Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province, China

doi:10.3969/j.issn.1004-1524.20250285

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (8): 1517-1528.DOI: 10.3969/j.issn.1004-1524.20250285

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Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province, China

GUO Wei¹(), MA Xiansen¹, NIE Hongyan¹, SONG Zhihao¹, ZHOU Yi¹, CHEN Guangxun¹, CHEN Zhengwu²^,^*()

¹ Tea and Fruit Research Institute, Bijie Academy of Agricultural Sciences, Bijie 551700, Guizhou, China
² Guizhou Tea Research Institute, Guiyang 550006, China

Received:2025-04-07 Online:2026-08-25 Published:2026-09-14

Abstract

Abstract:

To explore the quality characteristics of biochemical components in natural hybrid progenies derived from several local landraces and tea cultivar Huangjinya, and to supply theoretical references for future novel tea cultivar breeding, high-performance liquid chromatography (HPLC) was adopted to quantify 28 biochemical components across 35 tea accessions conserved in the germplasm nursery of Guizhou Tea Research Institute. Multiple statistical approaches including genetic diversity analysis, correlation analysis, principal component analysis (PCA) and cluster analysis were integrated to conduct a comprehensive evaluation of all tested accessions. The results revealed that the average coefficient of variation (CV) of the 28 biochemical components reached 43.04%. Cysteine (Cys) content exhibited the maximum CV of 117.40%, while caffeine (CAF) content showed the minimum CV of 14.55%. The mean value of the genetic diversity index was 1.81; phenylalanine (Phe) content possessed the highest index value of 2.09, and gallocatechin gallate (GCG) content had the lowest value of 0.66. Overall, amino acid components presented relatively high genetic diversity indexes. Seven principal components were extracted via PCA, accounting for a cumulative variance contribution rate of 78.451%. Total catechins (TC), arginine (Arg), epigallocatechin gallate (EGCG), glutamic acid (Glu), epicatechin gallate (ECG) and theanine (The) contents were identified as the core evaluation indicators. All tested accessions were divided into 4 groups by cluster analysis. Group Ⅳ, characterized by high amino acid contents as well as low alkaloid and catechin levels, obtained the optimal comprehensive score and possessed excellent suitability for manufacturing premium green tea. Based on the integrated evaluation results, 9 elite accessions represented by GT-AS-70 and GT-AS-83 were screened for developing green tea-specific cultivars.

Key words: tea plant resource, biochemical component, genetic diversity, principal component analysis, cluster analysis

CLC Number:

S571.1

GUO Wei, MA Xiansen, NIE Hongyan, SONG Zhihao, ZHOU Yi, CHEN Guangxun, CHEN Zhengwu. Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province, China[J]. Acta Agriculturae Zhejiangensis, 2026, 38(8): 1517-1528.

Figures/Tables 7

References 30

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生化成分 Biochemical component	含量/(mg·g^-1) Content/(mg·g^-1)	CV/%	H'
TB	9.34±2.64	28.32	1.74
TP	0.13±0.06	46.58	1.90
CAF	33.78±4.91	14.55	1.91
GA	0.51±0.15	28.32	1.88
EGC	14.76±3.57	24.18	2.00
C	0.49±0.11	21.34	1.83
EC	6.82±1.81	26.54	2.00
EGCG	56.68±13.01	22.95	1.78
GCG	7.80±3.95	50.66	0.66
ECG	18.79±6.77	36.04	1.77
TC	105.35±19.33	18.35	1.91
THE	28.87±5.96	20.65	1.76
ASP	2.48±0.82	33.12	1.79
SER	1.18±0.28	23.67	1.96
GLU	5.31±1.71	32.19	1.66
GLY	0.09±0.04	48.34	1.84
HIS	4.87±2.66	54.71	1.82
ARG	6.00±3.00	50.02	1.98
THR	0.15±0.11	69.19	1.88
ALA	0.26±0.16	63.44	1.90
PRO	1.18±0.46	38.73	1.91
CYS	0.01±0.01	117.40	1.60
TYR	0.06±0.05	79.75	1.73
VAL	3.41±1.27	37.27	2.01
MET	0.16±0.11	66.27	1.84
LYS	0.08±0.06	81.58	1.80
LEU	0.14±0.06	44.05	1.63
PHE	0.24±0.06	26.90	2.09

生化成分 Biochemical component	含量/(mg·g^-1) Content/(mg·g^-1)	CV/%	H'
TB	9.34±2.64	28.32	1.74
TP	0.13±0.06	46.58	1.90
CAF	33.78±4.91	14.55	1.91
GA	0.51±0.15	28.32	1.88
EGC	14.76±3.57	24.18	2.00
C	0.49±0.11	21.34	1.83
EC	6.82±1.81	26.54	2.00
EGCG	56.68±13.01	22.95	1.78
GCG	7.80±3.95	50.66	0.66
ECG	18.79±6.77	36.04	1.77
TC	105.35±19.33	18.35	1.91
THE	28.87±5.96	20.65	1.76
ASP	2.48±0.82	33.12	1.79
SER	1.18±0.28	23.67	1.96
GLU	5.31±1.71	32.19	1.66
GLY	0.09±0.04	48.34	1.84
HIS	4.87±2.66	54.71	1.82
ARG	6.00±3.00	50.02	1.98
THR	0.15±0.11	69.19	1.88
ALA	0.26±0.16	63.44	1.90
PRO	1.18±0.46	38.73	1.91
CYS	0.01±0.01	117.40	1.60
TYR	0.06±0.05	79.75	1.73
VAL	3.41±1.27	37.27	2.01
MET	0.16±0.11	66.27	1.84
LYS	0.08±0.06	81.58	1.80
LEU	0.14±0.06	44.05	1.63
PHE	0.24±0.06	26.90	2.09

主成分 Principal component	特征值 Eigenvalue	方差贡献率/% Variance contribution rate/%	累计方差贡献率/% Cumulative variance contribution rate/%
1	8.429	30.101	30.101
2	4.218	15.066	45.167
3	2.611	9.325	54.492
4	2.318	8.280	62.772
5	1.921	6.862	69.634
6	1.428	5.099	74.733
7	1.041	3.717	78.451

主成分 Principal component	特征值 Eigenvalue	方差贡献率/% Variance contribution rate/%	累计方差贡献率/% Cumulative variance contribution rate/%
1	8.429	30.101	30.101
2	4.218	15.066	45.167
3	2.611	9.325	54.492
4	2.318	8.280	62.772
5	1.921	6.862	69.634
6	1.428	5.099	74.733
7	1.041	3.717	78.451

生化成分 Biochemical component	PC1		PC2		PC3		PC4		PC5		PC6		PC7
生化成分 Biochemical component	Lo	SC	Lo	SC	Lo	SC	Lo	SC	Lo	SC	Lo	SC	Lo	SC
TB	-0.571	-0.068	0.466	0.110	-0.276	-0.106	-0.061	-0.026	0.270	0.140	-0.039	-0.027	-0.022	-0.021
TP	-0.627	-0.074	0.223	0.053	-0.098	-0.037	0.119	0.051	0.104	0.054	-0.009	-0.006	-0.094	-0.090
CAF	-0.639	-0.076	0.351	0.083	0.426	0.163	-0.022	-0.009	0.030	0.016	0.074	0.052	-0.081	-0.078
GA	-0.263	-0.031	0.576	0.136	0.035	0.013	-0.211	-0.091	0.107	0.056	0.047	0.033	0.316	0.304
EGC	-0.194	-0.023	-0.564	-0.134	0.649	0.249	0.011	0.005	0.169	0.088	-0.017	-0.012	-0.110	-0.105
C	-0.543	-0.064	0.316	0.075	0.005	0.002	0.276	0.119	-0.440	-0.229	-0.206	-0.145	0.247	0.238
EC	-0.027	-0.003	-0.127	-0.030	0.481	0.184	0.643	0.277	0.167	0.087	-0.135	-0.094	0.317	0.305
EGCG	-0.813	-0.096	0.155	0.037	0.154	0.059	0.044	0.019	-0.039	-0.020	0.229	0.160	-0.230	-0.221
GCG	-0.198	-0.024	0.177	0.042	-0.495	-0.189	-0.135	-0.058	0.574	0.299	0.318	0.222	-0.105	-0.101
ECG	-0.719	-0.085	0.550	0.130	-0.033	-0.013	0.256	0.110	0.090	0.047	-0.184	-0.129	0.107	0.103
TC	-0.881	-0.105	0.219	0.052	0.156	0.060	0.156	0.067	0.167	0.087	0.138	0.097	-0.128	-0.123
THE	0.689	0.082	-0.027	-0.006	-0.127	-0.048	0.363	0.157	0.040	0.021	-0.299	-0.209	-0.067	-0.065
ASP	0.513	0.061	0.115	0.027	-0.037	-0.014	0.486	0.210	-0.186	-0.097	0.419	0.294	0.237	0.227
SER	0.610	0.072	0.676	0.160	-0.002	-0.001	0.089	0.039	-0.011	-0.006	-0.165	-0.115	-0.014	-0.014
GLU	0.756	0.090	0.145	0.034	-0.065	-0.025	0.350	0.151	-0.242	-0.126	0.171	0.120	-0.013	-0.013
GLY	0.146	0.017	-0.278	-0.066	0.610	0.233	0.254	0.109	0.486	0.253	0.273	0.191	0.055	0.052
HIS	0.625	0.074	0.198	0.047	-0.260	-0.100	0.440	0.190	-0.153	-0.080	0.263	0.184	-0.281	-0.270
ARG	0.848	0.101	0.172	0.041	-0.034	-0.013	-0.042	-0.018	0.266	0.138	0.089	0.062	-0.120	-0.116
THR	0.503	0.060	0.733	0.174	-0.057	-0.022	-0.030	-0.013	0.035	0.018	0.054	0.038	-0.033	-0.031
ALA	0.543	0.064	0.536	0.127	-0.033	-0.013	0.276	0.119	0.278	0.145	-0.172	-0.120	-0.184	-0.177
PRO	-0.398	-0.047	0.305	0.072	0.300	0.115	0.454	0.196	-0.269	-0.140	0.458	0.321	-0.072	-0.070
CYS	0.303	0.036	-0.028	-0.007	-0.232	-0.089	-0.030	-0.013	0.563	0.293	0.246	0.172	0.572	0.550
TYR	0.343	0.041	0.519	0.123	0.497	0.190	-0.391	-0.169	-0.046	-0.024	0.053	0.037	0.006	0.006
VAL	0.275	0.033	-0.263	-0.062	0.130	0.050	0.422	0.182	0.421	0.219	-0.462	-0.324	-0.199	-0.191
MET	-0.611	-0.072	0.553	0.131	-0.074	-0.028	0.254	0.110	0.075	0.039	-0.326	-0.229	0.058	0.056
LYS	0.431	0.051	0.476	0.113	0.473	0.181	-0.244	-0.105	0.191	0.099	-0.125	-0.088	-0.018	-0.018
LEU	0.466	0.055	0.460	0.109	0.440	0.168	-0.283	-0.122	0.014	0.008	0.121	0.085	-0.169	-0.162
PHE	0.569	0.067	0.140	0.033	0.316	0.121	-0.278	-0.120	-0.308	-0.161	-0.151	-0.106	0.247	0.238

Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province, China

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材料Material	F₁	F₂	F₃	F₄	F₅	F₆	F₇	F	排名Ranking
GT-AS-70	-10.92	11.29	11.71	21.37	12.80	9.19	-25.68	2.14	1
GT-AS-83	-12.92	12.07	12.45	22.52	14.00	11.22	-29.21	1.80	2
GT-AS-79	-13.76	11.81	14.09	20.16	15.94	10.56	-29.70	1.47	3
GT-AS-81	-14.44	11.89	14.53	20.50	13.87	13.70	-28.50	1.40	4
GT-AS-62	-10.79	8.99	11.66	18.55	12.66	9.39	-27.38	1.36	5
HJY-F1-73	-10.52	8.72	11.75	16.15	12.57	9.08	-24.86	1.26	6
E-38-7	-11.50	9.76	11.38	16.07	12.00	11.05	-23.81	1.16	7
GT-AS-57	-13.87	11.36	14.16	17.08	13.55	12.23	-26.38	1.08	8
GT-GY-05	-14.31	12.40	13.13	18.39	14.25	11.73	-28.10	1.08	9
GT-AS-72	-14.26	10.11	15.18	19.76	14.70	11.36	-28.40	1.05	10
HJY-F1-107	-15.81	13.12	11.99	18.08	13.75	19.37	-31.87	0.75	11
HJY-F1-102	-18.03	11.32	19.85	19.67	17.72	13.97	-30.22	0.73	12
GT-AS-82	-16.17	10.77	16.72	18.16	16.02	11.11	-26.39	0.65	13
HJY-F1-76	-14.40	10.43	13.88	16.98	14.74	10.56	-26.67	0.64	14
GT-AS-19	-19.52	13.98	17.73	20.40	17.07	14.80	-29.52	0.52	15
HJY-F1-55	-16.55	11.19	15.43	19.06	14.85	12.53	-26.89	0.49	16
GZL	-17.93	14.43	14.58	18.95	14.28	13.93	-27.61	0.48	17
GT-AS-77	-15.62	10.49	15.50	16.40	14.58	11.46	-27.80	0.31	18
GT-DZ-06	-17.12	10.70	16.24	18.47	14.19	16.10	-29.54	0.26	19
HJY-F1-23	-16.00	10.72	15.55	15.73	14.46	14.96	-30.52	0.23	20
HJY-F1-8	-16.92	11.71	15.58	16.02	14.79	15.78	-30.43	0.19	21
NW2	-18.75	14.12	14.96	18.80	15.06	15.89	-31.81	0.13	22
GT-AS-58	-16.11	11.39	13.60	15.79	14.22	14.52	-29.03	0.11	23
HJY-F1-64	-16.59	10.57	15.78	16.08	14.31	15.82	-30.78	0.07	24
ZN21	-19.73	15.04	15.17	18.44	16.25	15.24	-30.66	0.03	25
QC1	-20.53	15.52	17.22	17.26	15.75	19.72	-34.20	0.02	26
GT-CR-29	-22.10	17.08	10.62	18.33	25.71	21.72	-36.04	-0.05	27
KM-F1-03	-20.52	12.74	19.62	19.36	17.21	14.87	-33.25	-0.15	28
MT0801	-22.26	17.37	14.45	20.25	17.62	13.74	-28.13	-0.24	29
GT-AS-55	-20.90	13.70	17.33	17.87	16.05	15.69	-31.18	-0.49	30
NW1	-25.02	16.50	21.42	20.79	18.04	20.46	-36.43	-0.50	31
YGS0840	-25.60	16.01	21.60	21.89	18.81	20.70	-37.63	-0.65	32
GT-LP-22	-22.88	14.74	18.18	19.40	17.27	17.79	-33.90	-0.67	33
GT-LP-55	-30.71	20.61	22.42	24.97	21.24	24.83	-46.14	-1.23	34
ZK	-28.35	16.50	23.02	18.49	17.78	26.48	-39.44	-1.60	35

生化成分 Biochemical component	不同类群的含量/(mg·g^-1) Content in different clusters /(mg·g^-1)
生化成分 Biochemical component	类群Ⅰ Cluster Ⅰ	类群Ⅱ Cluster Ⅱ	类群Ⅲ Cluster Ⅲ	类群Ⅳ Cluster Ⅳ
TB	12.70±3.07 a	10.66±2.91 ab	9.07±2.82 b	8.34±1.62 b
TP	0.18±0.08 a	0.19±0.06 a	0.11±0.06 b	0.11±0.03 b
CAF	39.44±6.23 a	39.79±3.07 a	30.93±2.70 b	32.32±3.67 b
GA	0.64±0.07 a	0.51±0.16 ab	0.45±0.14 b	0.53±0.15 ab
EGC	13.19±3.92 a	16.87±2.79 a	16.55±3.82 a	12.92±2.63 a
C	0.65±0.12 a	0.58±0.11 a	0.43±0.06 b	0.47±0.08 b
EC	6.37±1.99 a	7.12±0.89 a	6.65±2.46 a	6.93±1.64 a
EGCG	65.82±8.95 a	75.99±15.38 a	53.62±4.89 b	49.37±7.46 b
GCG	7.19±0.35 a	7.59±1.17 a	8.92±6.96 a	7.18±1.14 a
ECG	28.54±8.58 a	24.80±5.56 a	15.09±4.38 b	17.15±5.16 b
TC	121.76±11.71 a	132.95±19.31 a	101.26±11.18 b	94.02±11.41 b
THE	23.44±3.01 b	24.92±6.30 b	27.84±2.67 ab	32.29±6.27 a
ASP	2.21±0.36 ab	1.82±0.67 b	2.24±0.51 ab	2.97±0.87 a
SER	1.41±0.07 a	0.79±0.18 c	1.05±0.13 b	1.39±0.17 a
GLU	4.36±0.92 b	3.41±0.77 b	5.02±0.71 ab	6.48±1.77 a
GLY	0.06±0.03 b	0.07±0.04 ab	0.11±0.06 a	0.08±0.03 ab
HIS	3.73±2.49 b	2.75±1.28 b	3.93±1.32 b	6.63±2.88 a
ARG	4.82±3.93 b	1.81±1.32 c	6.33±1.64 ab	7.67±2.49 a
THR	0.26±0.09 a	0.04±0.03 b	0.09±0.05 b	0.23±0.09 a
ALA	0.21±0.03 b	0.10±0.06 b	0.17±0.08 b	0.40±0.15 a
PRO	1.57±0.27 ab	1.61±0.43 a	0.94±0.25 c	1.11±0.48 bc
CYS	—	—	0.01±0.01 a	0.01±0.01 a
TYR	0.14±0.12 a	0.02±0.02 b	0.05±0.03 b	0.07±0.03 b
VAL	2.03±0.32 b	3.21±2.39 ab	3.69±0.84 a	3.57±0.93 a
MET	0.33±0.21 a	0.24±0.07 ab	0.10±0.07 c	0.14±0.07 bc
LYS	0.15±0.03 a	0.01±0.01 c	0.07±0.06 bc	0.09±0.06 ab
LEU	0.20±0.11 a	0.08±0.05 c	0.13±0.03 bc	0.16±0.05 ab
PHE	0.26±0.06 a	0.17±0.04 b	0.24±0.08 ab	0.26±0.05 a

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