Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

doi:10.3969/j.issn.1004-1524.2016.12.04

›› 2016, Vol. 28 ›› Issue (12): 1985-1991.DOI: 10.3969/j.issn.1004-1524.2016.12.04

• Animal Science • Previous Articles Next Articles

Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

ZHANG Tao, LIU He-he^*, LUO Jun, LI Liang, HUANG Hui-lan, HE Hua

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-06-03 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: To study the effect of single nucleotide polymorphism (SNP) of duck IL-2 gene regulatory region on transcriptional regulation, a 2 850 bp promoter sequence of duck IL-2 gene was obtained by cloning. The homology analysis showed that the homology of duck IL-2 promoter sequence with human, mouse and chicken was 35.37%, 37.52% and 34.74%, respectively. The core transcription factor binding sites was concentrated at the region (-1400/-1000). The detecting of SNP and genetic polymorphism of duck IL-2 gene promoter fragment (-1932/-742) found two polymorphism sites (C-1406T and C-1353A). Allele A was the predominant allele, and both polymorphism sites were not in Hardy-Weinberg. Correlation analysis showed that the mRNA expression levels of IL-2 gene and IL-2 protein levels had no significant relationship with different genotypes of the polymorphism sites. However, the expression levels of IL-2 mRNA in individuals with genotype AA were higher than those in other genotypes (P>0.05), suggesting that the allele A might promote the transcription of IL-2 gene. The results provided a theoretical basis for studies on the transcription regulation mechanism of IL-2 gene.

Key words: duck, IL-2, single nucleotide polymorphism, transcriptional regulation region, transcription level

CLC Number:

S834
Q754

ZHANG Tao, LIU He-he, LUO Jun, LI Liang, HUANG Hui-lan, HE Hua. Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels[J]. , 2016, 28(12): 1985-1991.

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Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

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