Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides

doi:10.3969/j.issn.1004-1524.20230146

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 168-176.DOI: 10.3969/j.issn.1004-1524.20230146

• Plant Protection • Previous Articles Next Articles

Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides

XIA Zhijie¹(), ZHANG Lei¹, SONG Jianghua², FU Min¹, ZHANG Lixin¹^,^*()

1. Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
2. College of Horticulture, Anhui Agricultural University, Hefei 230036, China

Received:2023-02-11 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

To identify the pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii, and screen effective fungicides for the management of this disease, symptomatic samples were collected from the fields located in Shucheng County and Chaohu City, Anhui Province in 2021 and 2022. Pure cultures were obtained consistently using tissue separation method, and five representative isolates were selected for morphological observation and molecular identification. Partial fragments of internal transcribed spacer (ITS) and large subunit (LSU) regions, beta-tublin 2 (TUB2) genes of the isolates were amplified and sequenced. Pathogenicity assays were carried out to confirm Koch’s postulates. The pathogen was finally identified as Stagonosporopsis citrulli based on morphological characteristics and multi-loci sequence analysis. The indoor toxicity test results indicated that boscalid and prochloraz exhibited relatively higher inhibitory effects among seven fungicides, with both EC₅₀ ranging from 0.010 6 to 0.043 8 μg·mL^-1. The two fungicides could be preferentially utilized in rotation to control the disease caused by S. citrulli.

Key words: Cucumis melo, Trichosanthes kirilowii, gummy stem blight, fungicide

CLC Number:

S436.5

XIA Zhijie, ZHANG Lei, SONG Jianghua, FU Min, ZHANG Lixin. Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 168-176.

Figures/Tables 7

References 34

[1]	徐彦刚, 羊杏平, 李良俊. 瓜类作物抗蔓枯病研究进展[J]. 江苏农业科学, 2020, 48(3): 49-55.
	XU Y G, YANG X P, LI L J. Research progress on resistance of cucurbit crops to gummy stem blight[J]. Jiangsu Agricultural Sciences, 2020, 48(3): 49-55. (in Chinese with English abstract)
[2]	WEHNER T C, SHETTY N V. Screening the cucumber germplasm collection for resistance to gummy stem blight in north Carolina field tests[J]. HortScience, 2000, 35(6): 1132-1140.
[3]	WAKO T, SAKATA Y, SUGIYAMA M, et al. Identification of melon accessions resistant to gummy stem blight and genetic analysis of the resistance using an efficient technique for seedling test[J]. Acta Horticulturae, 2002(588): 161-164.
[4]	TSUTSUMI C Y, DA SILVA N. Screening of melon populations for resistance to Didymella bryoniae in greenhouse and plastic tunnel conditions[J]. Brazilian Archives of Biology and Technology, 2004, 47(2): 171-177.
[5]	WYSZOGRODZKA A J, WILLIAMS P H, PETERSON C E. Search for resistance to gummy stem blight (Didymella bryoniae) in cucumber (Cucumis sativus L.)[J]. Euphytica, 1986, 35(2): 603-613.
[6]	STEWART J E, TURNER A N, BREWER M T. Evolutionary history and variation in host range of three Stagonosporopsis species causing gummy stem blight of cucurbits[J]. Fungal Biology, 2015, 119(5): 370-382.
[7]	LI H X, BREWER M T. Spatial genetic structure and population dynamics of gummy stem blight fungi within and among watermelon fields in the southeastern United States[J]. Phytopathology, 2016, 106(8): 900-908.
[8]	秦健, 陈振东, 宋焕忠, 等. 广西苦瓜蔓枯病的病原分离与鉴定[J]. 植物病理学报, 2018, 48(2): 280-284.
	QIN J, CHEN Z D, SONG H Z, et al. Identification of causal organism of gummy stem blight of balsam pear in Guangxi[J]. Acta Phytopathologica Sinica, 2018, 48(2): 280-284. (in Chinese with English abstract)
[9]	陈悦, 孙东晗, 孙于淼, 等. 吉林省西瓜蔓枯病病原鉴定及室内药剂筛选[J]. 北方园艺, 2020(23): 16-23.
	CHEN Y, SUN D H, SUN Y M, et al. Pathogen identification and screening of the fungicides of Citrullus lanatus gummy stem blight in Jilin Province[J]. Northern Horticulture, 2020(23): 16-23. (in Chinese with English abstract)
[10]	胡锐, 邢彩云, 杨爱华, 等. 郑州市西瓜蔓枯病的发生及防治措施[J]. 中国瓜菜, 2010, 23(6): 46-47.
	HU R, XING C Y, YANG A H, et al. Occurrence and control measures of watermelon stem blight in Zhengzhou city[J]. China Cucurbits and Vegetables, 2010, 23(6): 46-47. (in Chinese with English abstract)
[11]	陈开端, 韩翠婷, 戴峥峰, 等. 浅谈西甜瓜蔓枯病和枯萎病的诊断与防治[J]. 中国蔬菜, 2019(8): 104-105.
	CHEN K D, HAN C T, DAI Z F, et al. Diagnosis and control of watermelon blight and Fusarium wilt[J]. China Vegetables, 2019(8): 104-105. (in Chinese with English abstract)
[12]	ZHANG Z G, ZHANG J Y, WANG Y C, et al. Molecular detection of Fusarium oxysporum f.sp. niveum and Mycosphaerella melonis in infected plant tissues and soil[J]. FEMS Microbiology Letters, 2005, 249(1): 39-47.
[13]	方中达. 植病研究方法[M]. 3版. 北京: 中国农业出版社, 1998: 122-142.
[14]	李英, 张永兵, JOSEPH N WOLUKAU, 等. 甜瓜蔓枯病菌子实体法分离及A型菌株产孢条件研究[J]. 果树学报, 2007, 24(1): 84-88.
	LI Y, ZHANG Y B, WOLUKAU J, et al. Fruit-body isolation of Didymella bryoniae and sporulation conditions of its A stain[J]. Journal of Fruit Science, 2007, 24(1): 84-88. (in Chinese with English abstract)
[15]	O’DONNELL K, CIGELNIK E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the FungusFusariumAre nonorthologous[J]. Molecular Phylogenetics and Evolution, 1997, 7(1): 103-116.
[16]	CHEN Q, JIANG J R, ZHANG G Z, et al. Resolving the Phoma enigma[J]. Studies in Mycology, 2015, 82: 137-217.
[17]	VALENZUELA-LOPEZ N, CANO-LIRA J F, GUARRO J, et al. Coelomycetous Dothideomycetes with emphasis on the families Cucurbitariaceae and Didymellaceae[J]. Studies in Mycology, 2018, 90: 1-69.
[18]	SEBLANI R, KEINATH A P, MUNKVOLD G. Gummy stem blight: one disease, three pathogens[J]. Molecular Plant Pathology, 2023, 24(8): 825-837.
[19]	LI H X, GOTTILLA T M, BREWER M T. Organization and evolution of mating-type genes in three Stagonosporopsis species causing gummy stem blight of cucurbits and leaf spot and dry rot of papaya[J]. Fungal Biology, 2017, 121(10): 849-857.
[20]	DE GRUYTER J, AVESKAMP M M, WOUDENBERG J H C, et al. Molecular phylogeny of Phoma and allied anamorph Genera: towards a reclassification of the Phoma complex[J]. Mycological Research, 2009, 113(4): 508-519.
[21]	DE GRUYTER J, WOUDENBERG J H C, AVESKAMP M M, et al. Systematic reappraisal of species in Phoma section Paraphoma, Pyrenochaeta and Pleurophoma[J]. Mycologia, 2010, 102(5): 1066-1081.
[22]	ZHANG Y, SCHOCH C L, FOURNIER J, et al. Multi-locus phylogeny of Pleosporales: a taxonomic, ecological and evolutionary re-evaluation[J]. Studies in Mycology, 2009, 64: 85-102.
[23]	HOU L W, GROENEWALD J Z, PFENNING L H, et al. The Phoma-like dilemma[J]. Studies in Mycology, 2020, 96: 309-396.
[24]	CHEN Q, HOU L W, DUAN W J, et al. Didymellaceae revisited[J]. Studies in Mycology, 2017, 87: 105-159.
[25]	AVESKAMP M M, DE GRUYTER J, WOUDENBERG J H C, et al. Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean Genera[J]. Studies in Mycology, 2010, 65: 1-60.
[26]	RENNBERGER G, KEINATH A P. Susceptibility of fourteen new cucurbit species to gummy stem blight caused by Stagonosporopsis citrulli under field conditions[J]. Plant Disease, 2018, 102(7): 1365-1375.
[27]	KEINATH A P. Differential susceptibility of nine cucurbit species to the foliar blight and crown canker phases of gummy stem blight[J]. Plant Disease, 2014, 98(2): 247-254.
[28]	HUANG C J, LAI Y R. First report of Stagonosporopsis citrulli causing gummy stem blight of watermelon in Taiwan[J]. Plant Disease, 2018, 101(2): 417.
[29]	谭蕊. 西南地区西瓜蔓枯病菌群体遗传结构研究[D]. 重庆: 西南大学, 2018.
	TAN R. Population genetic structure of gummy stem blight fungi in southwest China[D]. Chongqing: Southwest University, 2018. (in Chinese with English abstract)
[30]	李雨, 王少秋, 谭蕊, 等. 西瓜蔓枯病有效药剂筛选及药效评价[J]. 农药, 2016, 55(6): 460-462.
	LI Y, WANG S Q, TAN R, et al. Evaluation of fungicides for control of gummy stem blight caused by Didymella bryoniae[J]. Agrochemicals, 2016, 55(6): 460-462. (in Chinese with English abstract)
[31]	THOMAS A, LANGSTON D B JR, SANDERS H F, et al. Relationship between fungicide sensitivity and control of gummy stem blight of watermelon under field conditions[J]. Plant Disease, 2012, 96(12): 1780-1784.
[32]	KEINATH A P. Differential sensitivity to boscalid in conidia and ascospores of Didymella bryoniae and frequency of boscalid-insensitive isolates in south Carolina[J]. Plant Disease, 2012, 96(2): 228-234.
[33]	KEINATH A P. Soil amendment with cabbage residue and crop rotationto reduce gummy stem blight and increase growth and yield of watermelon[J]. Plant Disease, 1996, 80(5): 564.
[34]	KEINATH A. Polyoxin D and other biopesticides reduce gummy stem blight but not anthracnose on melon seedlings[J]. Plant Health Progress, 2016, 17: 177-181.

菌株编号 Isolate No.	GenBank登录号GenBank accession number
菌株编号 Isolate No.	LSU	ITS	TUB2
SC1-1	OP585646	OP585640	OP593318
SC2-1	OP585647	OP585641	OP593319
SC3-1	OP585648	OP585642	OP593320
CH1-1	OP585649	OP585643	OP593321
CH2-1	OP585650	OP585644	OP593322

菌株编号 Isolate No.	GenBank登录号GenBank accession number
菌株编号 Isolate No.	LSU	ITS	TUB2
SC1-1	OP585646	OP585640	OP593318
SC2-1	OP585647	OP585641	OP593319
SC3-1	OP585648	OP585642	OP593320
CH1-1	OP585649	OP585643	OP593321
CH2-1	OP585650	OP585644	OP593322

杀菌剂 Fungicide	SC1-1			SC2-1			SC3-1
杀菌剂 Fungicide	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²
啶酰菌胺boscalid	y=0.420 2x+5.571 0	0.043 8	0.956 2	y=0.880 6x+6.282 0	0.035 0	0.994 4	y=0.917 0x+6.392 0	0.035 0	0.973 7
咪鲜胺prochloraz	y=0.468 3x+5.906 6	0.011 6	0.978 4	y=0.880 6x+5.953 7	0.035 0	0.984 2	y=0.917 0x+5.918 9	0.030 3	0.971 8
苯醚甲环唑	y=0.802 5x+4.995 1	1.014 0	0.984 9	y=0.755 2x+5.046 6	0.867 5	0.985 3	y=0.733 0x+5.027 9	0.916 2	0.983 6
difenoconazole
吡唑醚菌酯	y=1.061 1x+4.520 7	2.829 6	0.985 2	y=1.111 1x+4.504 8	2.790 8	0.976 8	y=1.155 6x+4.556 0	2.422 1	0.982 9
pyraclostrobin
戊唑醇tebuconazole	y=0.533 9x+4.712 5	3.455 6	0.982 2	y=0.661 8x+4.682 6	3.017 2	0.989 9	y=0.553 2x+4.715 8	3.264 1	0.971 2
多菌灵carbendazim	y=0.983 3x+3.619 0	25.373 3	0.855 0	y=1.037 1x+3.625 7	21.137 0	0.872 1	y=1.007 3x+3.503 0	24.527 2	0.871 0
异菌脲iprodione	y=1.067 1x+4.736 0	1.767 5	0.915 8	y=0.949 8x+4.802 7	1.613 2	0.921 9	y=1.046 0x+5.040 1	0.915 4	0.941 1

杀菌剂 Fungicide	SC1-1			SC2-1			SC3-1
杀菌剂 Fungicide	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²	回归方程 Regression equation	EC₅₀/ (μg· mL^-1)	决定系数 R²
啶酰菌胺boscalid	y=0.420 2x+5.571 0	0.043 8	0.956 2	y=0.880 6x+6.282 0	0.035 0	0.994 4	y=0.917 0x+6.392 0	0.035 0	0.973 7
咪鲜胺prochloraz	y=0.468 3x+5.906 6	0.011 6	0.978 4	y=0.880 6x+5.953 7	0.035 0	0.984 2	y=0.917 0x+5.918 9	0.030 3	0.971 8
苯醚甲环唑	y=0.802 5x+4.995 1	1.014 0	0.984 9	y=0.755 2x+5.046 6	0.867 5	0.985 3	y=0.733 0x+5.027 9	0.916 2	0.983 6
difenoconazole
吡唑醚菌酯	y=1.061 1x+4.520 7	2.829 6	0.985 2	y=1.111 1x+4.504 8	2.790 8	0.976 8	y=1.155 6x+4.556 0	2.422 1	0.982 9
pyraclostrobin
戊唑醇tebuconazole	y=0.533 9x+4.712 5	3.455 6	0.982 2	y=0.661 8x+4.682 6	3.017 2	0.989 9	y=0.553 2x+4.715 8	3.264 1	0.971 2
多菌灵carbendazim	y=0.983 3x+3.619 0	25.373 3	0.855 0	y=1.037 1x+3.625 7	21.137 0	0.872 1	y=1.007 3x+3.503 0	24.527 2	0.871 0
异菌脲iprodione	y=1.067 1x+4.736 0	1.767 5	0.915 8	y=0.949 8x+4.802 7	1.613 2	0.921 9	y=1.046 0x+5.040 1	0.915 4	0.941 1

杀菌剂 Fungicide	CH1-1			CH2-1
杀菌剂 Fungicide	回归方程 Regression equation	EC₅₀/ (μg·mL^-1)	决定系数 R²	回归方程 Regression equation	EC₅₀/ (μg·mL^-1)	决定系数 R²
啶酰菌胺boscalid	y=0.951 9x+6.410 3	0.033 0	0.999 2	y=0.845 9x+6.692 7	0.029 6	0.996 7
咪鲜胺prochloraz	y=0.463 9x+5.911 6	0.010 8	0.977 5	y=0.467 1x+5.922 3	0.010 6	0.967 4
苯醚甲环唑difenoconazole	y=0.813 4x+4.975 7	1.071 1	0.966 4	y=0.784 8x+5.015 7	0.954 9	0.972 6
吡唑醚菌酯pyraclostrobin	y=0.876 0x+0.083 6	4.076 5	0.982 6	y=0.788 4x+3.983 1	4.222 7	0.980 7
戊唑醇tebuconazole	y=0.588 9x+4.723 0	2.953 6	0.962 9	y=0.590 3x+4.701 6	3.201 9	0.952 6
多菌灵carbendazim	y=1.539 6x+2.522 2	40.677 7	0.851 0	y=1.382 7x+2.845 4	36.165 2	0.809 5
异菌脲iprodione	y=1.357 1x+4.930 5	1.125 1	0.936 0	y=1.053 9x+4.824 2	1.468 2	0.941 7

Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 34

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Xiangyun, WANG Luyan, ZHANG Changpeng, LI Yanjie, ZHAO Xueping, JIANG Jinhua. Analysis of the registration of compound preparations of triazole fungicides and strobilurin fungicides [J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2121-2129.
[2]	LIU Na, FAN Qiaochu, ZHOU Jia, SONG Yajing, ZHANG Guwen, FENG Zhijuan, BO Yuanpeng, WANG Bin, GONG Yaming. Identification and control of anthracnose in vegetable soybean [J]. Acta Agriculturae Zhejiangensis, 2022, 34(12): 2682-2688.
[3]	LYU Lu, WU Shenggan, WANG Qiang, ZHAO Xueping, XU Mingfei. Primary risk assessment of several fungicides to typical vineyard terrestrial organisms [J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2512-2521.
[4]	XIONG Xue, ZHAO Lina, YANG Senlin, SAMIAH Arif, ZHANG Yidong. Genome-wide identification of CmCIPK family and its expression analysis under abiotic stress in melon [J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1625-1639.
[5]	WU Jiawei, YAO Zhangliang, HU Qiqi, ZHANG Jie, CHEN Yi, JIANG Jianrong, ZHOU Guoxin, WANG Xia. Fungicides and optimum time for control of pear rust in Tongxiang City, north Zhejiang, China [J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1668-1675.
[6]	SUN Caixia, OUYANG Zhizhou, LIU Yuhong, YU Guoguang. Residue dynamic and risk assessment of three fungicides in broccoli [J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1292-1299.
[7]	WANG Guorong, FENG Xiaoxiao, WU Huiming, CAO Tingting, LI Qian, ZHENG Yongli. Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides [J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 661-669.
[8]	XU Xuefen, NI Chunhui, LI Huixia, LI Huanyu, LI Wenhao, CHEN Yuan, HU Fangdi. Pathogen identification and indoor toxicity tests on root rot of Codonopsis pilosula [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 96-103.
[9]	ZHANG Lijun, ZHANG Hu, XU Mingfei, LIN Chunmian, WU Huizhen, XU Jie, QIAN Mingrong. Dynamics of 5 fungicides residue in grape brewing fermentation process [J]. , 2019, 31(1): 149-154.
[10]	XIE Yunye, ZENG Sijin, YUAN Yue, WANG Lianping, FANG Li, WANG Hanrong. Pathogen identification and susceptibility to fungicides on tea anthracnose in Xinchang of Zhejiang Province [J]. , 2018, 30(7): 1188-1193.
[11]	GE Jing, JIANG Jinhua, CAI Leiming. Study on toxicity of three kinds of triazole fungicides on zebrafish (Danio rerio) [J]. , 2018, 30(5): 744-755.
[12]	XIE Daoyan， YANG Zhenguo， ZHOU Chuntao， CHAI Jianping， DA Aisi， NI Jing， JIANG Xiujun， LUO Yanjie*. Safety evaluation of new fungicide fluxapyroxadpyraclostrobin to mulberry and silkworm [J]. , 2016, 28(4): 654-.
[13]	DUAN Hai-ming, YU Li, HUANG Wei-dong, YU Hai-bing. Screening of high efficient fungicide for the pathogen of tomato gray mold and research on novel mixed agent [J]. , 2016, 28(12): 2060-2067.
[14]	LI Xu\\|qing1， TIAN Zhong\\|ling2， ZHENG Ji\\|rong1， ZHENG Jing\\|wu2. Biological characteristics and fungicide screening of Stemphylium lycopersici causing tomato grey leaf spot [J]. , 2015, 27(11): 1953-.
[15]	PU Zhan\\|xyu;HUANG Zhen\\|dong;HU Xiu\\|rong;LI Hong\\|ye;*. In vitro toxicity and field control effect of six fungicides to Colletotrichum gloeosporioides causing citrus anthracnose [J]. , 2014, 26(1): 0-126.