Effects of earthworm extract on heat shock protein levels and immune function of silkworm, Bombyx mori

doi:10.3969/j.issn.1004-1524.2020.04.05

›› 2020, Vol. 32 ›› Issue (4): 593-600.DOI: 10.3969/j.issn.1004-1524.2020.04.05

• Animal Science • Previous Articles Next Articles

Effects of earthworm extract on heat shock protein levels and immune function of silkworm, Bombyx mori

XU Qilin¹, QI Guilan¹, MO Guilin², ZHU Jiang³, LEI Chunlong¹, WU Yongsheng^1,*, XU Zhenying¹, LI Juan¹

1.Institute of Animal Science, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China;
2.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
3.Sericultural Bureau of Lezhi, Lezhi 641500, China

Received:2019-09-29 Online:2020-04-25 Published:2020-04-26

Abstract

Abstract: To investigate the effect of earthworm extract on the expression of heat shock protein and immune function of silkworm, Bombyx mori, the fifth instar larvae of silkworm were fed with mulberry leaves to which one of three diluted does (1/50, 1/100 and 1/200) earthworm extract had been added. The midgut and hemolymph were obtained after feeding for six days, the expression of HSP70 and HSP90 in midgut were analyzed by quantitative real-time PCR and the levels of HSP70, HSP90, lysozyme and attacin in the midgut and hemolymph were tested by the ELISA kit. The results showed that, the expression of HSP70 gene in the midgut tissues of the 1/100 and 1/200 dilution groups was 2.97 and 1.86 times of the control group, respectively (P<0.05). The expression of HSP90 gene in the midgut tissues of the 1/100 dilution group was 2.2 times of the control group (P<0.05). The HSP70 and hemolymph peptides in the 1/50 dilution group were significantly higher than those in the control group (P<0.05). There was no significant difference in HSP90 between intestine and hemolymph in each treatment group (P>0.05).Thus, adding the earthworm extract in mulberry leaves can promote the expression of heat shock protein, increase the level of atticin, and improve the resistance and immunity of silkworm.

Key words: earthworm extract, silkworm, heat shock protein, quantitative real-time PCR, lysozyme, attacin

CLC Number:

S881.2

XU Qilin, QI Guilan, MO Guilin, ZHU Jiang, LEI Chunlong, WU Yongsheng, XU Zhenying, LI Juan. Effects of earthworm extract on heat shock protein levels and immune function of silkworm, Bombyx mori[J]. , 2020, 32(4): 593-600.

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Effects of earthworm extract on heat shock protein levels and immune function of silkworm, Bombyx mori

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