Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

›› 2010, Vol. 22 ›› Issue (6): 716-721.

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Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

ZHANG Yong;ZHANG Zhen;QIU Hai-ping;WANG Yan-li;WANG Jiao-yu;SUN Guo-chang;*

¹Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou 310012, China;²Institue of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-11-25 Published:2010-11-25

Abstract

Abstract: The gene fragments of Gα subunits were isolated from Colletotrichum hanaui by using homologue cloning method. The 5′and 3′ flanking sequences of the fragments were cloned by TAIL-PCR method and sequenced. After sequence assembling, the complete senquences of cagA, cagB and cagC were obtained. The structures of the three genes were characterized by comparing genome and CDS sequences. The cagA has an open reading frame (ORF) of 1 380 bp interrupted by 5 introns and putatively encodes a 355aa protein. The cagB has an ORF of 1 283 bp interrupted by 3 introns and putatively encodes a 353aa protein. The cagC has an ORF of 1382 bp interrupted by 4 introns and putatively encodes a 354aa protein. The results of amino acid sequence alignments showed that the predicated proteins were homologous with Gα subunits of other ascomycetous phytopathogens such as Magnaporthe grisea, Gibberella zeae, with the amino acid identity between 75% and 99%, respectively.

Key words: Colletotrichum hanaui, G protein, Gα subunit, gene clone, sequence analysis

ZHANG Yong;ZHANG Zhen;QIU Hai-ping;WANG Yan-li;WANG Jiao-yu;SUN Guo-chang;*. Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui[J]. , 2010, 22(6): 716-721.

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Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui

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