A rapid and precise SSRbased procedure for bottle gourd seed quality survey

›› 2012, Vol. 24 ›› Issue (4): 0-581.

• 论文 •

A rapid and precise SSRbased procedure for bottle gourd seed quality survey

LU Zhong-fu;XU Pei;WU Xiao-hua;WANG Bao-geng;WANG Sha;LIU Yong-hua;LI Guo-jing*

Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

Abstract

Abstract: Current application of large number of commercial bottle gourd cultivars with very high genetic similarity has caused many disputes on seed quality. To provide a rapid, precise and reliable method for bottle gourd seed purity assay, we developed a set of SSR markers based on DNA sequences obtained from highthroughput sequencing. Eight of these markers were defined as diagnostic markers by genotyping a collection of 44 bottle gourd genotypes. Based on this, a rapid and precise SSRbased procedure as well as standard DNA fingerprints for bottle gourd seed quality survey was established, which will be beneficial for the industrialization of bottle gourd seeds.

Key words: bottle gourd, SSR, purity survey, genetic fingerprinting

LU Zhong-fu;XU Pei;WU Xiao-hua;WANG Bao-geng;WANG Sha;LIU Yong-hua;LI Guo-jing*. A rapid and precise SSRbased procedure for bottle gourd seed quality survey[J]. , 2012, 24(4): 0-581.

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A rapid and precise SSRbased procedure for bottle gourd seed quality survey

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