Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes

doi:10.3969/j.issn.1004-1524.2017.12.06

›› 2017, Vol. 29 ›› Issue (12): 1994-1999.DOI: 10.3969/j.issn.1004-1524.2017.12.06

• Animal Science • Previous Articles Next Articles

Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes

HUANG Shulun¹, ZHENG Kaiwen¹, HU Yang¹, LI Zhangcheng¹, CHENG Fangjun^{1, 2, *}, SONG Zhenhui^{1, 2}, ZHOU Zuoyong^{1, 2}, LIU Guolin¹, LIN Chunfa¹, LIN Yongrun¹, ZHANG Tingting¹

1.College of Animal Science, Southwest University, Chongqing 402460, China;
2. Veterinary Science Engineering Research Center of Chongqing, Chongqing 402460, China

Received:2017-05-15 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: To study the generation and molecular characteristics of Escherichia coli typeⅠfimbriae from ducks in parts of Sichuan-Chongqing region, the typeⅠfimbriae was identified by MSHA/MRHA test, and their FimA and FimH genes were identified by PCR amplification; The hydrophilicity, antigenicity, and surface probability of FimA amino acid sequences were analyzed by DNAStar to predict antigenic sites. The MSHA/MRHA test results showed that the positive rate of MSHA was 44.3%. PCR results indicated that the positive rate of FimA and FimH were 63.41% and 100%, respectively. Amino acid sequences of FimA and FimH were deduced and contrasted respectively with comparative Escherichia coli strain ECOR61 and ECOR45. Amino acid sequence alignment showed that there were 19 different positions in FimA amino acid sequences. However, there were 1-2 different positions in FimH amino acid sequences, it was believed that FimH amino acid sequences were highly conserved. The antigenic sites of FimA amino acid sequences were predicted. The results indicated that antigenic epitopes of most strains were distributed in 84-88, 142-146 and 149-150 amino acids. In addition, some other strains' antigenic epitopes appeared at 101-105 and 130-134 amino acids.

Key words: Escherichia coli, typeⅠ, fimbriae, amino acid, sequence alignment, antigenic epitope

CLC Number:

S855.3

HUANG Shulun, ZHENG Kaiwen, HU Yang, LI Zhangcheng, CHENG Fangjun, SONG Zhenhui, ZHOU Zuoyong, LIU Guolin, LIN Chunfa, LIN Yongrun, ZHANG Tingting. Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes[J]. , 2017, 29(12): 1994-1999.

References

[1] 马兴树. 禽大肠杆菌病疫苗研究进展[J]. 中国畜牧兽医, 2015, 42(1): 234-244.
MA X S. Research progress on vaccines of avian colibacillosis[J]. China Animal Husbandry & Veterinary Medicine , 2015, 42(1): 234-244. (in Chinese with English abstract)
[2] TIVENDALE K A, LOGUE C M, KARIYAWASAM S, et al. Avian pathogenic Escherichia coli are similar to neonatal meningitis E.coli strains and are able to cause meningitis in the rat model of human disease[J]. Infection and Immunity , 2010, 78 (8): 3412-3419.
[3] DZIVA F, STEVENS M P. Colibacillosis in poultry: unraveling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts[J]. Avian Pathology , 2008, 37(4): 355-366.
[4] DOZOIS CM, CHANTELOUP N, DHO-MOULIN M, et al. Bacterial colonization and in vivo expression of F1 (type 1) fimbrial antigens in chickens experimentally infected with pathogenic Escherichia coli [J]. Avian diseases , 1994,38(2): 231-239.
[5] KROGFELT K A, BERGMANS H, KLEMM P. Direct evidence that the FimH protein is the mannose-specific of Escherichia coli type 1 fimbriae[J]. Infection and Immunity , 1990, 58(6): 1995-1998.
[6] HERNANDES R T, VELSKO I, SAMPAIO S C, et al. Fimbrial adhesins produced by atypical enteropathogenic Escherichia coli strains[J]. Applied and Environmental Microbiology , 2011, 77(23): 8391-8399.
[7] CHAPMAN T A, WU X Y, BARCHIA I, et al. Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine[J]. Applied and Environmental Microbiology , 2006, 72(7): 4782-4795.
[8] JOHNSON J R, SWANSON J L, BARELA T J, et al. Receptor specificities of variant Gal(alphal-4) Gal-binding PapG adhesins of uropathogenic Escherichia coli as assessed by hemagglutination phenotypes[J]. The Journal of Infectious Diseases , 1997, 175(2): 373-381.
[9] MALAVIYA R, IKEDA T, ROSS E, et al. Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha[J]. Nature , 1996, 381: 77-80.
[10] MUNDI H, BJÖRKSTÉN B, SVANBORG C, et al. Extracellular release of reactive oxygen species from human neutrophils upon interaction with Escherichia coli strains causing renal scarring[J]. Infection and Immunity , 1991, 59(11): 4168-4172.
[11] STEADMAN R, TOPLEY N, JENNER D E, et al. Type 1 fimbriate Escherichia coli stimulates a unique pattern of degranulation by human polymorphonuclear leukocytes[J]. Infection and Immunity , 1988, 56(4): 815-822.
[12] 于小娜. 鸭源致病性大肠杆菌Ⅰ型菌毛 pilA 基因序列分析与原核表达产物免疫原性研究[D]. 雅安: 四川农业大学, 2006.
YU X N. The sequence analysis and research in immunogenicity of the prokaryotic expression product of pilA gene of pathogenic E.coli isolated from ducks[D]. Ya'an: Sichuan Agriculture University, 2006. (in Chinese with English abstract)
[13] 叶如俊. 鸡源致病性大肠杆菌1型菌毛 pilA 基因的PCR克隆及序列分析[D]. 雅安: 四川农业大学, 2002.
YE R J. The PCR and sequence analyse of pilA gene of type 1 pili of pathogenic Escherichia coli isolated from chick[D]. Ya'an: Sichuan Agriculture University, 2002. (in Chinese with English abstract)
[14] 王剑飞. fimH 基因对尿路致病性大肠埃希菌1型菌毛粘附功能影响的初步探索[D]. 天津: 天津医科大学, 2014.
WANG J F. Primary exploration on the role of fimH gene in uropathogenic Escherichia coli Type 1 fimbriae adhesion[D]. Tianjin: Tianjin Medical University, 2014. (in Chinese with English abstract)
[15] WITHMAN B, GUNASEKERA T S, BEESETTY P, et al. Transcriptional responses of uropathogenic Escherichia coli to increased environmental osmolality caused by salt or urea[J]. Infection and Immunity , 2013, 81(1): 80-89.
[16] 叶如俊, 王红宁, 谭丙乾.鸡源致病性大肠杆菌1型菌毛 pilA 基因的PCR扩增克隆及序列分析[J]. 畜牧兽医学报, 2004, 35(1): 83-88.
YE R J, WANG H N, TAN B Q. The PCR and sequence analyse of pilA gene of type 1 pili of pathogenic Escherichia Coli isolated from chick[J]. Acta Veterinaria et Zootechnica Sinica , 2004, 35(1): 83-88. (in Chinese with English abstract)

Identification of type Ⅰ fimbriae of Escherichia coli isolated from ducks and genetic characteristics of their FimA and FimH genes

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Guangmin, LIU Xiuting, DAI Bing, YANG Hua, LYU Wentao, WANG Yuanxia, XIAO Yingping. Effects of early weaning and dietary glutamine addition on serum free amino acids and their metabolites in piglets [J]. , 2020, 32(10): 1764-1771.
[2]	YANG Hua, MA Yan, LIU Xiuting, LYU Wentao, LU Lizhi, XIAO Yingping. Virulence genes and drug resistance characteristics of Escherichia coli in ready-to-eat duck products [J]. , 2020, 32(10): 1841-1848.
[3]	MA Jie, ZHENG Hao, ZHOU Ping, CHEN Chunyan, WU Rui, MA Wei, SONG Lei, SUN Bo. Cloning and sequence analysis of StBSKs gene of Solanum tuberosum [J]. , 2019, 31(8): 1224-1230.
[4]	CHANG Jiang, LUO Yi, TANG Biao, ZHANG Ling, DAI Xianjun, QIU Hanqi, YANG Hua, XIA Xiaodong. Whole-genome sequencing and antibiotic resistance study of a multi-drug resistant Escherichia coli isolated from chicken [J]. , 2019, 31(8): 1249-1256.
[5]	LIU Yongtao, DONG Jing, XIA Jingjin, CAO Cuiyu, XU Ning, YANG Qiuhong, AI Xiaohui. Effect and evaluation of different feed on texture properties and nutritional quality of Procambarus clarkia cultured in rice fields [J]. , 2019, 31(12): 1996-2004.
[6]	PENG Kenan, ZHOU Xueke, YIN Xinhuan, LI fei, CAI Yao, ZENG Yubing, JIANG Chaoyuan, ZHANG Rubo, YANG Zexiao, XU Zhiwen, ZHU Ling. Molecular typing, biofilm formation ability and drug resistance of pathogenic Escherichia coli isolated from pigs in Sichuan Province [J]. , 2019, 31(10): 1599-1607.
[7]	LIU Cuiyan, CHEN Siyu, SUN Taotao, SHANG Lili, YANG Mingchuan, YANG Zisheng, HAN Chunyang. Preventive effect of purslane combined with probiotics on heat-toxin syndrome diarrhea mice [J]. , 2018, 30(2): 211-219.
[8]	YANG Hua, HE Xiangxiang, XIAO Yingping, QIAN Mingrong, ZHANG Qiaoyan, TANG Biao. Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province [J]. , 2017, 29(6): 888-893.
[9]	YE Riying, WANG Yaling, HOU Hongwei, ZHANG Chunhui, SUN Lijun, XU Defeng. Comparison and analysis of nucleoside, cordycepic acid and amino acid content in insect body and stroma of Cordyceps gryllotalpae [J]. , 2017, 29(5): 824-830.
[10]	MA Linlong, JIN Xiaofang, CAO Dan, LIU Yanli, GONG Ziming, WANG Shengpeng, LIU Panpan. Suitability for processing green tea from high amino acid content of tea introduced to Hubei Province [J]. , 2017, 29(2): 251-260.
[11]	WEI Shuya, ZHAO Xudong, WANG Huiping, YANG Guangxiao, HE Guangyuan. Cloning and encoding protein bioinformatics analysis of BdCDPK4 from Brachypodium distachyon [J]. , 2017, 29(2): 345-352.
[12]	LIU Yong-liang, CHEN Bo, LIU Ming-gang, XU Yu-qin, DAI Xi-xi, GU Xiao-xiao, PAN Kang-cheng. Effect of Lianmei dysentery Chinese herbal preparation on weaned piglet diarrhea by an Escherichia coli (K88) experimental infection [J]. , 2016, 28(9): 1493-1500.
[13]	ZHANG Qiang1，2WU Caie1，*. Physicochemical properties and antioxidant activities of protein from Morchella esculenta mycelium [J]. , 2016, 28(8): 1408-.
[14]	CHEN Xiu\\|ping， XIAO Zheng\\|run， LIN Jing， WANG Yan\\|qiu， ZHANG Jie， HUANG Qi\\|fa， YU Dao\\|jin*. Effect of nitrogen and phosphorous on tetracycline resistance of Escherichia coli [J]. , 2016, 28(2): 247-.
[15]	LIU Yuan\\|jing1，2， ZHOU Yu2， CHEN Wen\\|xue1， WANG Wen2， WANG Qiang1，2，*. Study on the antimicrobial cleansing methods for reducing microorganism in strawberry#br# [J]. , 2015, 27(8): 1422-.