Abstract: House keeping gene GAPDH(glyceraldehyde-3-phosphate dehydrogenase) was often used as inner control in quantitative or semi-quantitative PCR test to determine relative expressive amount of target genes. Based on EST monoclones of GAPDH gene obtained from Guzmania full length cDNA library, its full length cDNA sequence was obtained by Primer Walking sequencing. The gene, designated GoGAPDH1, was 1 790 bp in length encoding 421 amino acids. Some characters involving in cDNA sequence, amino acid sequence, conservative domain and genetic relationship were analyzed by Blast, ExPASy, ProtParam, SPOMA, Find Conserved Domains(NCBI), ClustalX and MEGA softwares． The results showed that GoGAPDH1 shared 82% similarity with cytoplasmic GAPDH in maize, which indicated that GoGAPDH1 might be a cytoplasmic GAPDH.

Key words: Guzmania(Guzmania Ruiz&, Pav), glyceraldehyde-3-phosphate dehydrogenase, clone