Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis

doi:10.3969/j.issn.1004-1524.2020.07.02

›› 2020, Vol. 32 ›› Issue (7): 1149-1154.DOI: 10.3969/j.issn.1004-1524.2020.07.02

• Animal Science • Previous Articles Next Articles

Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis

DU Yanbin, ZHANG Gangchen, WANG Yuxin, LIU Baobao, GONG Shenglong, DONG Xiao, WANG Yang^*

Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received:2019-10-23 Online:2020-07-25 Published:2020-07-28

Abstract

Abstract: This study aimed to clone the rpoE gene of Streptococcus suis and to analyze the function and related biological characteristics of the rpoE gene encoding protein of Streptococcus suis by bioinformatic software. The rpoE gene was amplified from Streptococcus suis and sequenced; the physicochemical properties and antigenic epitopes of the protein encoded by the gene were predicted by bioinformatic software. The results showed that the rpoE gene of Streptococcus suis was 582 bp in length, encoding a hydrophilic cytoplasmic protein rich in glutamic acid, aspartic acid and serine. Besides, the secondary structure analysis showed that the main structural components of RpoE protein were α-helix and random coil, accounting for 52.3% and 36.3%, respectively. The comprehensive analysis indicates that there are 5 dominant epitopes on RpoE protein.

Key words: Streptococcus suis, RpoE, clone, bioinformatic analysis

CLC Number:

S855.1⁺1

DU Yanbin, ZHANG Gangchen, WANG Yuxin, LIU Baobao, GONG Shenglong, DONG Xiao, WANG Yang. Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis[J]. , 2020, 32(7): 1149-1154.

References

[1] 汪洋. 猪链球菌生物被膜形成及致病机理研究[D]. 南京: 南京农业大学, 2011.
WANG Y.Study on the mechanism of biofilm formation and molecular pathogenesis of Streptococcus suis biofilm[D]. Nanjing: Nanjing Agricultural University, 2011.(in Chinese with English abstract)
[2] GOYETTE-DESJARDINS G, AUGER J P, XU J G, et al.Streptococcus suis, an important pig pathogen and emerging zoonotic agent: an update on the worldwide distribution based on serotyping and sequence typing[J]. Emerging Microbes & Infections, 2014, 3(1): 1-20.
[3] DUTKIEWICZ J, SROKA J, ZAJ?C V, et al. Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I: epidemiology[J]. Annals of Agricultural and Environmental Medicine, 2017, 24(4): 683-695.
[4] XUE X L, TOMASCH J, SZTAJER H, et al.The delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation[J]. Journal of Bacteriology, 2010, 192(19): 5081-5092.
[5] XUE X L, LI J S, WANG W, et al.The global impact of the delta subunit RpoE of the RNA polymerase on the proteome of Streptococcus mutans[J]. Microbiology, 2012, 158(1): 191-206.
[6] GU D, GUO M, YANG M J, et al.A σE-mediated temperature gauge controls a switch from LuxR-mediated virulence gene expression to thermal stress adaptation in Vibrio alginolyticus[J]. PLoS Pathogens, 2016, 12(6): e1005645.
[7] EGLER M, GROSSE C, GRASS G, et al.Role of the extracytoplasmic function protein family sigma factor RpoE in metal resistance of Escherichia coli[J]. Journal of Bacteriology, 2005, 187(7): 2297-2307.
[8] BARBIER M, BOEHM D T, SEN-KILIC E, et al.Modulation of pertussis and adenylate cyclase toxins by sigma factor RpoE in Bordetella pertussis[J]. Infection and Immunity, 2017, 85(1): e00565.
[9] DOU Y, RUTANHIRA H, CHEN X, et al.Role of extracytoplasmic function sigma factor PG1660 (RpoE) in the oxidative stress resistance regulatory network of Porphyromonas gingivalis[J]. Molecular Oral Microbiology, 2018, 33(1): 89-104.
[10] HUMPHREYS S, STEVENSON A, BACON A, et al.The alternative sigma factor, VE, is critically important for the virulence of Salmonella typhimurium[J]. Infection and Immunity, 1999, 67(4): 1560-1568.
[11] 张高峰, 杨侃侃, 张谦, 等. 羊口疮病毒安徽株023基因的原核表达与生物信息学分析[J]. 浙江农业学报, 2019, 31(1): 30-38.
ZHANG G F, YANG K K, ZHANG Q, et al.Prokaryotic expression and bioinformatics analysis of orf virus Anhui strain 023 gene[J]. Acta Agriculturae Zhejiangensis, 2019, 31(1): 30-38.(in Chinese with English abstract)
[12] SZKLARCZYK D, MORRIS J H, COOK H, et al.The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible[J]. Nucleic Acids Research, 2017, 45(D1): D362-D368.
[13] YONGKIETTRAKUL S, MANEERAT K, ARECHANAJAN B, et al.Antimicrobial susceptibility of Streptococcus suis isolated from diseased pigs, asymptomatic pigs, and human patients in Thailand[J]. BMC Veterinary Research, 2019, 15: 5.
[14] SEEPERSAUD R, NEEDHAM R H V, KIM C S, et al. Abundance of the δ subunit of RNA polymerase is linked to the virulence of Streptococcus agalactiae[J]. Journal of Bacteriology, 2006, 188(6): 2096-2105.
[15] BURTON S P, BURTON Z F.The σ enigma: bacterial σ factors, archaeal TFB and eukaryotic TFIIB are homologs[J]. Transcription, 2014, 5(4): e967599.
[16] LI J, OVERALL C C, NAKAYASU E S, et al.Analysis of the Salmonella regulatory network suggests involvement of SsrB and H-NS in σ^E-regulated SPI-2 gene expression[J]. Frontiers in Microbiology, 2015, 6: 27.
[17] KLEIN G, STUPAK A, BIERNACKA D, et al.Multiple transcriptional factors regulate transcription of the rpoE Gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective[J]. Journal of Biological Chemistry, 2016, 291(44): 22999-23019.
[18] COSTANZO A, NICOLOFF H, BARCHINGER S E, et al.PpGpp and DksA likely regulate the activity of the extracytoplasmic stress factor σE in Escherichia coli by both direct and indirect mechanisms[J]. Molecular Microbiology, 2008, 67(3): 619-632.
[19] KAMARTHAPU V, EPSHTEIN V, BENJAMIN B, et al.PpGpp couples transcription to DNA repair in E. coli[J]. Science, 2016, 352(6288): 993-996.
[20] YAKHNIN H, AICHELE R, ADES S E, et al.Circuitry linking the global csr-and σE-dependent cell envelope stress response systems[J]. Journal of Bacteriology, 2017, 199(23): e00484.

Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HE Jiaqi, ZHAI Ying, ZHANG Jun, QIU Shuang, LI Mingyang, ZHAO Yan, ZHANG Meijuan, MA Tianyi. Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 1-7.
[2]	WANG Weike, LU Na, YAN Jing, SONG Jiling, YUAN Weidong, ZHOU Zufa. Cloning and expression analysis of PpSAMS gene of Pleurotus pulmonarius [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 62-68.
[3]	DUAN Qianqian, MAO Tianjiao, HAN Yeqin, HAN Xuejiao, WEI Jianzhong, SUN Pei, LI Yu. Evaluation of immune efficacy of inactivated Streptococcus suis serotype 2 vaccine in mice [J]. , 2020, 32(4): 577-585.
[4]	CHEN Zhang, WU Huajian, MAO Tianjiao, HAN Yeqin, SUN Pei, WEI Jianzhong, LI Dongfeng, LI Yu. Screening of strain for inactivated vaccine of Streptococcus suis type 2 [J]. , 2020, 32(1): 57-64.
[5]	ZHANG Haojie, LIU Mei, LI Chunyan, HE Ran, LAN Jingchao, LUO Li, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic expression and immunofluorescence localization of nucleoside diphosphate kinase gene from Dirofilaria immitis [J]. , 2019, 31(9): 1453-1460.
[6]	LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila [J]. , 2019, 31(9): 1461-1470.
[7]	LIU Shili, JIA Yongyi, JIANG Wenping, CHI Meili, CHENG Shun, ZHAO Jinliang, GU Zhimin, FU Jianjun. Cloning and sequence analysis of growth hormone gene and its flanking region in topmouth culter (Culter alburnus Basilewsky) [J]. , 2017, 29(8): 1281-1289.
[8]	ZHANG Li, LIU Lixia, LI Qiangzi, CHEN Hong. Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak [J]. , 2017, 29(4): 618-624.
[9]	JIN Weiwei， CHEN Gongkai*， ZHU Jianjun， GAO Ailing. Gene clone， sequence analysis and gene expression of NIN and Sus from Ougan fruit [J]. , 2016, 28(5): 782-.
[10]	ZHAO Ying\\|jie1，LIU Chun\\|lin2，RUAN Ying1，*. Cloning of AtSb10 gene in Arabidopsis thaliana and over\\|expression of AtSb10 in Arabidopsis thaliana#br# [J]. , 2015, 27(9): 1550-.
[11]	CHENG Chen1，2， WANG Jing1，2， YUAN Wen\\|xia1，2， LI Dong\\|yue1，2，YANG Yong1，2，YAN Cheng\\|qi1，2， CHEN Jian\\|ping1，2，*. Research progress of disease\\|related genes in rice lesion mimic mutants [J]. , 2015, 27(7): 1294-.
[12]	YUN Tao， CHEN Liu， ZHANG Cun*， NI Zheng， HUA Jiong\\|gang， YU Bin， YE Wei\\|cheng. Construction and analysis of full length cDNA of duck Tembusu Virus [J]. , 2015, 27(11): 1910-.
[13]	LI Cui\\|xin， ZHANG Wan\\|jie， WANG Bing， HE De*. Molecular identification of wild Pleurotus abalonus from Kunming by rDNA ITS sequences [J]. , 2014, 26(5): 1202-.
[14]	ZHANG Yong;ZHANG Zhen;QIU Hai-ping;WANG Yan-li;WANG Jiao-yu;SUN Guo-chang;. Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui* [J]. , 2010, 22(6): 716-721.
[15]	LIU Jian-xin;ZHANG Zhi;DING Hua-qiao;GE Ya-ying;WANG Wei-yong. Cloning and sequence analysis of GAPDH gene from Guzmania [J]. , 2010, 22(6): 722-726.