HPLC fingerprint of peanut hulls from different habitats by cluster analysis

doi:10.3969/j.issn.1004-1524.2018.12.20

›› 2018, Vol. 30 ›› Issue (12): 2137-2143.DOI: 10.3969/j.issn.1004-1524.2018.12.20

• Food Science • Previous Articles Next Articles

HPLC fingerprint of peanut hulls from different habitats by cluster analysis

ZHAO Liang, CHEN Yue, FANG Yingguo, RAO Guiwei^*

College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China

Received:2018-03-09 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: The HPLC fingerprints of peanut shell samples from 8 habitats were studied and cluster analysis was carried out. The conditions of sample extraction and chromatographic were optimized. The optimized chromatographic conditions were WondaCract ODS-2 C₁₈ (4.6 mm×150 mm, 5 μm) column and gradient elution with acetonitrile-0.5% phosphate solution as mobile phase. The flow rate was 1 mL·min^-1, the column temperature was 35 ℃, and the wavelength was 290 nm. The HPLC fingerprints of peanut shell samples from 8 regions were established, indicating 14 stable common peaks. The similarity of common patterns was larger than 0.9 except Xinchang Xiaojingsheng. The cluster analysis of the average peak area of the common peaks showed that the 8 peanut shells could be divided into two categories.

Key words: peanut shell, HPLC fingerprint, cluster analysis

CLC Number:

ZHAO Liang, CHEN Yue, FANG Yingguo, RAO Guiwei. HPLC fingerprint of peanut hulls from different habitats by cluster analysis[J]. , 2018, 30(12): 2137-2143.

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HPLC fingerprint of peanut hulls from different habitats by cluster analysis

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