Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage

doi:10.3969/j.issn.1004-1524.20240168

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (3): 521-529.DOI: 10.3969/j.issn.1004-1524.20240168

• Crop Science • Previous Articles Next Articles

Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage

SONG Xinlu¹(), FAN Shuhong², WU Guangqi¹, ZHAN Mengqi¹, HOU Qian¹, LI Mingyue¹, XU Yan¹^,^*()

1. School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, Shandong, China
2. Zaozhuang Agricultural and Rural Business Development Center, Zaozhuang 277099, Shandong, China

Received:2024-02-29 Online:2025-03-25 Published:2025-04-02

Abstract

Abstract:

Soil pollution often involves a combination of heavy metals and polycyclic aromatic hydrocarbon, pollutants enter the plant through the root system, seriously endangering the safety of crop production. In order to study the effect of compound pollution on crops, pot experiment was carried out to analyze the effects of single additions of phenanthrene and copper, as well as their combined addition, on rice physiological and biochemical properties and copper and phenanthrene accumulation in rice roots at tillering stage. The results showed that the electrolyte permeability of rice under combined pollution of phenanthrene and copper was 16.08 percentage points higher than phenanthrene treatment; the activities of peroxidase and catalase in rice root were significantly (P<0.05) decreased under the pollution treatment, but there was no significant difference between combined pollution treatment and single pollution treatment. The content of phenanthrene in rice roots and rhizosphere under combined pollution of copper and phenanthrene were 0.59 mg·kg^-1 and 6.51 mg·kg^-1, respectively, which were significantly (P<0.05) higher than those in phenanthrene treatment (0.01 mg·kg^-1 and 3.63 mg·kg^-1). The content of copper in the rice roots was ranged from 48.28 to 74.18 mg·kg^-1, there was no significant difference in copper content between the combined pollution treatment and the single pollution treatment. In conclusion, copper-phenanthrene combined pollution did not enhance the inhibition of antioxidant enzyme activities in rice, significantly increased the content of phenanthrene in rice root and rhizosphere soil, and aggravated phenanthrene pollution.

Key words: rice, transport and transfer, copper, phenanthrene, combined pollution

CLC Number:

S511
X173

SONG Xinlu, FAN Shuhong, WU Guangqi, ZHAN Mengqi, HOU Qian, LI Mingyue, XU Yan. Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage[J]. Acta Agriculturae Zhejiangensis, 2025, 37(3): 521-529.

Figures/Tables 5

Fig.1 Electrolyte leakage of rice roots

Fig.2 Activities of antioxidant enzymes in rice roots Data was detected based on fresh weight.

Fig.3 Microscope scanning of target area and copper distribution scanning by LA-ICP-MS for rice roots A, Microscope scanning of target area in longitudinal section of rice root; B, Microscope scanning of target area in transverse section of rice root; C, LA-ICP-MS copper distribution colormapof longitudinal section of rice root; D, LA-ICP-MS copper distribution colormap of transverse section of rice root.

Fig.4 The image of rice root tips under optical camera and the content distribution colormap of elements in rice roots Image resolution: 2 568 pixel×1 230 pixel; Size: 18 μm; Time:10 ms.

Fig.5 The contents of phenanthrene, total copper and available copper in different rhizocompartments

References 37

[1]	李婉怡, 於维维, 余琼阳, 等. 土壤重金属-有机物复合污染环境效应与修复技术研究进展[J]. 土壤, 2023, 55(3): 453-463.
	LI W Y, YU W W, YU Q Y, et al. Environmental effects and remediation technologies of heavy metal-organic pollutant co-contaminated soil: a review[J]. Soils, 2023, 55(3): 453-463. (in Chinese with English abstract)
[2]	TUKAJ Z, AKSMANN A. Toxic effects of anthraquinone and phenanthrenequinone upon Scenedesmus strains (green algae) at low and elevated concentration of CO₂[J]. Chemosphere, 2007, 66(3): 480-487.
[3]	MAHAR A, WANG P, ALI A, et al. Challenges and opportunities in the phytoremediation of heavy metals contaminated soils: a review[J]. Ecotoxicology and Environmental Safety, 2016, 126: 111-121.
[4]	袁源远, 刘瑛, 吴建富, 等. 稻田土壤铜含量对水稻籽粒灌浆特性与产量的影响[J]. 核农学报, 2023, 37(1): 188-195.
	YUAN Y Y, LIU Y, WU J F, et al. Effects of the content of Cu on grain filling properties and grain yield in paddy soils[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(1): 188-195. (in Chinese with English abstract)
[5]	沈国清, 陆贻通, 周培. 土壤环境中重金属和多环芳烃复合污染研究进展[J]. 上海交通大学学报(农业科学版), 2005, 23(1): 102-106.
	SHEN G Q, LU Y T, ZHOU P. Advances of research on combined pollution of heavy metals with polycyclic aromatic hydrocarbons(PAHs) in soil environment[J]. Journal of Shanghai Jiao Tong University(Agricultural Science), 2005, 23(1): 102-106. (in Chinese with English abstract)
[6]	王飞, 赵颖. 太原市污灌区农田土壤中多环芳烃污染特征及生态风险评价[J]. 生态环境学报, 2022, 31(1): 160-169.
	WANG F, ZHAO Y. Pollution characteristics and risk assessment of PAHs in agricultural soil from sewage irrigation area of Taiyuan City, Shanxi Province[J]. Ecology and Environmental Sciences, 2022, 31(1): 160-169. (in Chinese with English abstract)
[7]	THAVAMANI P, MEGHARAJ M, NAIDU R. Multivariate analysis of mixed contaminants (PAHs and heavy metals) at manufactured gas plant site soils[J]. Environmental Monitoring and Assessment, 2012, 184(6): 3875-3885.
[8]	ZHANG P, CHEN Y G. Polycyclic aromatic hydrocarbons contamination in surface soil of China: a review[J]. Science of the Total Environment, 2017, 605/606: 1011-1020.
[9]	林义章, 徐磊. 铜污染对高等植物的生理毒害作用研究[J]. 中国生态农业学报, 2007, 15(1): 201-204.
	LIN Y Z, XU L. Physiological toxicity of copper pollution to higher plant[J]. Chinese Journal of Eco-Agriculture, 2007, 15(1): 201-204. (in Chinese with English abstract)
[10]	李大辉, 施国新. Cd²⁺或Hg²⁺水污染对菱体细胞的细胞核及叶绿体超微结构的影响[J]. 植物资源与环境, 1999(2):43-48.
	LI D H, SHI G X. Effects of Cd²⁺ or Hg²⁺ water pollution on the ultrastructure of nuclei and chloroplasts in somatic cells of Trapa bicornis Osbeck[J]. Journal of Plant Resources and Environment, 1999(2):43-48. (in Chinese with English abstract)
[11]	吴嘉煜, 米楠. 重金属对植物抗氧化酶影响研究进展[J]. 浙江农业科学, 2022, 63(6): 1177-1181.
	WU J Y, MI N. Effect of heavy metals on antioxidant enzymes in plants[J]. Journal of Zhejiang Agricultural Sciences, 2022, 63(6): 1177-1181. (in Chinese)
[12]	蔡顺香, 何盈, 王煌平, 等. 芘对小白菜幼苗生长和一些生理生化指标的影响[J]. 植物生理学通讯, 2008, 44(4): 643-646.
	CAI S X, HE Y, WANG H P, et al. Effects of pyrene on growth and several physiological and biochemical indexes of Chinese cabbage seedlings[J]. Plant Physiology Communications, 2008, 44(4): 643-646. (in Chinese with English abstract)
[13]	陈艳楠, 钱雨欣, 李志美, 等. 中国土壤铜的区域分级基准建立及生态风险评估初探[J]. 环境科学学报, 2023, 43(3): 448-458.
	CHEN Y N, QIAN Y X, LI Z M, et al. Preliminary study on regional grading benchmarking and ecological risk assessment of copper in Chinese soils[J]. Acta Scientiae Circumstantiae, 2023, 43(3): 448-458. (in Chinese with English abstract)
[14]	葛峰, 张转霞, 扶恒, 等. 我国有机污染场地现状分析及展望[J]. 土壤, 2021, 53(6): 1132-1141.
	GE F, ZHANG Z X, FU H, et al. Distribution of organic contaminated sites in China: statu quo and prospect[J]. Soils, 2021, 53(6): 1132-1141. (in Chinese with English abstract)
[15]	生态环境部. 土壤环境质量标准: GB 15618—1995[S]. 北京: 中国标准出版社, 1995.
[16]	CAI Q Y, MO C H, WU Q T, et al. The status of soil contamination by semivolatile organic chemicals (SVOCs) in China: a review[J]. The Science of the Total Environment, 2008, 389(2/3): 209-224.
[17]	DIONISIO-SESE M L, TOBITA S. Antioxidant responses of rice seedlings to salinity stress[J]. Plant Science, 1998, 135(1): 1-9.
[18]	WANI A S, AHMAD A, HAYAT S, et al. Epibrassinolide and proline alleviate the photosynthetic and yield inhibition under salt stress by acting on antioxidant system in mustard[J]. Plant Physiology and Biochemistry, 2019, 135: 385-394.
[19]	CHEN J, XIA X H, ZHANG Z R, et al. The combination of warming and copper decreased the uptake of polycyclic aromatic hydrocarbons by spinach and their associated cancer risk[J]. Science of the Total Environment, 2020, 727: 138732.
[20]	谷巍, 施国新, 巢建国, 等. 汞、镉、铜污染对鱼草细胞膜系统的毒害作用[J]. 应用生态学报, 2008, 19(5): 1138-1143.
	GU W, SHI G X, CHAO J G, et al. Toxic effects of Hg²⁺, Cd²⁺ and Cu²⁺ on cell membrane system of Cabomba caroliniana A. Gray[J]. Chinese Journal of Applied Ecology, 2008, 19(5): 1138-1143. (in Chinese with English abstract)
[21]	杨国远, 万凌琳, 雷学青, 等. 重金属铅、铬胁迫对斜生栅藻的生长、光合性能及抗氧化系统的影响[J]. 环境科学学报, 2014, 34(6): 1606-1614.
	YANG G Y, WAN L L, LEI X Q, et al. Effects of lead and chromium on the growth, photosynthetic performance, and antioxidant activity of Scenedesmus obliquus[J]. Acta Scientiae Circumstantiae, 2014, 34(6): 1606-1614. (in Chinese with English abstract)
[22]	许晓毅, 崔佳豪, 白净, 等. 两株多环芳烃降解菌协同对菲-镉污染的去除特性[J]. 微生物学报, 2023, 63(1): 283-296.
	XU X Y, CUI J H, BAI J, et al. Synergistic removal of phenanthrene and cadmium by two polycyclic aromatic hydrocarbon-degrading bacteria[J]. Acta Microbiologica Sinica, 2023, 63(1): 283-296. (in Chinese with English abstract)
[23]	铁柏清, 孙健, 钱湛, 等. 重金属复合污染对灯心草的生态毒性效应及重金属积累特性的影响[J]. 农业环境科学学报, 2006, 25(3): 629-636. (in Chinese with English abstract)
	TIE B Q, SUN J, QIAN Z, et al. The eco-toxicological effect of Cu, Cd, Pb, Zn and As compound pollution on Juncus effuses and its accumulation character of heavy metals[J]. Journal of Agro-Environment Science, 2006, 25(3): 629-636. (in Chinese with English abstract)
[24]	覃勇荣, 汤丰瑜, 严海杰, 等. 重金属胁迫对任豆种子萌发及幼苗抗氧化酶活性的影响[J]. 种子, 2017, 36(10): 31-36.
	QIN Y R, TANG F Y, YAN H J, et al. Effects of heavy metal stress on seed germination and seedlings antioxidant enzyme activity of Zenia insigni[J]. Seed, 2017, 36(10): 31-36. (in Chinese with English abstract)
[25]	梁勇生, 黄杏, 龙明华, 等. 多环芳烃胁迫对菜心生长及生理特性的影响[J]. 华南农业大学学报, 2018, 39(6): 54-60.
	LIANG Y S, HUANG X, LONG M H, et al. Effects of polycyclic aromatic hydrocarbons stress on growth and physiological characteristics of Brassica parachinensis[J]. Journal of South China Agricultural University, 2018, 39(6): 54-60. (in Chinese with English abstract)
[26]	汪攀, 陈奶莲, 邹显花, 等. 植物根系解剖结构对逆境胁迫响应的研究进展[J]. 生态学杂志, 2015, 34(2): 550-556.
	WANG P, CHEN N L, ZOU X H, et al. Research progress on adaptive responses of anatomical structure of plant roots to stress[J]. Chinese Journal of Ecology, 2015, 34(2): 550-556. (in Chinese with English abstract)
[27]	郦逸根, 薛生国, 吴小勇. 重金属在土壤: 水稻系统中的迁移转化规律研究[J]. 中国地质, 2004, 31(S1): 87-92.
	LI Y G, XUE S G, WU X Y. Transport and transformation of heavy metals in the soil-paddy plant system[J]. Geology in China, 2004, 31(S1): 87-92. (in Chinese)
[28]	SONG J, YANG Y Q, ZHU S H, et al. Spatial distribution and speciation of copper in root tips of cucumber revealed by μ-XRF and μ-XANES[J]. Biologia Plantarum, 2013, 57(3): 581-586.
[29]	董亚玲, 刘斌美, 陈慧茹, 等. 磷营养元素与水稻幼苗镉吸收关系研究[J]. 广东农业科学, 2014, 41(13): 6-8.
	DONG Y L, LIU B M, CHEN H R, et al. Relationship between phosphorus and cadmium absorption of rice seedlings[J]. Guangdong Agricultural Sciences, 2014, 41(13): 6-8. (in Chinese with English abstract)
[30]	傅友强, 于智卫, 蔡昆争, 等. 水稻根表铁膜形成机制及其生态环境效应[J]. 植物营养与肥料学报, 2010, 16(6): 1527-1534.
	FU Y Q, YU Z W, CAI K Z, et al. Mechanisms of iron plaque formation on root surface of rice plants and their ecological and environmental effects: a review[J]. Plant Nutrition and Fertilizer Science, 2010, 16(6): 1527-1534. (in Chinese with English abstract)
[31]	赵红, 许俊道, 徐卫红, 等. 硅营养对铜毒害水稻种子萌发及幼苗生长的缓解效应[J]. 上饶师范学院学报, 2009, 29(6): 75-79.
	ZHAO H, XU J D, XU W H, et al. Mitigative effect of silicon nutrition on copper toxicity in seed germination and seedling growth of rice[J]. Journal of Shangrao Normal University, 2009, 29(6): 75-79. (in Chinese with English abstract)
[32]	张昕怡, 田卓炎, 张成, 等. 植物修复多环芳烃污染土壤的根际效应机制研究进展[J]. 土壤通报, 2021, 52(5): 1251-1260.
	ZHANG X Y, TIAN Z Y, ZHANG C, et al. The mechanism of rhizosphere effect on phytoremediation of polycyclic aromatic hydrocarbons in soil: a review[J]. Chinese Journal of Soil Science, 2021, 52(5): 1251-1260. (in Chinese with English abstract)
[33]	CHEEMA S A, KHAN M I, TANG X J, et al. Enhancement of phenanthrene and pyrene degradation in rhizosphere of tall fescue (Festuca arundinacea)[J]. Journal of Hazardous Materials, 2009, 166(2/3): 1226-1231.
[34]	陈建, 夏星辉, 张真瑞, 等. 增温和锌复合作用对菠菜富集多环芳烃的影响[J]. 环境科学学报, 2020, 40(12): 4531-4539.
	CHEN J, XIA X H, ZHANG Z R, et al. The effects of elevated temperature and zinc combination on the accumulation of polycyclic aromatic hydrocarbons in spinach[J]. Acta Scientiae Circumstantiae, 2020, 40(12): 4531-4539. (in Chinese with English abstract)
[35]	LU M, XU K, CHEN J. Effect of pyrene and cadmium on microbial activity and community structure in soil[J]. Chemosphere, 2013, 91(4): 491-497.
[36]	VAN DER ENT A, ECHEVARRIA G, TIBBETT M. Delimiting soil chemistry thresholds for nickel hyperaccumulator plants in Sabah (Malaysia)[J]. Chemoecology, 2016, 26(2): 67-82.
[37]	MALISZEWSKA-KORDYBACH B, SMRECZAK B. Habitat function of agricultural soils as affected by heavy metals and polycyclic aromatic hydrocarbons contamination[J]. Environment International, 2003, 28(8): 719-728.

Effects of combined copper-phenanthrene pollution on physiological characteristics and pollutant accumulation of rice roots at tillering stage

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