Studies on protoplast formation and regeneration of Riemerella anatipestifer strain

›› 2009, Vol. 21 ›› Issue (4): 0-315.

• 论文 •

Studies on protoplast formation and regeneration of Riemerella anatipestifer strain

WANG Chun-ping;;WEI Qiang;CUI Yan-shun;*;BAO Guo-lian;*;LIU Yan;SHAO Ze-Xiang;

1 College of Animal Science and Veterinary Medicine of Shandong Agricultrual University，Tai'an Shandong 271018，China；2 Institute of Animal Husbandry and Veterinary Science，Zhejiang Academy of Agricultural Sciences，Hangzhou Zhejiang 310021，China

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-25 Published:2009-07-25

Abstract

Abstract: Using Riemerella anatipestifer TXRA1(Chl r，Ery s)strain with drug resistance label as the parental strain，protoplasts were pfe with lysozyme and EDTA at the presence of DNase I，then the protoplasts were regenerated on hypertonic
medium．Conditions of protoplast formation and regeneration were optimized．indicating that the optimum Riemerella anatipestifer TXRA1(Chl r，Ery s)added with 0．1 g／L lysozyme and reacted for 20 min and then EDTA Was added slowly to a final concentration of O．O1 mol/L and reacted for another 45 min, in which the protoplast formation rate was92％ and the regeneration rate was 38．77％．

Key words: Riemerella anatipestifer, protoplast, formation, regeneration

WANG Chun-ping;WEI Qiang;CUI Yan-shun;*;BAO Guo-lian;*;LIU Yan;SHAO Ze-Xiang;. Studies on protoplast formation and regeneration of Riemerella anatipestifer strain[J]. , 2009, 21(4): 0-315.

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Studies on protoplast formation and regeneration of Riemerella anatipestifer strain

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