Study on epibrassinolide (EBR) on growth and physiological response of Summer Black grape seedlings

doi:10.3969/j.issn.1004-1524.2021.10.12

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (10): 1889-1896.DOI: 10.3969/j.issn.1004-1524.2021.10.12

• Horticultural Science • Previous Articles Next Articles

Study on epibrassinolide (EBR) on growth and physiological response of Summer Black grape seedlings

LIAN Huashan¹(), LI Xinxin², LIN Lijin³, LIAO Ming’an⁴^,^*()

1. School of Agriculture and Horticulture, Chengdu Agricultural College, Chengdu 611130, China
2. Experimental Middle School Attached to Sichuan Normal University, Chengdu 611130, China
3. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China
4. College of Horticulture,Sichuan Agricultural University, Chengdu 611130, China

Received:2020-12-15 Online:2021-10-25 Published:2021-11-02
Contact: LIAO Ming’an

Abstract

Abstract:

In order to study the effect of foliar application of epibrassinolide (EBR) on growth of grape seedlings. A pot experiment was conducted to determine the effect of EBR on biomass, photosynthetic characteristics and antioxidant system of Summer Black grape seedlings by foliar spraying with different concentrations of EBR (0, 0.5, 1.0, 1.5, 2.0 mg·L^-1).The results showed that foliar application of different concentrations of EBR increased the biomass and root shoot ratio of grape seedlings, and photosynthetic pigment content showed the same trend, but the chlorophyll a/b decreased; In addition to the decrease of vapor pressure deficit at leaf temperature, EBR increased photosynthetic parameters (photosynthetic rate, conductance stomatal, transpiration rate, intercellular CO₂ concentration) of seedling leaves;Activities of antioxidant enzymes, contents of free proline and soluble protein in leaves of Summer Blackgrape seedlings were increased by different concentrations of EBR, the better performance was 1.0 mg·L^-1 and 1.5 mg·L^-1. When EBR concentration reached 1.0 mg·L^-1 and 1.5 mg·L^-1, relative conductivity of the leaves was reduced; When EBR concentration was 1.5 mg·L^-1, soluble sugar content of roots and stems of seedlings was improved; When EBR concentration reached 0.5 mg·L^-1 and 1.0 mg·L^-1, soluble sugar content of seedling leaves was increased. In general, foliar spraying of 1.0 mg·L^-1 EBR could promote growth of Summer Black grape seedlings and increase the antioxidant capacity of the seedlings.

Key words: epibrassinolide, Vitis vinifera L., biomass, photosynthesis, antioxidant system

CLC Number:

S663.1

LIAN Huashan, LI Xinxin, LIN Lijin, LIAO Ming’an. Study on epibrassinolide (EBR) on growth and physiological response of Summer Black grape seedlings[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1889-1896.

Figures/Tables 6

References 33

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EBR浓度 EBR concentration/ (mg·L^-1)	生物量Biomass/(g·plant^-1)					根冠比 Root/shoot ratio
EBR浓度 EBR concentration/ (mg·L^-1)	根 Root	茎 Stem	叶 Leaf	地上部 Shoot	整株 Total plant	根冠比 Root/shoot ratio
0	0.555±0.003 e	0.139±0.006 d	1.158±0.044 b	1.297±0.038 d	1.852±0.041 d	0.428 d
0.5	0.773±0.005 d	0.193±0.008 c	1.231±0.057 b	1.423±0.052 c	2.197±0.050 c	0.543 a
1.0	0.864±0.004 a	0.289±0.003 a	1.428±0.025 a	1.718±0.027 ab	2.582±0.028 a	0.503 b
1.5	0.844±0.003 b	0.298±0.007 a	1.497±0.028 a	1.796±0.033 a	2.639±0.036 a	0.470 c
2.0	0.791±0.007 c	0.223±0.009 b	1.439±0.047 a	1.662±0.051 b	2.453±0.057 b	0.476 c

EBR浓度 EBR concentration/ (mg·L^-1)	生物量Biomass/(g·plant^-1)					根冠比 Root/shoot ratio
EBR浓度 EBR concentration/ (mg·L^-1)	根 Root	茎 Stem	叶 Leaf	地上部 Shoot	整株 Total plant	根冠比 Root/shoot ratio
0	0.555±0.003 e	0.139±0.006 d	1.158±0.044 b	1.297±0.038 d	1.852±0.041 d	0.428 d
0.5	0.773±0.005 d	0.193±0.008 c	1.231±0.057 b	1.423±0.052 c	2.197±0.050 c	0.543 a
1.0	0.864±0.004 a	0.289±0.003 a	1.428±0.025 a	1.718±0.027 ab	2.582±0.028 a	0.503 b
1.5	0.844±0.003 b	0.298±0.007 a	1.497±0.028 a	1.796±0.033 a	2.639±0.036 a	0.470 c
2.0	0.791±0.007 c	0.223±0.009 b	1.439±0.047 a	1.662±0.051 b	2.453±0.057 b	0.476 c

EBR浓度 EBR concentration/ (mg·L^-1)	叶绿素a Chlorophyll a/ (mg·g^-1)	叶绿素b Chlorophyll b/ (mg·g^-1)	类胡萝卜素 Carotinoid/ (mg·g^-1)	总叶绿素 Total chlorophyll/ (mg·g^-1)	叶绿素a/b Chlorophyll a/b
0	0.729±0.007 d	0.235±0.004 d	0.157±0.002 c	0.964±0.005 e	3.102 a
0.5	0.818±0.006 b	0.279±0.004 b	0.159±0.008 c	1.155±0.002 c	2.932 b
1.0	0.970±0.008 a	0.332±0.008 a	0.172±0.005 b	1.226±0.023 ab	2.922 b
1.5	0.743±0.002 cd	0.254±0.002 c	0.183±0.002 a	1.171±0.025 bc	2.925 b
2.0	0.749±0.005 c	0.264±0.008 c	0.178±0.006 ab	1.252±0.037 a	2.837 c

EBR浓度 EBR concentration/ (mg·L^-1)	叶绿素a Chlorophyll a/ (mg·g^-1)	叶绿素b Chlorophyll b/ (mg·g^-1)	类胡萝卜素 Carotinoid/ (mg·g^-1)	总叶绿素 Total chlorophyll/ (mg·g^-1)	叶绿素a/b Chlorophyll a/b
0	0.729±0.007 d	0.235±0.004 d	0.157±0.002 c	0.964±0.005 e	3.102 a
0.5	0.818±0.006 b	0.279±0.004 b	0.159±0.008 c	1.155±0.002 c	2.932 b
1.0	0.970±0.008 a	0.332±0.008 a	0.172±0.005 b	1.226±0.023 ab	2.922 b
1.5	0.743±0.002 cd	0.254±0.002 c	0.183±0.002 a	1.171±0.025 bc	2.925 b
2.0	0.749±0.005 c	0.264±0.008 c	0.178±0.006 ab	1.252±0.037 a	2.837 c

EBR浓度 EBR concentration/(mg·L^-1)	T_r/(mmol· m^-2·s^-1)	P_n/(μmol· m^-2·s^-1)	C_i/(μmol· mol^-1)	G_s/(mol· m^-2·s^-1)	VpdL/kPa
0	2.614±0.049 d	3.495±0.118 d	251.7±1.5 d	0.128±0.006 c	2.254±0.039 a
0.5	3.343±0.072 a	7.182±0.086 b	319.7±6.1 b	0.184±0.005 a	1.632±0.015 d
1.0	2.980±0.037 c	7.173±0.191 b	301.0±5.0 c	0.150±0.006 b	1.952±0.040 c
1.5	3.294±0.070 a	9.019±0.062 a	351.8±2.5 a	0.192±0.004 a	2.060±0.016 b
2.0	3.107±0.010 b	6.838±0.045 c	296.1±2.8 c	0.143±0.006 b	1.365±0.047 e

Study on epibrassinolide (EBR) on growth and physiological response of Summer Black grape seedlings

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EBR浓度 EBR concentration/(mg·L^-1)	SOD活性 SOD activity/(U·g^-1)	POD活性 POD activity/(U·g^-1·min^-1)	CAT活性 CAT activity/(mg·g^-1·min^-1)
0	72.80±2.20 c	14.23±0.54 d	0.622±0.009 d
0.5	114.10±3.3 b	29.64±0.79 b	0.800±0.008 c
1.0	124.30±4.2 a	31.77±0.29 a	0.950±0.004 a
1.5	117.10±3.2 b	31.39±0.92 a	0.857±0.004 b
2.0	75.50±1.60 c	23.81±0.42 c	0.846±0.007 b

EBR浓度 EBR concentration/ (mg·L^-1)	丙二醛 MDA content/ (μmol·kg^-1)	脯氨酸 Proline content/ (μg·g^-1)	可溶性蛋白质 Soluble protein content/(mg·g^-1)	相对电导率 Relative conductivity/ (μS·cm^-1)
0	56.34±0.47 a	52.89±0.64 d	5.825±0.070 c	96.66±0.75 c
0.5	46.59±0.52 c	64.76±0.61 c	8.618±0.662 ab	102.70±0.40 a
1.0	31.69±0.36 e	79.81±0.49 a	8.754±0.367 a	95.20±0.70 d
1.5	33.44±0.21 d	70.29±0.74 b	6.453±0.903 c	86.93±0.72 e
2.0	48.87±0.75 b	53.29±0.21 d	7.593±0.130 b	99.77±0.91 b

EBR浓度 EBR concentration/(mg·L^-1)	根Root	茎Stem	叶Leaf
0	54.74±4.20 d	83.62±5.20 c	91.76±1.40 c
0.5	51.72±4.20 e	83.09±8.90 c	95.63±5.10 b
1.0	59.46±3.00 c	74.83±2.40 d	126.80±6.7 a
1.5	62.24±1.70 b	90.24±6.00 b	78.15±3.30 e
2.0	87.35±4.80 a	95.35±8.80 a	82.91±5.80 d

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