Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora

doi:10.3969/j.issn.1004-1524.2022.10.16

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (10): 2220-2229.DOI: 10.3969/j.issn.1004-1524.2022.10.16

• Horticultural Science • Previous Articles Next Articles

Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora

HOU Jinghan¹(), WAN Yingling¹, LIU Aiqing², HONG Aiying², LIU Yan¹^,^*()

1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
2. Urban Management Development Center of Heze, Heze 274000, Shandong, China

Received:2022-02-09 Online:2022-10-25 Published:2022-10-26
Contact: LIU Yan

Abstract

Abstract:

Straight stem is one of the vital standards to judge cut peony. However, the factors affecting stem verticality of Paeonia lactiflora are not clear at present. To clarify the changes of cell wall components in stem characteristics of varieties with different straightness, six varieties were studied, and three levels of straightness were divided based on the angle between flower and plumb line. The process from budding to flower blooming was divided into S1 to S5 stages. Then, the key stage of stem bending in varieties with different straightness and dynamic changes of cell wall components in five stages were studied. The results showed that two cut peonies, Dongfang Shaonv and Yangfei Chuyu had relatively most straight stems. Chifen and other two cultivars bent along with stem development from S2 stage, and Qingwen bent rapidly from S4 stage. Similar trends were found in various cultivars that lignin content increased along with development and water-soluble pectin decreased from S2 stage. Correlation analysis showed that there was a significant positive correlation between stem straightness and lignin content at early development stage. The stem straightness was positively correlated with lignin content and negatively correlated with propectin content in the middle stage of development. The stem straightness was positively correlated with hemicellulose content and lignin content, and negatively correlated with water-soluble pectin content. The result would provide a theoretical basis for breeding and cultivation regulation of cut herbaceous peonies.

Key words: Paeonia lactiflora, stem straightness, cellulose, hemicellulose, lignin, protopectin, water-soluble pectin

CLC Number:

S682.12

HOU Jinghan, WAN Yingling, LIU Aiqing, HONG Aiying, LIU Yan. Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2220-2229.

Figures/Tables 9

Fig.1 Sampling time of Paeonia lactiflora A, Stage 1 (S1); B, Stage 2 (S2); C, Stage 3 (S3); D, Stage 4 (S4); E, Stage 5 (S5).

Fig.2 Measurement method of flower branch angle of Paeonia lactifloraα represented flower branch angle.

Table 1 Flower branch angle of various herbaceous peony cultivars at five developmental stages

品种名 Cultivar name	不同时期的花枝夹角 Flower branch angle at different stages/(°)					直立等级 Vertical grade
品种名 Cultivar name	S1	S2	S3	S4	S5	直立等级 Vertical grade
东方少女Dongfang Shaonv	3.38±0.73 Ba	5.78±0.65 Da	6.12±0.60 Ea	6.00±1.00 Da	5.95±0.62 Ea	Ⅰ
杨妃出浴Yangfei Chuyu	6.02±0.46 Ab	8.07±1.18 Cab	10.45±1.96 Da	7.30±1.74 Dab	10.53±0.84 Da	Ⅰ
迟粉Chifen	4.80±0.75 ABe	12.72±1.98 Bd	33.15±2.69 Ab	26.80±0.84 Cc	36.58±2.79 Ca	Ⅱ
种生粉Zhongshengfen	5.18±1.38 Ae	10.07±2.13 Cd	24.62±0.98 Bc	37.53±4.61 Bb	41.90±4.29 Ba	Ⅱ
奇花露霜Qihua Lushuang	4.52±0.90 ABd	16.48±2.97 Ac	13.82±2.04 Cc	36.97±3.82 Bb	56.38±4.66 Aa	Ⅲ
晴雯Qingwen	4.53±2.08 ABb	5.10±1.12 Db	5.83±1.08 Eb	54.05±5.15 Aa	54.23±5.12 Aa	Ⅲ

Fig.3 Cellulose content of Paeonia lactiflora with different vertical grades at five developmental stages Capital letters refered to the differences (P<0.05) between various varieties in the same stage, and lowercase letters refered to the differences (P<0.05) among different stages of a variety. The same as below.

Fig.4 Hemicellulose content of Paeonia lactiflora varieties with different vertical grades at five developmental stages

Fig.5 Lignin content of Paeonia lactiflora with different vertical grades at five developmental stages

Fig.6 Protopectin content of Paeonia lactiflora with different vertical grades at five developmental stages

Fig.7 Water-soluble pectin content of Paeonia lactiflora with different vertical grades at five developmental stages

Table 2 Correlation analysis between flower branch angle and cell wall component content of various herbaceous peony cultivars at five developmental stages

细胞壁组分 Cell wall components	发育时期Developmental stages
细胞壁组分 Cell wall components	S1	S2	S3	S4	S5
纤维素Cellulose	0.024	-0.405	-0.503	0.039	-0.090
半纤维素Hemicellulose	0.001	-0.210	-0.057	-0.604^**	0.024
木质素Lignin	-0.053	-0.761^**	-0.743^**	-0.419	-0.678^**
原果胶Protopectin	-0.379	-0.242	0.565^*	0.159	0.069
水溶性果胶Water-soluble pectin	-0.100	0.266	0.058	0.358	0.864^*

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Content changes of cell wall composition during stem development in different varieties of Paeonia lactiflora

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