Prokaryotica expression of rabbit Bordetella bronchiseptica major fimbrial subunit gene fimN

›› 2011, Vol. 23 ›› Issue (5): 0-893.

• 动物科学 •

Prokaryotica expression of rabbit Bordetella bronchiseptica major fimbrial subunit gene fimN

ZHANG Chun-tao;LIU Yan;WEI Qiang;XIAO Chen-wen;BAO Guo-lian;*;JI Quan-an

1College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2Institute of Animal Husbandry and Veterinarian, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

Abstract

Abstract: The full length of fimN from Bordetella bronchiseptica in rabbits was amplified with PCR, and it was cloned into pET-28a(+) vector (named pET28a-fimN). The recombinant plasmid was transformed into E. coli BL-21 cells and induced with IPTG.The results of SDS-PAGE showed that the molecular weight of the recombinant protein was about 27 kD. The result of Western blot showed that the recombination protein could be recognized by the specific antibody against Bb, indicating that the protein could be used as antigen.

Key words: Bordetella bronchiseptica, fimN, expression, purification

ZHANG Chun-tao;LIU Yan;WEI Qiang;XIAO Chen-wen;BAO Guo-lian;*;JI Quan-an. Prokaryotica expression of rabbit Bordetella bronchiseptica major fimbrial subunit gene fimN[J]. , 2011, 23(5): 0-893.

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Prokaryotica expression of rabbit Bordetella bronchiseptica major fimbrial subunit gene fimN

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