Present situation and prospects of ecological risk assessment of pesticides on soil organisms

doi:10.3969/j.issn.1004-1524.20250228

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (12): 2642-2648.DOI: 10.3969/j.issn.1004-1524.20250228

• Review • Previous Articles

Present situation and prospects of ecological risk assessment of pesticides on soil organisms

LIAO Jianhua(), GE Feng, SHAN Didi, XU Weili, CAI Xiaoyu, WU Wenzhu^*(), ZHU Yuxuan^*()

Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Ecology and Environment, Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China

Received:2025-03-20 Online:2025-12-25 Published:2026-01-09

Abstract

Abstract:

The application of pesticides has a significant impact on soil organisms, therefore, risk assessment of pesticides to soil organisms is needed in pesticide registration management. In this study, the research progress on ecological risk assessment of pesticides to soil organisms in European Union, United States and China was summarized from the aspects of ecological risk assessment guidelines, ecological effect test standards and exposure assessment models for pesticide registration. Finally, suggestions were put forward for the ecological risk assessment of pesticides to soil organisms, to provide reference for improving the risk assessment system of pesticides to soil organisms in China.

Key words: pesticides, soil organisms, risk assessment

CLC Number:

TQ450.2

LIAO Jianhua, GE Feng, SHAN Didi, XU Weili, CAI Xiaoyu, WU Wenzhu, ZHU Yuxuan. Present situation and prospects of ecological risk assessment of pesticides on soil organisms[J]. Acta Agriculturae Zhejiangensis, 2025, 37(12): 2642-2648.

Figures/Tables 2

References 29

[1]	李湄琳. 农药残留及其对环境的污染[J]. 环境与发展, 2017, 29(9): 56.
	LI M L. Pesticide residues and their environmental pollution[J]. Environment and Development, 2017, 29(9): 56. (in Chinese with English abstract)
[2]	Guidance document on terrestrial ecotoxicology under Council Directive 91/414/EEC[EB/OL]. (2002-10-17) [2025-03-20]. https://food.ec.europa.eu/system/files/2016-10/pesticides_ppp_app-proc_guide_ecotox_terrestrial.pdf.
[3]	Environmental risk assessment scheme for plant protection products[J]. EPPO Bulletin, 2003, 33(2): 195-209.
[4]	European Food Safety Authority. EFSA guidance document for predicting environmental concentrations of active substances of plant protection products and transformation products of these active substances in soil[J]. EFSA Journal, 2017, 15(10): e04982.
[5]	OECD. Guideline 207:earthworm, acute toxicity tests, oecd guidelines for the testing of chemicals[EB/OL]. (1984-04-04) [2025-03-20].https://doi.org/10.1787/9789264070042-en.
[6]	OECD. Guideline 216: soil microorganisms:nitrogen transformation test, oecd guidelines for the testing of chemicals[EB/OL]. (2000-01-21) [2025-03-20]. https://doi.org/10.1787/9789264070226-en.
[7]	OECD. Guideline 217: soil microorganisms:carbon transformation test, oecd guidelines for the testing of chemicals[EB/OL]. (2000-01-21) [2025-03-20]. https://doi.org/10.1787/9789264070240-en.
[8]	OECD. Guideline 220:Enchytraeid reproduction test, OECD guidelines for the testing of chemicals[EB/OL]. (2016-07-29) [2025-03-20].https://doi.org/10.1787/9789264264472-en.
[9]	OECD. Guideline 222:earthworm reproduction test (Eisenia fetida/ Eisenia andrei)[EB/OL]. (2016-07-20) [2025-03-20]. https://doi.org/10.1787/9789264264496-en.
[10]	OECD. Guideline 226:predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil[EB/OL]. (2016-07-29) [2025-03-20].https://doi.org/10.1787/9789264264557-en.
[11]	OECD. Guideline 232:Collembolan reproduction test in soil[EB/OL]. (2016-07-29) [2025-03-20]. https://doi.org/10.1787/9789264264601-en.
[12]	OECD. Guidance document on the breakdown of organic matter in litter bags[EB/OL]. (2006-07-31) [2025-03-20]. https://doi.org/10.1787/a6204552-en.
[13]	BRAAKHEKKE M C, VAN KRAALINGEN D, TIKTAK A, et al. VAN KRAALINGEN D, FOCUSPEARL version 5.5.5: technical description of the databas[EB/OL]. [2025-03-20]. https://edepot.wur.nl/582056.
[14]	CARSEL R F, IMHOFF J C, HUMMEL P R, et al. PRZM-3, a model for predicting pesticide and nitrogen fate in the crop root and unsaturated soil zones:users manual for release 3.0[EB/OL]. [2025-03-20]. https://www.agriculture-xprt.com/articles/przm-3-a-model-for-predicting-pesticide-and-nitrogen-fate-in-the-crop-root-and-unsaturated-soil-zone-5791#:-:text=PRZM-3%20is%20the%20most%20recent%20version%20of%20a,through%20the%20crop%20root%20and%20unsaturated%20soil%20zones.
[15]	European Food Safety Authority. EFSA guidance document for predicting environmental concentrations of active substances of plant protection products and transformation products of these active substances in soil[J]. EFSA Journal, 2015, 13(4): 4093.
[16]	US Environmental Protection Agency. Guidelines for ecological risk assessment[EB/OL]. (1998-05-14) [2025-03-20].https://www.epa.gov/sites/default/files/2014-11/documents/eco_risk_assessment1998.pdf.
[17]	US Environmental Protection Agency. Guidance for developing ecological soil screening levels[EB/OL]. [2025-03-20].https://www.epa.gov/sites/default/files/2015-09/documents/ecossl_guidance_chapters.pdf.
[18]	US Environmental Protection Agency. Ecological effects test guidelines OSCPP 850. 3100: earthworm subchronic toxicity test[EB/OL]. [2025-03-20]. https://nepis.epa.gov/Exe/ZyNET.exe/P100IREJ.TXTZyActionD=ZyDocument&Client=EPA&Index=2011+Thru+2015&Docs=&Query=&Time=&EndTime=&SearchMethod=1&TocRestrict=n&Toc=&TocEntry=&QField=&QFieldYear=&QFieldMonth=&QFieldDay=&IntQFieldOp=0&ExtQFieldOp=0&XmlQuery=&File=D%3A%5Czyfiles%5CIndex%20Data%5C11thru15%5CTxt%5C00000010%5CP100IREJ.txt&User=ANONYMOUS&Password=anonymous&SortMethod=h%7C-&MaximumDocuments=1&FuzzyDegree=0&ImageQuality=r75g8/r75g8/x150y150g16/i425&Display=hpfr&DefSeekPage=x&SearchBack=ZyActionL&Back=ZyActionS&BackDesc=Results%20page&MaximumPages=1&ZyEntry=1&SeekPage=x&ZyPURL.
[19]	US Environmental Protection Agency. Ecological effects test guidelines OSCPP 850. 3200: soil microbial community toxicity test[EB/OL]. [2025-03-20].https://nepis.epa.gov/Exe/ZyNET.exe/P100IRE6.TXT ZyActionD=ZyDocument&Client=EPA&Index=2011+Thru+2015&Docs=&Query=&Time=&EndTime=&SearchMethod=1&TocRestrict=n&Toc=&TocEntry=&QField=&QFieldYear=&QFieldMonth=&QFieldDay=&IntQFieldOp=0&ExtQFieldOp=0&XmlQuery=&File=D%3A%5Czyfiles%5CIndex%20Data%5C11thru15%5CTxt%5C00000010%5CP100IRE6.txt&User=ANONYMOUS&Password=anonymous&SortMethod=h%7C-&MaximumDocuments=1&FuzzyDegree=0&ImageQuality=r75g8/r75g8/x150y150g16/i425&Display=hpfr&DefSeekPage=x&SearchBack=ZyActionL&Back=ZyActionS&BackDesc=Results%20page&MaximumPages=1&ZyEntry=1&SeekPage=x&ZyPURL.
[20]	程燕, 周军英, 单正军. 美国农药水生生态风险评价研究进展[J]. 农药学学报, 2005, 7(4): 293-298.
	CHENG Y, ZHOU J Y, SHAN Z J. Progress of study on aquatic ecological risk assessment of pesticides in USA[J]. Chinese Journal of Pesticide Science, 2005, 7(4): 293-298. (in Chinese with English abstract)
[21]	ISO. Soil quality: effects of pollutants on earthworms: part 3: guidance on the determination of effects in field situations[S/OL]. (2014-10-15) [2025-03-20].https://cdn.standards.iteh.ai/samples/57583/671403bca62b4f22860fc3ec4f5d7bdf/ISO-11268-3-2014.pdf
[22]	文伯健, 李文娟. China-PEARL和PRZM-GW模型潍坊市场景农药地下水风险评估研究[J]. 农业资源与环境学报, 2014, 31(5): 401-410.
	WEN B J, LI W J. Pesticide groundwater risk assessment with China-PEARL and PRZM-GW model in Weifang City scenario[J]. Journal of Agricultural Resources and Environment, 2014, 31(5): 401-410. (in Chinese with English abstract)
[23]	刘寒冰, 王冬琦, 薛南冬, 等. 土壤中毒死蜱及主要代谢产物的降解与生态风险[J]. 农业环境科学学报, 2020, 39(10): 2446-2454.
	LIU H B, WANG D Q, XUE N D, et al. Degradation characteristics and ecological risk of chlorpyrifos and results of its degradation in dryland soils[J]. Journal of Agro-Environment Science, 2020, 39(10): 2446-2454. (in Chinese with English abstract)
[24]	周艳明, 袁善奎, 单炜力. 农药代谢物的生态风险评估方法[J]. 农药科学与管理, 2023, 44(11): 23-29, 44.
	ZHOU Y M, YUAN S K, SHAN W L. Ecological risk assessment method for pesticide metabolite[J]. Pesticide Science and Administration, 2023, 44(11): 23-29, 44. (in Chinese with English abstract)
[25]	刘鑫, 王蕾, 胡飞龙, 等. 《生物多样性公约》下有关农药化肥减量化要求及我国的对策建议[J]. 生态与农村环境学报, 2021, 37(9): 1129-1136.
	LIU X, WANG L, HU F L, et al. Requirements for reduction of pesticides and fertilizers under the convention on biological diversity and the countermeasure suggestions for China[J]. Journal of Ecology and Rural Environment, 2021, 37(9): 1129-1136. (in Chinese with English abstract)
[26]	张天竞, 周倩, 杨海荣. 白符跳虫在农药环境风险评估中的应用[C]// 中国毒理学会毒理学替代法与转化毒理学专业委员会, 中国环境诱变剂学会毒性测试与替代方法专业委员会, 中国毒理学会食品毒理学专业委员会. 2021(第五届)毒性测试替代方法与转化毒理学(国际)学术研讨会会议论文集. 沈阳: 沈阳沈化院测试技术有限公司, 2021: 1.
[27]	王蕾, 姜锦林, 董姝楠, 等. 我国土壤环境基准受试无脊椎动物筛选研究[J]. 生态毒理学报, 2024, 19(1): 91-102.
	WANG L, JIANG J L, DONG S N, et al. Screening of test invertebrate organisms for deriving soil environmental criteria in China[J]. Asian Journal of Ecotoxicology, 2024, 19(1): 91-102. (in Chinese with English abstract)
[28]	唐跃明, 田大军, 章超, 等. 纳米农药研究进展[J]. 世界农药, 2024, 46(3): 20-25.
	TANG Y M, TIAN D J, ZHANG C, et al. Research progress of nano-pesticides[J]. World Pesticide, 2024, 46(3): 20-25. (in Chinese with English abstract)
[29]	张文博, 潘兴鲁, 吴小虎, 等. 纳米农药风险评价研究进展[J]. 现代农药, 2023, 22(2): 36-39.
	ZHANG W B, PAN X L, WU X H, et al. Research advance of nanopesticide risk assessment[J]. Modern Agrochemicals, 2023, 22(2): 36-39. (in Chinese with English abstract)

测试准则Test guidelines	测试号Test No.
蚯蚓急性毒性试验Earthworm, acute toxicity tests	OECD 207-1984^[5]
土壤微生物毒性试验(氮转化方法)Soil microorganisms nitrogen transformation test	OECD 216-2000^[6]
土壤微生物毒性试验(碳转化方法)Soil microorganisms carbon transformation test	OECD 217-2000^[7]
线蚓生殖测试Enchytraeid reproduction test	OECD 220-2016^[8]
蚯蚓繁殖毒性试验Earthworm reproduction test(Eisenia fetida/Eisenia andrei)	OECD 222-2016^[9]
土壤中捕食性螨虫繁殖试验Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil	OECD 226-2016^[10]
土壤中跳虫繁殖测试Collembolan reproduction test in soil	OECD 232-2016^[11]
有机质分解袋试验Guidance document on the breakdown of organic matter in litter bags	OECD 56-2006^[12]

测试准则Test guidelines	测试号Test No.
蚯蚓急性毒性试验Earthworm, acute toxicity tests	OECD 207-1984^[5]
土壤微生物毒性试验(氮转化方法)Soil microorganisms nitrogen transformation test	OECD 216-2000^[6]
土壤微生物毒性试验(碳转化方法)Soil microorganisms carbon transformation test	OECD 217-2000^[7]
线蚓生殖测试Enchytraeid reproduction test	OECD 220-2016^[8]
蚯蚓繁殖毒性试验Earthworm reproduction test(Eisenia fetida/Eisenia andrei)	OECD 222-2016^[9]
土壤中捕食性螨虫繁殖试验Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil	OECD 226-2016^[10]
土壤中跳虫繁殖测试Collembolan reproduction test in soil	OECD 232-2016^[11]
有机质分解袋试验Guidance document on the breakdown of organic matter in litter bags	OECD 56-2006^[12]

Present situation and prospects of ecological risk assessment of pesticides on soil organisms

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