Abstract: The present study aimed at selecting the stable reference genes to ensure the reliability and accuracy of gene expression analysis in kiwifruit for qRT-PCR. The expressions of four frequently-used reference genes （ACTB，TUB，18S rRNA，GAPDH） were analyzed in different tissues and fruits at different developmental stages， respectively. According to the analysis by GeNorm， the stabilities of the four candidate reference genes were different， TUB and ACTB were both stably expressed in six different tissues， which were recommended for genes expression normalization in different kiwifruit tissues. 18S rRNA was the most stable gene in different developmental stages of White kiwifruit, 18S rRNA and TUB were most suitable for normalization of mRNA expression levels in White kiwifruit.

Key words: kiwifruit, reference genes, qRT-PCR