 杨滨娟, 刘宁, 等. 绿肥混播下不同施氮量对水稻产量、土壤碳氮和微生物群落的影响[J]. 华中农业大学学报, 2022, 41(6): 16-26.
	HU Q L, YANG B J, LIU N, et al. Effects of application rates of nitrogen on rice yield, carbon and nitrogen, microbial community in soil under mixed sowing of green manure[J]. Journal of Huazhong Agricultural University, 2022, 41(6): 16-26. (in Chinese with English abstract)
[18]	CONACHER J, CONACHER A. Organic farming and the environment, with particular reference to Australia: a review[J]. Biological Agriculture & Horticulture, 1998, 16: 145-171.
[19]	高菊生, 黄晶, 董春华, 等. 长期有机无机肥配施对水稻产量及土壤有效养分影响[J]. 土壤学报, 2014, 51(2): 314-324.
	GAO J S, HUANG J, DONG C H, et al. Effects of long-term combined application of organic and chemical fertilizers on rice yield and soil available nutrients[J]. Acta Pedologica Sinica, 2014, 51(2): 314-324. (in Chinese with English abstract)
[20]	张水清, 林杉, 郭斗斗, 等. 长期施肥下潮土全氮、碱解氮含量与氮素投入水平关系[J]. 中国土壤与肥料, 2017(6): 23-29.
	ZHANG S Q, LIN S, GUO D D, et al. Relationship between total nitrogen, alkali hydrolyzed nitrogen content and nitrogen input levels in fluvo-aquic soil under long term fertilization[J]. Soil and Fertilizer Sciences in China, 2017(6): 23-29. (in Chinese with English abstract)
[21]	熊淑萍, 吴延鹏, 王小纯, 等. 减氮处理对不同小麦品种干物质积累及氮素转运特性的影响[J]. 麦类作物学报, 2015, 35(8): 1134-1140.
	XIONG S P, WU Y P, WANG X C, et al. Effect of lower nitrogen application on dry matter accumulation and nitrogen translocation of different wheat varieties[J]. Journal of Triticeae Crops, 2015, 35(8): 1134-1140. (in Chinese with English abstract)

基因名称 Gene name	正向引物序列(5'→3') Forward primer sequence(5'→3')	反向引物序列(5'→3') Reverse primer sequence(5'→3')	参考文献 Reference
ureC	AAGSTSCACGAGGACTGGGG	AGGTGGTGGCASACCATSAGCAT	[14]
Arch-amoA	TAATGGTCTGGCTTAGACG	CGGCCATCCATCTGTATGT	[13]
Bact-amoA	GGGGTTTCTACTGGTGGT	CCCCTCKGSAAAGCCTTCTTC	[13]

基因名称 Gene name	正向引物序列(5'→3') Forward primer sequence(5'→3')	反向引物序列(5'→3') Reverse primer sequence(5'→3')	参考文献 Reference
ureC	AAGSTSCACGAGGACTGGGG	AGGTGGTGGCASACCATSAGCAT	[14]
Arch-amoA	TAATGGTCTGGCTTAGACG	CGGCCATCCATCTGTATGT	[13]
Bact-amoA	GGGGTTTCTACTGGTGGT	CCCCTCKGSAAAGCCTTCTTC	[13]

处理 Treatment	穗数 Spike number/(10⁴ hm^-2)	穗粒数 Seed number per spike	结实率 Seed setting rate/%	千粒重 1 000-gain weight/g	产量 Yield/(kg·hm^-2)
CK	206.10±4.80 c	190.78±1.59 c	96.47±0.37 a	23.24±0.02 b	9 094.80±215.55 c
CF	244.35±14.70 ab	184.69±1.15 d	85.92±1.26 c	23.30±0.05 a	9 455.40±195.75 b
MSF	244.50±7.80 ab	194.41±2.19 b	90.75±1.33 b	23.32±0.03 b	9 810.45±207.45 a
JSF	239.25±7.65 b	181.34±2.73 e	89.18±0.52 b	24.19±0.02 a	9 606.15±135.00 b
SSF	245.55±4.20 a	208.31±4.93 a	90.28±1.45 b	23.22±0.04 b	9 724.80±297.60 b

处理 Treatment	穗数 Spike number/(10⁴ hm^-2)	穗粒数 Seed number per spike	结实率 Seed setting rate/%	千粒重 1 000-gain weight/g	产量 Yield/(kg·hm^-2)
CK	206.10±4.80 c	190.78±1.59 c	96.47±0.37 a	23.24±0.02 b	9 094.80±215.55 c
CF	244.35±14.70 ab	184.69±1.15 d	85.92±1.26 c	23.30±0.05 a	9 455.40±195.75 b
MSF	244.50±7.80 ab	194.41±2.19 b	90.75±1.33 b	23.32±0.03 b	9 810.45±207.45 a
JSF	239.25±7.65 b	181.34±2.73 e	89.18±0.52 b	24.19±0.02 a	9 606.15±135.00 b
SSF	245.55±4.20 a	208.31±4.93 a	90.28±1.45 b	23.22±0.04 b	9 724.80±297.60 b

处理 Treatment	有机质含量 Organic matter content/%	总氮含量 Total nitrogen content/(g·kg^-1)	碱解氮含量 Alkali hydrolyzed content N/(mg·kg^-1)	有效磷含量 Available P content/(mg·kg^-1)	速效钾含量 Available K content/(mg·kg^-1)
CK	2.57±0.27 b	1.32±0.07 b	146.35±5.31 c	62.09±2.96 a	58.36±2.86 b
CF	2.54±0.13 b	1.33±0.05 ab	155.26±7.69 b	54.06±5.67 b	63.96±6.39 ab
MSF	3.08±0.21 a	1.42±0.08 a	167.28±8.72 a	55.92±4.83 b	68.52±4.13 ab
JSF	2.66±0.32 b	1.39±0.09 ab	151.82±12.73 b	68.31±8.04 a	72.03±9.22 a
SSF	2.48±0.22 b	1.35±0.10 ab	159.26±15.46 b	64.49±3.26 a	66.31±1.99 ab

Effects of optimizing fertilization methods on rice yield and soil nutrient balance

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 12

References 21

Related Articles 15

Recommended Articles

Metrics

Comments

处理 Treatment	干重Dry weight
处理 Treatment	叶Leaf	茎Stem	穗Spike
CK	6.03±0.53 d	17.55±1.39 b	44.31±1.12 c
T1	7.43±0.52 a	17.94±0.95 ab	54.61±1.85 a
T2	6.64±0.33 b	17.38±0.69 b	53.23±0.39 a
T3	6.24±0.10 c	18.45±0.46 a	47.69±0.62 b

处理 Treatment	穗数 Spike number/ (10⁴·hm^-2)	穗粒数 Seed number per spike	结实率 Seed setting rate/%	千粒重 1 000-grain weight/g	产量 Yield/(kg·hm^-2)
CK	207.15±4.65 b	190.23±1.66 c	86.62±0.26 a	23.24±0.02 b	9 059.25±240.75 b
T1	265.20±12.45 a	213.63±2.54 a	84.83±1.04 a	23.22±0.07 b	9 397.95±377.55 a
T2	259.65±15.30 a	210.66±3.18 a	81.27±1.55 b	23.21±0.04 b	9 268.65±297.60 a
T3	211.35±6.75 b	195.75±3.11 b	85.85±0.98 a	23.51±0.07 a	8 448.45±289.35 c

处理 Treatment	糙米率 Husked rice rate	整精米率 Head rice yield	直链淀粉含量 Amylose content	精米率 Milled rice rate	蛋白质含量 Protein content
CK	83.1±2.51 a	63.2±2.15 b	17.2±3.21 a	74.4±1.36 a	6.67±0.35 b
T1	83.1±3.25 a	69.8±1.35 a	16.8±1.36 a	74.3±2.14 a	8.04±0.48 a
T2	82.2±2.66 a	71.4±1.11 a	17.1±2.14 a	74.2±2.25 a	7.78±0.55 a
T3	83.0±1.85 a	70.5±1.26 a	17.2±2.16 a	74.1±1.34 a	8.04±0.39 a

[1]	WU Ju, YANG Fei, WU Guoquan, FU Xian, XU Chenguang. Effects of sand culture and soil culture on growth, yield, and quality of cucumber (Cucumis sativus L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1905-1913.
[2]	ZHU Weijing, WU Jia, HONG Chunlai, ZHU Fengxiang, HONG Leidong, ZHANG Tao, ZHANG Shuo, ZHU Huifen. Effects of straw mulching on water, heat, fertility status of soil and yield and quality of flat peach [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1924-1932.
[3]	PEI Huimin, WU Mingming, ZHAI Rongrong, YE Jing, JIN Yue, ZHU Yi, HOU Jianjun, ZHU Guofu, YE Shenghai. Research progress on gene function and breeding of low-cadmium rice cultivars [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 2012-2020.
[4]	ZHANG Zhiying, QIU Qin, HOU Lijuan, XU Ping, JIANG Ning, LIN Jinsheng, LI Huiping, QU Shaoxuan, MA Lin, WANG Weixia, LI Fuhou. Evaluation on the safety of insecticides application on Pleurotus pulmonarius and Auricularia cornea [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1733-1742.
[5]	YAN Fulin, LANG Yunhu, JIAN Yingquan, CHEN Xiongfei, WEI Wei, WANG Zhiwei, AN Jiangyong, REN Deqiang, DING Ning, WEI Shenghua. Response of yield and quality of Radix Ardisia to soil physiochemical properties [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1766-1775.
[6]	TAN Shiyi, YU Guohong, XUE Xianglei, ZHAO Yinglei, XU Baoyu, ZHANG Chenghao. Design and experiment of tray handling device for industrialized rice seedling raising [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1545-1555.
[7]	ZHANG Zhi, HE Haohao, YU Miao, XU Jianfeng. Effects of chemical fertilizer reduction combined with soil conditioner on soil acidity, soil nutrients and rice yield [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1301-1308.
[8]	LIN Xiaobing, LI Jiang, CHENG Yanhong, WANG Binqiang, HE Shaolang, HUANG Shangshu, HUANG Qianru. Effects of organic materials on soil microbial biomass, mineral nitrogen content and rice yield [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1309-1318.
[9]	SU Yang, SHANG Xiaolan, QIAN Zhongming, WU Lingen, HUANG Jiaqi, ZHUANG Haifeng, ZHAO Yufei, DANG Hongyang, XU Lijun. Effects of synergistic enhancement of straw returning to the field with decomposition agent and biochar on soil quality and rice growth [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1139-1148.
[10]	ZHU Xiao, ZHU Ying, LI Hongjun, CHEN Shanfeng. Preparation and quality of oat chickpea compound rice by squeezing [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1149-1158.
[11]	LIU Qihua, SUN Zhaowen, ZHENG Chongke. Effects of nitrogen management on absorption and allocation of microelements in above-ground parts of dry direct-sowing rice [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 987-997.
[12]	DONG Zhichao, YUE Ningyan, LYU Wei, YU Xiaoyi, ZHENG Kaiwen, SONG Haixing, CHEN Haifei. Differential responses of yield, quality, and nitrogen use efficiency to nitrogen application rate in high- and low-oil content rapeseed varieties [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 998-1008.
[13]	LI Yancui, LI Fuqiang, ZHOU Bo. Effects of deficit irrigation at different growth stages on photosynthetic characteristics, yield and quality of Astragalus membranaceus var. mongholicus [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 779-789.
[14]	QIN Yukun, CHEN Junying, WANG Yuping, ZHANG Lijuan. Effects of reducing nitrogen and increasing carbon on cotton production and nitrogen absorption and utilization in the Yangtze River Basin of China [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 869-879.
[15]	YING Yongfei, HAN Dongxuan, MENG Fang, YU Lin, SHEN Jialuan, WANG Kaiying. Effects of biogas slurry substituting chemical fertilizer on rice yield and quality and soil characteristics [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 880-891.

处理 Treatment	氮含量Nitrogen content/(g·kg^-1)			氮肥利用率 Nitrogen use efficiency/%
处理 Treatment	叶Leaf	茎Stem	穗Spike	氮肥利用率 Nitrogen use efficiency/%
CK	7.86±0.38 c	4.75±0.13 b	10.04±0.50 b	—
T1	9.65±0.48 a	8.45±0.71 a	13.70±0.47 a	46.51±1.47 b
T2	9.77±0.33 a	8.67±0.35 a	14.02±0.15 a	50.51±1.21 a
T3	9.05±0.10 b	8.42±0.36 a	14.37±0.52 a	47.37±1.80 b

处理 Treatment	氮含量Nitrogen content/(g·kg^-1)			氮肥利用率 Nitrogen use efficiency/%
处理 Treatment	叶Leaf	茎Stem	穗Spike	氮肥利用率 Nitrogen use efficiency/%
CK	7.86±0.38 c	4.75±0.13 b	10.04±0.50 b	—
T1	9.65±0.48 a	8.45±0.71 a	13.70±0.47 a	46.51±1.47 b
T2	9.77±0.33 a	8.67±0.35 a	14.02±0.15 a	50.51±1.21 a
T3	9.05±0.10 b	8.42±0.36 a	14.37±0.52 a	47.37±1.80 b