Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

doi:10.3969/j.issn.1004-1524.2018.04.02

›› 2018, Vol. 30 ›› Issue (4): 528-536.DOI: 10.3969/j.issn.1004-1524.2018.04.02

• Plant Protection • Previous Articles Next Articles

Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

WU Degong, FANG Wenhao, DU Junli^*, YU Haibing, WANG Changjin, LI Jinpeng, CHU Lina, TANG Li

College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China

Received:2017-08-01 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: Two different aphid resistant maize (Zea mays L.) varieties, Zhengdan 958(resistant variety) and Liyu 16(susceptible variety),were used in this study, and the dynamic changes of malondialdehyde (MDA), soluble sugar, chlorophyll (Chl), soluble protein and five protective enzymes were measured after different density of aphids (0,10,20,40,80 heads per plant) suction stress in three leaf stages. The results showed that: in the different density of aphids sucking stress, the soluble sugar content of Zhengdan 958 and Liyu 16 increased with the density of aphids, and the growth rate of Zhengdan 958 was less than Liyu 16. Chlorophyll content of two varieties decreased with aphid density increased, and the decrease rate of Liyu 16 was higher than that of Zhengdan 958. The change of soluble protein content was almost the same as the content of malondialdehyde (MDA), showing an upward trend, but the percentage change of MDA, soluble protein content in Zhengdan 958 was lower than that of Liyu 16.Therefore, there was a negative correlation between chlorophyll content and aphid density, while soluble sugar content, soluble protein and malondialdehyde (MDA) content were positively correlated with aphid density. The chlorophyll content change rate could be used as an index of aphid resistance, but the soluble sugar content, soluble protein and malondialdehyde (MDA) content were negatively correlated with the aphid resistance of maize. The activities of superoxide dismutase(SOD), peroxidase(PPO) and catalase(CAT) in Zhengdan 958 and Liyu 16 increased first and then decreased with the increase of aphid density. But the activities of five protective enzymes were higher than the control group; while the activity of polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL) showed upward trend with the increase of aphid density. According to the results, it can be seen that the enzyme activity was related to the density of aphid at maize seedling stage. PPO and PAL play a leading role in the protective enzymes of maize, and SOD, POD and CAT play a protective role in lower aphid density.

Key words: Rhopalosiphum maidis, Zea mays, MDA, soluble sugar, chlorophyll, soluble protein, protective enzyme

CLC Number:

S433.1

WU Degong, FANG Wenhao, DU Junli, YU Haibing, WANG Changjin, LI Jinpeng, CHU Lina, TANG Li. Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage[J]. , 2018, 30(4): 528-536.

References

[1] 郭春爱.中国玉米消费分析与展望[J]. 农业展望,2016 (8):1-5.
GUO C A. Analysis and prospect of maize consumption in China[J]. Agriculture Outlook , 2016 (8):1-5. (in Chinese)
[2] 杨俊芸,陈洪梅,谭静.大力发展青贮玉米促进云南畜牧业发展:云南省发展青贮玉米的思考[J].西南农业学报,2014,17(增刊):325-329.
YANG J Y, CHEN H M, TAN J. Promoting silage maize for advancing stock breeding in Yunnan province[J]. Southwest China Journal of Agricultural Sciences , 2014,17(S):325-329. (in Chinese with English abstract)
[3] BLACKMAN R L, EASTOP V F. Aphids on the world's crops: An identification and information guide[M]. New York: John Wiley & Sons, 2000:466.
[4] 武德功,杜军利,王森山,等. 4个苜蓿品种对豌豆蚜的抗性评价[J]. 草业科学,2012,29(1):101-104.
WU D G, DU J L, WANG S S, et al. Evaluation on resistance of 4 alfalfa ( Medicago sativa ) cultivars to pea aphid ( Acyrthosiphon pisum )[J]. Pratacultural Science , 2012, 29(1): 101-104. (in Chinese with English abstract)
[5] 李远,赵曼,郭线茹,等. 不同玉米品种(系)田间抗蚜性的初步鉴定[J]. 河南农业大学学报,2012,46(3):307-312.
LI Y, ZHAO M, GUO X R, et al. Field identification of resistance in different maize hybrids and inbredlines to Rhopalosiphum maidis Fitch[J]. Journal of Henan Agricultural University , 2012, 46(3):307-312. (in Chinese with English abstract)
[6] 徐雪,吕晓坤,何力, 等. 玉米不同品种对玉米蚜的抗性鉴定[J]. 云南农业大学学报(自然科学版),2013,28(4):598-601.
XU X, LYU X K, HE L, et al. Study on resistance of different corn varieties to the aphid( Rhopalosiphum maidis Fitch)[J]. Journal of Yunnan Agricultural University , 2013, 28(4): 598-601. (in Chinese with English abstract)
[7] 宋伟,江俊起,缪勇. 不同玉米品种抗蚜性研究[J]. 生物灾害科学,2014(4):288-292.
SONG W, JIANG J Q,MIAO Y. Studies on resistance of corn varieties to corn aphid[J]. Biological Disaster Science , 2014(4):288-292. (in Chinese with English abstract)
[8] 张衍干,黄吉,施伟迪, 等. 不同玉米品种对玉米蚜的抗性及其与瓢虫的联合控害作用[J]. 浙江农业学报,2016,28(5):815-819.
ZHANG Y G, HUANG J, SHI W D, et al. Resistance to corn aphid( Rhopalosiphum maidis Fitch) of different corn varieties(lines) and its association with predator ladybirds in controlling aphid population[J]. Acta Agriculturae Zhejiangensis , 2016,28(5): 815-819. (in Chinese with English abstract)
[9] 武德功,杜军利,邢素芝, 等. 6个糯玉米品种对玉米蚜的抗性评价[J]. 安徽农业科学,2016,44(12):38-40.
WU D G, DU J L, XIN S Z, et al. Evaluation on resistance of 6 waxy corn cultivars to Rhopalosiphum maidis [J]. Journal of Anhui Agricultural Science ,2016, 44(12) : 38-40. (in Chinese with English abstract)
[10] 王怡,赵曼,田体伟, 等. 不同玉米品种玉米蚜田间消长与其主要生化物质的相关性研究[J]. 河南农业大学学报,2016,50(3):364-369.
WANG Y, ZHAO M, TIAN T W, et al. Study on the relationship between the occurrence quantity of Rhopalosiphum maidis (Fitch) and main biochemical substance contents in maize[J]. Journal of Henan Agricultural University , 2016,50(3):364-369. (in Chinese with English abstract)
[11] 赵文峰,张泽民,付国占, 等. 不同抗性玉米自交系感蚜期四种酶活性变化分析[J]. 山东农业科学,2010(10):46-49.
ZHAO W F, ZHANG Z M, FU G Z, et al. Activity variation of four enzymes in maize inbred lines with different resistance during invaded by aphis[J]. Shandong Agricultural Sciences , 2010(10):46-49. (in Chinese with English abstract)
[12] 高俊凤.植物生理学实验指导[M]. 北京:高等教育出版, 2006.
[13] 孔祥生, 易现锋. 植物生理学实验技术[M]. 北京:中国农业出版社, 2008.
[14] 巫光宏, 何平, 黄卓烈. 生物化学实验技术[M]. 北京:中国农业出版社, 2016.
[15] REN B, LIU W, ZHANG J, et al. Effects of plant density on the photosynthetic and chloroplast characteristics of maize under high-yielding conditions[J]. Die Naturwissenschaften , 2017, 104(3/4): 12.
[16] ZHAO K, WU Y. Effects of Zn deficiency and bicarbonate on the growth and photosynthetic characteristics of four plant species[J]. PLoS One ,2017, 12(1): e0169812.
[17] 黄伟.不同紫花苜蓿品种抗蚜性鉴定及抗蚜性机理初步研究[D].杨凌:西北农林科技大学,2007.
HUANG W. Evaluation of the resistance to aphid of alfalfa varieties and preliminary studies on the resistance mechanism[D]. Yangling: Northwest Agriculture and Forestry University, 2007. (in Chinese with English abstract)
[18] 武德功, 贺春贵, 刘长仲, 等. 不同苜蓿品种对豌豆蚜的生化抗性机制[J]. 草地学报, 2011, 19(3): 498-501.
WU D G, HE C G, LIU C Z, et al. Biochemical resistance mechanism of Medicago sativa to Acyrthosiphon pisum [J]. Acta Agrestia Sinica , 2011, 19(3): 498-501. (in Chinese with English abstract)
[19] 段灿星,彭高松,王晓鸣, 等. 抗感水稻品种受灰飞虱为害后的生理反应差异[J]. 应用昆虫学报,2013,50(1):145-153.
DUAN C X, PENG G S, WANG X M, et al. Differences in the physiological response of resistant and susceptible rice varieties infested by the small brown planthopper[J]. Chinese Journal of Applied Entomology , 2013,50(1): 145-153. (in Chinese with English abstract)
[20] BARBEHENN R V, BERNAYS E A. Relative nutritional quality of C3 and C4 grasses for a graminivorous lepidopteran, Paratrytone melane (Hesperiidae)[J]. Oecologia , 1992, 92(1):97-103.
[21] BROWN A S, SIMMONDS M J, BLANEY W M. Relationship between nutritional composition of plant species and infestation levels of thrips[J]. Journal of Chemical Ecology ,2002,28(12): 2399-2409.
[22] 陈建明, 俞晓平, 葛秀春, 等.水稻植株防御白背飞虱为害的某些生理反应[J]. 中国水稻科学,2000,14(1): 43-47.
CHEN J M, YU X P, GE X C, et al. Some physiological changes of rice plants infested by the white-backed planthopper, Sogatella furcifera [J]. Chinese Journal Rice Science , 2000, 14(1):43-47. (in Chinese with English abstract)
[23] 周福才,陆自强,陈丽芳, 等. 小麦麦株内可溶性糖与抗禾谷缢管蚜的关系[J]. 江苏农业研究,1999, 20(2):60-63.
ZHOU F C, LU Z Q, CHEN L F, et al. The correlativity between soluble sugars of wheat and resistance to the bird cherry-oat aphid Rhopalosiphum padi L.[J]. Jiangsu Agricultural Research ,1999,20(2): 60-63. (in Chinese with English abstract)
[24] 刘玉良,米福贵,特木尔步和,等.苜蓿蓟马抗性与生理活性相关性研究[J].安徽农业科学,2009,37(18):8569-9571,8613.
LIU Y L, MI F G, TE M, et al. Correlation study of Medicago sativa resistance to thrips and physiological activity[J]. Journal of Anhui Agricultural Science , 2009, 37(18):8569-8571,8613. (in Chinese with English abstract)
[25] 黄伟,贾志宽,韩清芳.蚜虫( Aphis medicaginis Koch)危害胁迫对不同苜蓿品种体内丙二醛含量及防御性酶含量的影响[J].生态学报,2007,27(6):2177-2183.
HUANG W, JIA Z K, HAN Q F. Effects of herbivore stress by Aphis medicaginis Koch on the contents of MDA and activities of protective enzymes in different alfalfa varieties[J]. Acta Ecologica Sinica , 2007,27(6):2177-2183. (in Chinese with English abstract)
[26] 武德功,王森山,刘长仲,等. 豌豆蚜刺吸胁迫对不同苜蓿品种体内单宁含量及生理活性的影响[J]. 草地学报,2011, 19(2):351-355.
WU D G, WANG S S, LIU C Z, et al. Effects of herbivore stress by Acyrthosiphon pisum on the contents of tannin and physiological activity in different alfalfa cultivars[J]. Acta Agrestia Sinica , 2011, 19(2): 351-355. (in Chinese with English abstract)
[27] MOLOI M J, VAN DER WESTHUIZEN A J. Antioxidative enzymes and the Russian wheat aphid ( Diuraphis noxia ) resistance response in wheat ( Triticum aestivum )[J]. Plant Biology , 2008,10(3):403-407.
[28] REHMAN R U, ZIA M, CHAUDHARY M F. Salicylic acid and ascorbic acid retrieve activity of antioxidative enzymes and structure of Caralluma tuberculata calli on PEG stress[J]. General Physiology and Biophysics ,2017,36: 167-174.
[29] SAHU B, SAHU A K, CHENNAREDDY S R, et al. Insights on germinability and desiccation tolerance in developing neem seeds ( Azadirachta indica ): Role of AOS, antioxidative enzymes and dehydrin-like protein[J]. Plant Physiology and Biochemistry ,2017,112:64-73.
[30] 张家洋. 重金属及盐胁迫对绿金合果芋生理特性的影响[J]. 浙江农业学报,2016,28(4):601-608.
ZHANG J Y. Effects of heavy metals and salt stress on physiological characteristics of Syngonium podophyllum Schott[J]. Acta Agriculturae Zhejiangensis , 2016,28(4):601-608.(in Chinese with English abstract)
[31] LIU H, ZHANG C, WANG J, et al. Influence and interaction of iron and cadmium on photosynthesis and antioxidative enzymes in two rice cultivars[J]. Chemosphere , 2017,171: 240-247.
[32] HE S, HU Y, WANG H, et al. Effects of indole-3-acetic acid on arsenic uptake and antioxidative enzymes in Pteris cretica var. nervosa and Pteris ensiformis [J]. International Journal of Phytoremediation , 2017, 19(3):231-238.
[33] 赵伶俐,范崇辉,葛红,等.植物多酚氧化酶及其活性特征的研究进展[J].西北林学院学报,2005,20(3):156-159.
ZHAO L L, FAN C H, GE H, et al. Progress on polyphenol oxidase and its activity characteristics in plants[J]. Journal of Northwest Forestry University , 2005,20(3):156-159.(in Chinese with English abstract)
[34] 王曼玲,胡中立,周明全,等.植物多酚氧化酶的研究进展[J].植物学通报,2005,22(2):215-222.
WANG M L, HU Z L, ZHOU M Q, et al. Advances in research of polyphenol oxidase in plants[J]. Chinese Bulletin of Botany ,2005,22(2):215-222.(in Chinese with English abstract)
[35] 黄小贞, 赵德刚. 植物苯丙氨酸解氨酶表达调控机理的研究进展[J].贵州农业科学,2017,45(4):16-20.
HUANG X Z, ZHAO D G. Research progress in regulation and control mechanism of phenylalanine ammonia lyase in plants[J]. Guizhou Agricultural Sciences ,2017,45(4):16-20.(in Chinese with English abstract)
[36] 张丽,常金华,罗耀武.不同高粱基因型感蚜虫前后POD、PPO、PAL酶活性变化分析[J].中国农学通报,2005,21(7):40-42,198.
ZHANG L, CHANG J H, LUO Y W. Activity changes of POD, PPO, PAL of the different sorghum genotypes invaded by Aphis sacchari . Zehntner[J]. Chinese Agricultural Science Bulletin , 2005,21(7):40-42,198.(in Chinese with English abstract)

Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TAN Jingru, HU Qizan, YUE Zhichen, TAO Peng, LEI Juanli, LI Biyuan, ZHAO Yanting, ZANG Yunxiang. Comprehensive evaluation system for heat tolerance of seedling-edible Chinese cabbage (Brassica rapa L. ssp.) based on chlorophyll fluorescence parameters [J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 288-299.
[2]	LIU Yancen, GUO Junxian, GUO Yang, SHI Yong, HUANG Hua, LI Longjie, ZHANG Zhenzhen. Detection of relative chlorophyll content of field cantaloupe canopy at different growth stages based on digital images [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 651-661.
[3]	HU Lixia, ZHANG Jing, GAO Yanqiang, MAO Erye, HAN Kangning, YANG Yan, XIE Jianming. Effects of long-term magnesium stress on chlorophyll fluorescence characteristics and antioxidant properties of celery [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 295-307.
[4]	ZHANG Xujuan, ZHOU Liping, YU Kexin, CHAI Weiguo, PAN Nian, FU Xiaoxia, SHI Jianjun. Determination of annual variation of yield and main nutrients of green asparagus [J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2238-2246.
[5]	ZHOU Lili, FENG Haikuan, NIE Chenwei, XU Xiaobin, LIU Yuan, MENG Lin, XUE Beibei, MING Bo, LIANG Qiyun, SU Tao, JIN Xiuliang. Influence of unmanned aerial vehicle observation time on estimation of canopy chlorophyll density of maize [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 18-31.
[6]	LI Xiaoxia, LI Dan, LI Wanxing, JIN Kunpeng, LIU Yongzhong, HAN Wenqing, HUANG Xuefang, LIU Xin, TIAN Gang, CAO Jinjun. Effects of different rotation patterns on physiological characteristics, yield and quality of foxtail millet during grain filling stage [J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1499-1510.
[7]	ZHANG Xuenan, WANG Lele, NIU Mingxuan, ZHAN Ni, REN Haojie, XU Haocong, YANG Kun, WU Liquan, KE Jian, YOU Cuicui, HE Haibing. Estimation of rice leaf water content based on leaf reflectance spectrum and chlorophyll fluorescence [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1265-1277.
[8]	XIONG Xingwei, WANG Yiqin, TIAN Huaizhi, ZHANG Suqin, GENG Guangdong. Molecular mechanisms of chlorophyll-reduced cotyledon based on transcriptome sequencing in pumpkin [J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 90-102.
[9]	DING Dongxia, LI Nenghui, LI Jing, TANG Chaonan, WANG Cheng, NIU Tianhang, YANG Yan, YANG Haitao, XIE Jianming. Effects of exogenous melatonin on chlorophyll fluorescence and antioxidant system of pepper (Capsicum annuum L.) under low temperature and low light stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1935-1944.
[10]	ZHANG Zhiguo, CONG Lin, ZHANG Shijie, LI Rongguang, ZOU Weina, CHI Fa'an, ZHANG Bao, JIANG Yuping. Effects of root-zone temperature on growth, development and flowering of Hemerocallis fulva [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1005-1014.
[11]	WANG Huiru, LI Jianshe, YAN Sihua, GAO Yanming. Effect of prunning patterns on canopy light interception characteristics and chlorophyll fluorescence parameters in cherry tomato [J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 525-533.
[12]	CAI Yao, MIAO Yuxuan, WU Hao, WANG Dan. Hyperspectral characteristics and leaf area index (LAI) and SPAD value inversion of winter wheat under elevated CO₂ concentration [J]. Acta Agriculturae Zhejiangensis, 2022, 34(3): 582-589.
[13]	SUN Shouxia, CHEN Hong, LYU Wei, PIAO Hanqi, ZHOU Guanghui, WANG Hesong, ZHANG Shubin, HAO Jinlian. Effects of dustfall on photosynthetic characteristics and chlorophyll fluorescence characteristics of major fruit trees in Aksu Region, China [J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2659-2668.
[14]	FENG Caijun, SONG Ruijiao, SONG Lingyu, ZHANG Song, QI Juncang. Effects of soaking seeds with 2, 4-epbrassinolide on starch netabolism during germination of barley seeds under drought stress [J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2112-2120.
[15]	WANG Jia, MU Ruirui, YANG Qiaoqiao, LIU Wei, ZHANG Yuehe, KANG Jianhong. Effects of potassium application rate on chlorophyll fluorescence characteristics and yield of spring maize in Ningxia under integrated drip irrigation [J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1347-1357.