Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

›› 2010, Vol. 22 ›› Issue (6): 716-721.

• 论文 • Previous Articles Next Articles

Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

ZHANG Yong;ZHANG Zhen;QIU Hai-ping;WANG Yan-li;WANG Jiao-yu;SUN Guo-chang;*

¹Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou 310012, China;²Institue of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-11-25 Published:2010-11-25

Abstract

Abstract: The gene fragments of Gα subunits were isolated from Colletotrichum hanaui by using homologue cloning method. The 5′and 3′ flanking sequences of the fragments were cloned by TAIL-PCR method and sequenced. After sequence assembling, the complete senquences of cagA, cagB and cagC were obtained. The structures of the three genes were characterized by comparing genome and CDS sequences. The cagA has an open reading frame (ORF) of 1 380 bp interrupted by 5 introns and putatively encodes a 355aa protein. The cagB has an ORF of 1 283 bp interrupted by 3 introns and putatively encodes a 353aa protein. The cagC has an ORF of 1382 bp interrupted by 4 introns and putatively encodes a 354aa protein. The results of amino acid sequence alignments showed that the predicated proteins were homologous with Gα subunits of other ascomycetous phytopathogens such as Magnaporthe grisea, Gibberella zeae, with the amino acid identity between 75% and 99%, respectively.

Key words: Colletotrichum hanaui, G protein, Gα subunit, gene clone, sequence analysis

ZHANG Yong;ZHANG Zhen;QIU Hai-ping;WANG Yan-li;WANG Jiao-yu;SUN Guo-chang;*. Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui[J]. , 2010, 22(6): 716-721.

[1]	WANG Wenqi, WANG Panpan, ZHANG Yanling, LIU Qingqing, HONG Shuangshuang, ZHAO Gaopeng, LIU Hongchang, WANG Cuiling. Screening of proteins interacting with circadian clock gene ZmPRR1-2 in maize [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 977-986.
[2]	YU Huan, LI Hui, CHEN Youbo, SHI Yushi, ZHAO Depeng, LONG Xia, TAN Qisong. Screening and functional analysis of proteins interacting with chicken adenylosuccinate lyase [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 515-526.
[3]	LUO Hailin, YUAN Lei, YAN Jiahui, GUO Qingyun. Cloning and identification of Pepper cryptic virus 2 in Qinghai Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1368-1374.
[4]	YAO Yanlin, MA Li, LIU Lijun, PU Yuanyuan, LI Xuecai, WANG Wangtian, FANG Yan, SUN Wancang, WU Junyan. Bioinformatics and expression analysis of flowering regulation gene BrFT in Brassica rapa L. [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 992-1000.
[5]	LIU Junwen, WANG Di, ZHU Yanyan, XING Gang, ZHAN Songhe, LIU Xiaolu, WEI Jianzhong, SUN Pei, LIU Xuelan, LI Yu. Establishment and application of an indirect ELISA based on protein by expression and cloning of CbpB gene of Erysipelothrix rhusiopathiae [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 816-824.
[6]	HE Jiaqi, ZHAI Ying, ZHANG Jun, QIU Shuang, LI Mingyang, ZHAO Yan, ZHANG Meijuan, MA Tianyi. Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 1-7.
[7]	LIU Kai, FENG Xiaoyu, MA Hengjia, XIE Nan. Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River [J]. , 2020, 32(9): 1591-1608.
[8]	NI Zheng, CHEN Liu, HUA Jionggang, YE Weicheng, YUN Tao, ZHU Yinchu, ZHANG Cun. Detection of leukaemia virus antigen and analysis of GP85 gene sequence in local chicken breeds in Zhejiang Province [J]. , 2020, 32(8): 1357-1362.
[9]	WANG Weike, SONG Jiling, LU Na, YUAN Weidong, YAN Jing, CHEN Guanping. Cloning and expression of the PpFBD1 involved in primordium formation of Pleurotus pulmonarius [J]. , 2020, 32(1): 93-97.
[10]	DEXI Cuomu, WANG Yin, YANG Zexiao, YAO Xueping, LUO Yan, LIAO Changyu, ZHANG Pengfei, JIANG Dike, XIANG Mingyuan, JIANG Ruijiao, SONG Yong. Epidemiological investigation of porcine epidemic diarrhea virus and sequence analysis of ORF3 and S1 genes in Sichuan from 2016 to 2018 [J]. , 2019, 31(9): 1423-1428.
[11]	YI Keke, YIN Wenqi, ZHOU Yuancheng, JIANG Jinzhen, ZHANG Baiyu, LI Zhongyin, YAN Qigui. Isolation and identification of 4 strains of porcine pseudorabies virus and analysis of main virulence genes [J]. , 2019, 31(9): 1429-1436.
[12]	LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila [J]. , 2019, 31(9): 1461-1470.
[13]	WANG Yuanhong, LU Zhimin, ZHANG Xueqi, LIU Zimin, HU Zihui, YANG Kankan, LIU Hongmei, PAN Ling, PENG Kaisong, LI Yongdong, WANG Yong. Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus [J]. , 2019, 31(5): 709-715.
[14]	ZHU Yu, LIU Yang. Bioinformatics analysis of odorant-binding proteins and chemosensory proteins of Pieris rapae [J]. , 2019, 31(1): 104-112.
[15]	BA Shaobo, SHI Lin, LI Qunjing, LIU Zhengkui, CHEN Lin, WANG Xiaodu, SONG Houhui. Isolation, identification and molecullar epidemiological analysis of 17-ZJ-HZ strain of porcine reproductive and respiratory syndrome virus [J]. , 2018, 30(8): 1303-1311.

Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments