Effect of grafting on cold resistance of potato seedlings under low temperature stress

doi:10.3969/j.issn.1004-1524.20240718

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1615-1623.DOI: 10.3969/j.issn.1004-1524.20240718

• Crop Science • Previous Articles Next Articles

Effect of grafting on cold resistance of potato seedlings under low temperature stress

LIU Guomin(), ZHENG Xu, LIAO Yujiao, QIN Yexin, QIN Weizhi()

Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Received:2024-08-06 Online:2025-08-25 Published:2025-09-03
Contact: QIN Weizhi

Abstract

Abstract:

To investigate the effects of grafting on different potato materials under lowtemperature stress, cold-resistant cultivar Guinongshu 1 (G1) and cold-sensitive cultivar Longshu 9 (L9) were reciprocally grafted, yielding two graft combinations (L9G1 and G1L9). G1, G1L9, L9G1, and L9 were subjected to 4 ℃ and 0 ℃ treatments in a light incubator, with 25 ℃ as the control. Antioxidant enzyme activities, membrane lipid peroxidation indices, and osmotic adjustment substance contents in potato leaves were measured. Cold resistance coefficients of relevant indicators were calculated and analyzed using correlation analysis, principal component analysis (PCA), membership function values, and weighting methods to comprehensively evaluate cold resistance. Results showed that, soluble sugar (SS) content was significantly (p<0.05) positively correlated with the cold resistance coefficient of catalase (CAT) activity, while relative electrical conductivity (REC) was significantly negatively correlated with the cold resistance coefficients of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Nine individual indicators were transformed into two comprehensive indicators by PCA. SOD activity, MDA content, soluble protein (SP) content, REC, and CAT activity could be served as key indicators to evaluate potato cold resistance. The comprehensive cold resistance ability of these four materials followed the order of G1>L9G1>G1L9>L9. It was concluded that the cold-resistant potato cultivars enhanced the cold resistance of graft combinations whether used as rootstock or scion, with rootstock exerting a greater influence.

Key words: potato, grafting, cold resistance, principal component analysis, membership function analysis, comprehensive cold resistance

CLC Number:

S532

LIU Guomin, ZHENG Xu, LIAO Yujiao, QIN Yexin, QIN Weizhi. Effect of grafting on cold resistance of potato seedlings under low temperature stress[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1615-1623.

Figures/Tables 7

References 41

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材料 Material	处理温度 Treatment temperature/℃	MDA/ (nmol·g^-1)	Pro/ (μg·g^-1)	SS/ (mg·g^-1)	SP/ (mg·g^-1)	H₂O₂/ (μmol·g^-1)	REC/%
L9	25	23.12 c	66.91 b	8.53 c	4.11 b	0.93 b	68.92 a
	4	36.86 b	150.27 a	26.17 a	4.69 a	1.37 a	60.38 a
	0	46.97 a	148.50 a	9.95 b	4.22 b	1.52 a	61.19 a
G1	25	12.49 b	46.22 c	4.20 b	3.47 b	1.02 b	72.82 b
	4	23.54 a	89.56 b	12.34 a	4.14 a	1.22 a	60.73 c
	0	24.27 a	115.42 a	12.59 a	2.87 c	0.99 b	82.62 a
G1L9	25	21.17 a	52.43 c	6.75 c	2.40 c	1.09 b	40.92 c
	4	24.24 a	187.67 a	15.46 a	4.32 a	1.26 a	58.22 b
	0	24.88 a	121.19 b	12.46 b	3.83 b	0.97 b	87.48 a
L9G1	25	22.03 c	56.05 c	8.91 c	2.70 b	2.52 a	67.00 a
	4	39.72 b	161.27 b	19.33 a	3.10 a	1.58 b	59.24 a
	0	49.90 a	207.43 a	16.60 b	3.19 a	1.14 c	63.94 a

材料 Material	处理温度 Treatment temperature/℃	MDA/ (nmol·g^-1)	Pro/ (μg·g^-1)	SS/ (mg·g^-1)	SP/ (mg·g^-1)	H₂O₂/ (μmol·g^-1)	REC/%
L9	25	23.12 c	66.91 b	8.53 c	4.11 b	0.93 b	68.92 a
	4	36.86 b	150.27 a	26.17 a	4.69 a	1.37 a	60.38 a
	0	46.97 a	148.50 a	9.95 b	4.22 b	1.52 a	61.19 a
G1	25	12.49 b	46.22 c	4.20 b	3.47 b	1.02 b	72.82 b
	4	23.54 a	89.56 b	12.34 a	4.14 a	1.22 a	60.73 c
	0	24.27 a	115.42 a	12.59 a	2.87 c	0.99 b	82.62 a
G1L9	25	21.17 a	52.43 c	6.75 c	2.40 c	1.09 b	40.92 c
	4	24.24 a	187.67 a	15.46 a	4.32 a	1.26 a	58.22 b
	0	24.88 a	121.19 b	12.46 b	3.83 b	0.97 b	87.48 a
L9G1	25	22.03 c	56.05 c	8.91 c	2.70 b	2.52 a	67.00 a
	4	39.72 b	161.27 b	19.33 a	3.10 a	1.58 b	59.24 a
	0	49.90 a	207.43 a	16.60 b	3.19 a	1.14 c	63.94 a

材料 Material	处理温度 Treatment temperature/℃	SOD/ (U·g^-1)	CAT/ (μmoL· min^-1·g^-1)	POD/ (μmoL· min^-1·g^-1)
L9	25	1 161.20 c	733.54 a	2 255.14 a
	4	2 519.28 a	739.69 a	1 968.43 b
	0	1 958.00 b	270.90 b	2 226.63 a
G1	25	1 055.04 b	184.74 c	734.48 c
	4	1 668.84 a	680.60 a	1 273.08 b
	0	1 750.65 a	298.52 b	1 852.94 a
G1L9	25	1 424.74 a	240.76 c	684.82 b
	4	1 349.38 a	349.45 a	463.14 c
	0	1 238.98 a	272.51 b	934.69 a
L9G1	25	1 215.97 c	229.82 b	696.45 c
	4	1 758.16 b	264.91 a	2 462.18 a
	0	1 977.45 a	229.36 b	1 564.87 b

材料 Material	处理温度 Treatment temperature/℃	SOD/ (U·g^-1)	CAT/ (μmoL· min^-1·g^-1)	POD/ (μmoL· min^-1·g^-1)
L9	25	1 161.20 c	733.54 a	2 255.14 a
	4	2 519.28 a	739.69 a	1 968.43 b
	0	1 958.00 b	270.90 b	2 226.63 a
G1	25	1 055.04 b	184.74 c	734.48 c
	4	1 668.84 a	680.60 a	1 273.08 b
	0	1 750.65 a	298.52 b	1 852.94 a
G1L9	25	1 424.74 a	240.76 c	684.82 b
	4	1 349.38 a	349.45 a	463.14 c
	0	1 238.98 a	272.51 b	934.69 a
L9G1	25	1 215.97 c	229.82 b	696.45 c
	4	1 758.16 b	264.91 a	2 462.18 a
	0	1 977.45 a	229.36 b	1 564.87 b

材料 Material	SOD	CAT	POD	MDA	Pro	SS	SP	H₂O₂	REC
L9	168.62	36.93	98.74	203.13	221.93	116.62	102.82	164.24	88.78
G1	165.93	161.58	252.28	194.26	249.70	299.65	82.77	97.38	113.46
G1L9	86.96	113.19	136.49	117.56	231.16	184.69	159.26	88.84	213.77
L9G1	162.62	99.80	224.69	226.54	370.05	186.33	117.94	45.00	95.43

Effect of grafting on cold resistance of potato seedlings under low temperature stress

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指标 Index	SOD	CAT	POD	MDA	Pro	SS	SP	H₂O₂
CAT	-0.162
POD	0.340	0.809
MDA	0.942	-0.203	0.389
Pro	0.302	0.120	0.574	0.586
SS	0.087	0.961^*	0.851	-0.011	0.076
SP	-0.907	-0.128	-0.445	-0.737	-0.027	-0.394
H₂O₂	0.197	-0.571	-0.691	-0.056	-0.797	-0.420	-0.282
REC	-0.984^*	0.303	-0.242	-0.968^*	-0.367	0.072	0.821	-0.192

主成分	特征值	贡献率	累计贡献率
Principal component	Eigenvalue	Contribution rate/%	Cumulative contribution rate/%
1	4.152	46.128	46.128
2	3.263	36.252	82.380
3	1.586	17.620	100.000

指标 Index	主成分载荷矩阵 Principal component load matrix		特征向量 Eigenvector
指标 Index	PC1	PC2	PC1	PC2
SOD	0.896	-0.432	0.440	-0.240
CAT	0.218	0.909	0.110	0.500
POD	0.712	0.701	0.350	0.390
MDA	0.908	-0.352	0.450	-0.190
Pro	0.567	0.295	0.280	0.160
SS	0.409	0.765	0.200	0.420
SP	-0.838	0.249	-0.410	0.140
H₂O₂	-0.227	-0.794	-0.110	-0.440
REC	-0.853	0.519	-0.420	0.290

材料 Material	综合得分 Comprehensive score		隶属函数值 Membership function value		综合抗寒能力 Comprehensive cold resistance ability	排序 Ranking
材料 Material	F₁	F₂	μ₁	μ₂	综合抗寒能力 Comprehensive cold resistance ability	排序 Ranking
L9	-0.205	-2.664	0.603	0	0.338	4
G1	1.511	1.141	1.000	1.000	1.000	1
G1L9	-2.815	1.061	0	0.979	0.431	3
L9G1	1.509	0.463	0.999	0.822	0.921	2
权重Weight	—	—	0.560	0.440	—	—

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