Construction of DNA fingerprint for 48 broccoli cultivars

doi:10.3969/j.issn.1004-1524.2021.12.10

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2304-2312.DOI: 10.3969/j.issn.1004-1524.2021.12.10

• Horticultural Science • Previous Articles Next Articles

Construction of DNA fingerprint for 48 broccoli cultivars

LIN Mengjie¹^,²(), WEN Huiping¹, XIAO Jianzhong¹, ZHENG Qiang¹^,^*()

1. College of Ecology, Lishui University, Lishui 323000, China
2. College of Life Science, South China Normal University, Guangzhou 510631, China

Received:2020-12-29 Online:2021-12-25 Published:2022-01-10
Contact: ZHENG Qiang

Abstract

Abstract:

To screen a set of core SSR (simple sequence repeat) primers for identifying of broccoli cultivars, 12 genetically different broccoli varieties were chosen as screening materials, combined polyacrylamide gel electrophoresis and fluorescence capillary electrophoresis techniques, 20 pairs of core SSR primers were finally selected from a total of 945 pairs of Brassica SSR primers. Using these 20 pairs of core primers, 79 polymorphic alleles were amplified from 48 common broccoli cultivars, with 3.95 polymorphic alleles and 5.55 genotypes per pair of primer in average. The polymorphism information content (PIC) value ranged from 0.302 to 0.750, with an average of 0.547, and the heterozygosity ranged from 0.350 0 to 0.784 9, with an average value of 0.608 2. Each of the fingerprints of 48 broccoli cultivars constructed with the core SSR primers was unique and could be used to characterize one cultivar, which provided a scientific basis for broccoli variety identification and resource protection.

Key words: broccoli, simple sequence repeat molecular marker, fingerprint, allele, polymorphism information content

CLC Number:

S635

LIN Mengjie, WEN Huiping, XIAO Jianzhong, ZHENG Qiang. Construction of DNA fingerprint for 48 broccoli cultivars[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2304-2312.

Figures/Tables 6

References 22

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编号 Code	品种名称 Cultivar name	性状 Characteristics	来源 Origin	编号 code	品种名称 Cultivar name	性状 Characteristics	来源 Origin
B1	炎秀 Enlish name unknown	中熟,耐热 Medium maturity, heat tolerance	日本 Japan	B25	绿新1号 Lyuxin 1	早熟 Early maturity	中国 China
B2	优秀 Enlish name unknown	早熟 Early maturity	日本 Japan	B26	绿新55 Lyuxin 55	中早熟 Middle-early maturity	中国 China
B3	耐寒优秀 Enlish name unknown	中早熟,耐寒 Middle-early maturity, cold tolerance	日本 Japan	B27	福绿 Fulyu	中晚熟,不耐寒 Middle-late maturity, non-cold tolerance	中国 China
B4	阳光 Enlish name unknown	中早熟 Middle-early maturity	日本 Japan	B28	园博园 Yuanboyuan	中早熟 Middle-early maturity	中国 China
B5	喜鹊 Enlish name unknown	中早熟 Middle-early maturity	日本 Japan	B29	奇秀 Qixiu	中晚熟 Middle-late maturity	中国 China
B6	幸运1705 bejo1705	早熟 Early maturity	荷兰 Holland	B30	奇绿 Qilyu	中晚熟 Middle-late maturity	中国 China
B7	黛兰诺85 Covina	中熟 Medium maturity	荷兰 Holland	B31	绿剑100 Lyujian 100	中晚熟 Middle-late maturity	中国 China
B8	B1199	中熟 Medium maturity	美国 America	B32	绿剑2号 Lyujian 2	中熟 Medium maturity	中国 China
B9	SV1808	中晚熟 Middle-late maturity	美国 America	B33	绿剑5号 Lyujian 5	中熟 Medium maturity	中国 China
B10	南秀366 Contriubtor	中熟 Medium maturity	美国 America	B34	黄金绿富士 Huangjinlyufushi	中晚熟 Middle-late maturity	中国 China
B11	万宝金 Wanbaojin	中熟,耐热 Medium maturity, heat tolerance	中国 China	B35	马尼拉 Manila	中熟 Medium maturity	中国 China
B12	TBR-800	中晚熟 Middle-late maturity	日本 Japan	B36	绿宝石 Lyubaoshi	中熟 Medium maturity	中国 China
B13	Tatuno-3	中晚熟 Middle-late maturity	日本 Japan	B37	绿冠304 Lyuguan 304	中早熟 Middle-early maturity	中国 China
B14	绿雄90 TSX-8720	中晚熟 Middle-late maturity	日本 Japan	B38	绿冠305 Lyuguan 305	中熟 Medium maturity	中国 China
B15	多福70 Duofu70	中早熟 Middle-early maturity	中国 China	B39	绿冠307 Lyuguan 307	中晚熟,抗病 Middle-late maturity, disease resistance	中国 China
B16	蔓陀绿 Montop	早熟 Early maturity	瑞士 Switzerland	B40	绿冠90 Lyuguan 90	晚熟 Late maturity	中国 China
B17	撒利奥55 SJD4149	早熟 Early maturity	瑞士 Switzerland	B41	文兴 Wenxing	中熟 Medium maturity	中国 China
B18	撒利奥887 Montevidoe	晚熟 Late maturity	瑞士 Switzerland	B42	翠绿90 Cuilyu 90	晚熟 Late maturity	中国 China
B19	台绿1号 Tailv 1	中晚熟 Middle-late maturity	中国 China	B43	绿丽80 Lyuli 80	中熟 Medium maturity	中国 China
B20	台绿2号 Tailv 2	早熟 Early maturity	中国 China	B44	浙青95 Zheqing 95	晚熟 Late maturity	中国 China
B21	台绿5号 Tailv 5	晚熟 Late maturity	中国 China	B45	艳秀 Yanxiu	中早熟 Middle-early maturity	中国 China
B22	青花65 Qinghua 65	中早熟 Middle-early maturity	中国 China	B46	万宝绿 Wanbaolyu	中早熟,耐寒 Middle-early maturity, cold tolerance	中国 China
B23	马拉松 Enlish name unknown	中晚熟 Middle-late maturity	韩国 Korea	B47	台绿3号 Tailyu 3	中熟 Medium maturity	中国 China
B24	青鹤 Enlish name unknown	中晚熟 Middle-late maturity	韩国 Korea	B48	中青8号 Zhongqing 8	中早熟 Middle-early maturity	中国 China

编号 Code	品种名称 Cultivar name	性状 Characteristics	来源 Origin	编号 code	品种名称 Cultivar name	性状 Characteristics	来源 Origin
B1	炎秀 Enlish name unknown	中熟,耐热 Medium maturity, heat tolerance	日本 Japan	B25	绿新1号 Lyuxin 1	早熟 Early maturity	中国 China
B2	优秀 Enlish name unknown	早熟 Early maturity	日本 Japan	B26	绿新55 Lyuxin 55	中早熟 Middle-early maturity	中国 China
B3	耐寒优秀 Enlish name unknown	中早熟,耐寒 Middle-early maturity, cold tolerance	日本 Japan	B27	福绿 Fulyu	中晚熟,不耐寒 Middle-late maturity, non-cold tolerance	中国 China
B4	阳光 Enlish name unknown	中早熟 Middle-early maturity	日本 Japan	B28	园博园 Yuanboyuan	中早熟 Middle-early maturity	中国 China
B5	喜鹊 Enlish name unknown	中早熟 Middle-early maturity	日本 Japan	B29	奇秀 Qixiu	中晚熟 Middle-late maturity	中国 China
B6	幸运1705 bejo1705	早熟 Early maturity	荷兰 Holland	B30	奇绿 Qilyu	中晚熟 Middle-late maturity	中国 China
B7	黛兰诺85 Covina	中熟 Medium maturity	荷兰 Holland	B31	绿剑100 Lyujian 100	中晚熟 Middle-late maturity	中国 China
B8	B1199	中熟 Medium maturity	美国 America	B32	绿剑2号 Lyujian 2	中熟 Medium maturity	中国 China
B9	SV1808	中晚熟 Middle-late maturity	美国 America	B33	绿剑5号 Lyujian 5	中熟 Medium maturity	中国 China
B10	南秀366 Contriubtor	中熟 Medium maturity	美国 America	B34	黄金绿富士 Huangjinlyufushi	中晚熟 Middle-late maturity	中国 China
B11	万宝金 Wanbaojin	中熟,耐热 Medium maturity, heat tolerance	中国 China	B35	马尼拉 Manila	中熟 Medium maturity	中国 China
B12	TBR-800	中晚熟 Middle-late maturity	日本 Japan	B36	绿宝石 Lyubaoshi	中熟 Medium maturity	中国 China
B13	Tatuno-3	中晚熟 Middle-late maturity	日本 Japan	B37	绿冠304 Lyuguan 304	中早熟 Middle-early maturity	中国 China
B14	绿雄90 TSX-8720	中晚熟 Middle-late maturity	日本 Japan	B38	绿冠305 Lyuguan 305	中熟 Medium maturity	中国 China
B15	多福70 Duofu70	中早熟 Middle-early maturity	中国 China	B39	绿冠307 Lyuguan 307	中晚熟,抗病 Middle-late maturity, disease resistance	中国 China
B16	蔓陀绿 Montop	早熟 Early maturity	瑞士 Switzerland	B40	绿冠90 Lyuguan 90	晚熟 Late maturity	中国 China
B17	撒利奥55 SJD4149	早熟 Early maturity	瑞士 Switzerland	B41	文兴 Wenxing	中熟 Medium maturity	中国 China
B18	撒利奥887 Montevidoe	晚熟 Late maturity	瑞士 Switzerland	B42	翠绿90 Cuilyu 90	晚熟 Late maturity	中国 China
B19	台绿1号 Tailv 1	中晚熟 Middle-late maturity	中国 China	B43	绿丽80 Lyuli 80	中熟 Medium maturity	中国 China
B20	台绿2号 Tailv 2	早熟 Early maturity	中国 China	B44	浙青95 Zheqing 95	晚熟 Late maturity	中国 China
B21	台绿5号 Tailv 5	晚熟 Late maturity	中国 China	B45	艳秀 Yanxiu	中早熟 Middle-early maturity	中国 China
B22	青花65 Qinghua 65	中早熟 Middle-early maturity	中国 China	B46	万宝绿 Wanbaolyu	中早熟,耐寒 Middle-early maturity, cold tolerance	中国 China
B23	马拉松 Enlish name unknown	中晚熟 Middle-late maturity	韩国 Korea	B47	台绿3号 Tailyu 3	中熟 Medium maturity	中国 China
B24	青鹤 Enlish name unknown	中晚熟 Middle-late maturity	韩国 Korea	B48	中青8号 Zhongqing 8	中早熟 Middle-early maturity	中国 China

引物 Primer	染色体位置 Chromosomal location	等位基因数量 Alleles No.	基因型数量 Genotype No.	杂合度 Heterozygosity	PIC值 PIC value
Na10H03c	C1	3	5	0.561 0	0.477
BnEMS0886	C1	3	6	0.654 3	0.580
BoGMS1162	C1	4	5	0.488 7	0.451
BoSF239	C2	3	3	0.451 2	0.349
BoSF2725	C2	4	5	0.665 8	0.612
MR049B	C3	2	3	0.375 0	0.304
BRMSo71	C3	5	5	0.682 1	0.628
BoE718	C3	5	10	0.722 9	0.672
sORF74	C4	5	8	0.620 0	0.577
CB10288	C4	5	6	0.696 4	0.646
BoSF259	C5	3	4	0.617 0	0.537
BoGMS0949	C5	3	6	0.637 8	0.565
Na12A02	C6	4	5	0.613 1	0.566
CB10234	C6	5	6	0.738 5	0.697
CNU400	C7	5	6	0.784 9	0.750
BoE723	C7	4	5	0.693 8	0.637
CB10139	C8	4	9	0.708 1	0.657
CB10028	C8	2	3	0.468 8	0.359
CB10064	C9	6	7	0.635 2	0.572
BoSF2686	C9	4	4	0.350 0	0.302

引物 Primer	染色体位置 Chromosomal location	等位基因数量 Alleles No.	基因型数量 Genotype No.	杂合度 Heterozygosity	PIC值 PIC value
Na10H03c	C1	3	5	0.561 0	0.477
BnEMS0886	C1	3	6	0.654 3	0.580
BoGMS1162	C1	4	5	0.488 7	0.451
BoSF239	C2	3	3	0.451 2	0.349
BoSF2725	C2	4	5	0.665 8	0.612
MR049B	C3	2	3	0.375 0	0.304
BRMSo71	C3	5	5	0.682 1	0.628
BoE718	C3	5	10	0.722 9	0.672
sORF74	C4	5	8	0.620 0	0.577
CB10288	C4	5	6	0.696 4	0.646
BoSF259	C5	3	4	0.617 0	0.537
BoGMS0949	C5	3	6	0.637 8	0.565
Na12A02	C6	4	5	0.613 1	0.566
CB10234	C6	5	6	0.738 5	0.697
CNU400	C7	5	6	0.784 9	0.750
BoE723	C7	4	5	0.693 8	0.637
CB10139	C8	4	9	0.708 1	0.657
CB10028	C8	2	3	0.468 8	0.359
CB10064	C9	6	7	0.635 2	0.572
BoSF2686	C9	4	4	0.350 0	0.302

Construction of DNA fingerprint for 48 broccoli cultivars

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