Regulation effect of melatonin pretreatment on waterlogging tolerance in peach seedling

doi:10.3969/j.issn.1004-1524.2022.09.10

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (9): 1911-1924.DOI: 10.3969/j.issn.1004-1524.2022.09.10

• Horticultural Science • Previous Articles Next Articles

Regulation effect of melatonin pretreatment on waterlogging tolerance in peach seedling

GU Xianbin(), LU Linghong, SONG Genhua, XIAO Jinping, ZHANG Huiqin()

Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-11-24 Online:2022-09-25 Published:2022-09-30
Contact: ZHANG Huiqin

Abstract

Abstract:

In order to explore the regulation effect of melatonin on waterlogging tolerance in peach, peach seedlings were pretreated with 200 μmol·L^-1 melatonin and then subjected to waterlogging treatment. Physiological indexes and transcriptome sequencing were used to study the effects of melatonin on peach under waterlogging. The results showed that exogenous melatonin slowed chlorophyll degradation and protected cell membranes and roots of peach seedling. Melatonin activated microtubule morphogenesis, REDOX enzyme activity and protein-binding related genes, thus consolidated root skeleton, activated antioxidant enzyme activity and improved stress tolerance. Waterlogging induced abundant expression of ERF transcription factor family members to regulate stress response. All the VII group members of ERF family were significant differential expressed in the waterlogging response group, which could be the key member in hypoxia stress response reaction. In addition, ERF transcription factor activated the activity of key enzymes genes as ADH, ALD, GAPD in glycolysis pathway through HRPE or GCC-box cis-acting elements, which provided energy for plants under hypoxic stress. Prupe.8G264900, a member of ERF Ⅶ, might be a key gene in response to waterlogging in peach.

Key words: peach, melatonin, waterlogging, transcriptome

CLC Number:

S662.1

GU Xianbin, LU Linghong, SONG Genhua, XIAO Jinping, ZHANG Huiqin. Regulation effect of melatonin pretreatment on waterlogging tolerance in peach seedling[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1911-1924.

Figures/Tables 12

Table 1 Primers for qRT-PCR

基因序列号 Gene ID	基因名称 Gene name	上游引物 Forward primer (5'→3')	下游引物 Reverse primer (5'→3')	产物长度 Products lenth/bp
Prupe.1G234000	PpGAPDH	CTGCTTCCTTCAACATCAT	TGCCTTCTTCTCAATCCT	158
Prupe.1G305900	PpRAV	TTCAGATGGTTCGGCTATT	TCCTTGGCTTCTTGCTAA	183
Prupe.2G258200	PpERD15	GAGGAAGAGGAGAAGGATT	CGGACTCACTTTCATCAC	138
Prupe.2G272300	PpERF1	CGGATGCTTCAACAACAA	TACTGGTCTTCTGCCTTG	111
Prupe.3G236200	PpLBD41	GGATGAGTTGTAATGGATG	CCTCGTATAGCAATGATC	213
Prupe.6G230600	PpWRKY7	CAGGTGTCTTCAGTTGGA	AGAGTAATCATCAGGTGGAA	207
Prupe.8G018100	PpADH1	GGAGTGATGCTGAGTGAT	CAGACCTGTGGAGATACC	183
Prupe.8G264900	PpERF071	CCTCCAATTCCAATTCCAA	GCAATCGCATCCTTATTCA	226
Prupe.6G163400	PpACTIN	TCTTCCAACCATCACTCAT	GCCACAACCTTAATCTTCAT	211

Fig.1 Physiological indexes changes of peach after waterlogging treatment SPSS Statistics 17.0 software was used for significant difference analysis, one-way ANOVA was used for significance test, Duncan method was used for multiple comparison. Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05.

Fig.2 Number statistics of differentially expressed genes in groups

Fig.3 Enrichment of the GO items induced by melatonin A, GO enrichment analysis; B, Heat map of differential gene expression.

Fig.4 GO enrich and expression analysis of common differentially expressed genes A, Venn diagram between groups; B, GO enrichment analysis; C, Heat map of different expression gene.

Table 2 Different expressed transcription factors in the most enrich GO pathway

转录因子家族 TF family	数量 Number	转录因子家族 TF family	数量 Number
AP2/ERF	34	HD-ZIP	3
WRKY	23	NF-YA	3
bZIP	15	ARR-B	2
HSF	12	BES1	2
C2C2-GATA	4	NF-X1	1
MADS	4	SBP	1
AT-hook	3	Trihelix	1

Fig.5 Phylogenetic tree analysis of ERF family in peach and Arabidopsis thaliana

Table 3 Different expressed ERFs in each subfamily group

ERF家族 ERF family	数量 Number	百分比 Percentage/%	ERF家族 ERF family	数量 Number	百分比 Percentage/%
Ⅰ	1/6	16.7	Ⅶ	3/3	100
Ⅱ	1/8	12.5	Ⅷ	3/10	30
Ⅲ	5/22	22.7	Ⅸ	12/20	60
Ⅳ	2/7	28.6	Ⅹ	2/6	33.3
Ⅴ	0/12	0	Ⅺ-L	0/2	0
Ⅵ	0/6	0

Fig.6 FPKM number of different expressed ERF family members

Fig.7 KEGG pathway enrichment analysis and ERF transcription factor target gene prediction A, KEGG enrichment analysis; B, Expression heat map of glycolysis pathway genes; C, ERF target gene prediction.

Fig.8 qRT-PCR validation of relative expression levels of differential expressed genes

Fig.9 Hypothesized regulatory mechanism for melatonin in the waterlogging response pathway

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Regulation effect of melatonin pretreatment on waterlogging tolerance in peach seedling

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