Impact of high-temperature stress at grain filling stage on rice grain quality in different rice varieties

doi:10.3969/j.issn.1004-1524.20240196

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (12): 2657-2665.DOI: 10.3969/j.issn.1004-1524.20240196

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Impact of high-temperature stress at grain filling stage on rice grain quality in different rice varieties

TAN Yuhong¹^,²(), ZHOU Min²^,³, ZHANG Hua², ZHANG Heng², WANG Fulin², SONG Tao², ZHU Ying², XU Heng²^,^*()

1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. School of Agriculture, Yunnan University, Kunming 650504, China

Received:2024-02-29 Online:2024-12-25 Published:2024-12-27

Abstract

Abstract:

To investigate the effects of high temperature on the quality of various rice varieties (japonica and indica), three japonica and three indica rice varieties were grown under high temperature (day temperature 35 ℃/night temperature 28 ℃) and control temperature (day temperature 28 ℃/night temperature 22 ℃) during the filling stage. The results revealed that high temperature led to deterioration in rice appearance and reduced seed weight. However, the effect of high temperature on indica rice was significantly less than that on japonica rice. Additionally, the response to high temperature varied among varieties with different haplotype of Wx gene, which controls amylose content in rice seeds. Wx^a-type varieties showed less impact on amylose content under high temperature. In comparison, the Wx^b-type was relatively more affected under high temperature. Although Wx^b-type varieties all exhibited varying degrees of decline in amylose content, the decline was less prominent in indica rice varieties compared with japonica rice varieties under high temperature. This work will help us understand the differences between japonica and indica varieties and Wx allele types in response to high temperatures during the filling stage, which will contribute to the subsequent selection of varieties tolerant to high temperature.

Key words: high temperature, rice, amylose content, variety type

CLC Number:

S511

TAN Yuhong, ZHOU Min, ZHANG Hua, ZHANG Heng, WANG Fulin, SONG Tao, ZHU Ying, XU Heng. Impact of high-temperature stress at grain filling stage on rice grain quality in different rice varieties[J]. Acta Agriculturae Zhejiangensis, 2024, 36(12): 2657-2665.

Figures/Tables 6

Table 1 Primers sequences

引物名称 Primers name	上游引物 Forward primer (5'→3')	下游引物 Reverse primer (5'→3')	产物长度 Product length/bp
Wx(qRT-PCR)	AACGTGGCTGCTCCTTGAA	TTGGCAATAAGCCACACACA	218
BEⅡb(qRT-PCR)	ATGCTAGAGTTTGACCGC	AGTGTGATGGATCCTGCC	261
UBQ10(qRT-PCR)	TGGTCAGTAATCAGCCAGTTTGG	GCACCACAAATACTTGACGAACAG	81
Wx-b(Wx等位基因鉴定Wx allele identification)	CTACAAATAGCCACCCACAC	AAATCTAGGTCAGCTAGCCC	473
WX-mq(Wx等位基因鉴定Wx allele identification)	ACAAACTTCGATCGCTCGTC	GTTCCTTTGAAGTATGGGTTG	734

Fig.1 Effect of filling temperature on the appearance quality of rice RT represents room temperature; HT represents high temperature. The same as below.

Fig.2 Chalkiness of rice in japonica and indica varieties under different temperature treatments RT represents the normal temperature, HT represents the hight temperature. Two-tailed unpaired t-tests were used to determine significant differences (*, P<0.05; **, P<0.01). The same as below.

Fig.3 Comparison of thousand kernel weight of rice in japonica and indica varieties under different temperature treatments

Fig.4 Comparison of rice amylose content between japonica and indica rice varieties under different temperature treatments A, Identification of Wx alleles in different varieties. The red oval represents the difference positions of Wxa and Wxb, the blue oval represents the difference positions of Wxmq. B, Amylose content in rice.

Fig.5 Comparison of expression profiles for starch synthase genes in rice endosperm under different temperature treatments

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Impact of high-temperature stress at grain filling stage on rice grain quality in different rice varieties

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