Abstract: Thuidium cymbifolium gametophyte was cultured as experimental material. The effects of zeolite medium on the growth index of Thuidium cymbifolium gametophyte， such as the primary branch length and primary branch number were investigated. The results showed that the best disinfection method was 02% NaClO for 60 s， and the contamination rate， mortality rate on zeolite medium was 0， while the survival rate was 100% after 7 d. It was affected by the zeolite concentration， pH value of medium， agar and sucrose concentration to promote the growth of Thuidium cymbifolium gametophyte on zeolite medium. The optimal condition for the growth of Thuidium cymbifolium gametophyte on zeolite medium was shown as follows: zeolite concentration 20%， pH value 75， agar concentration 10%. The primary branch length was 344 cm， and the number of primary branches was up to 270 after 30 d under that condition．

Key words: Thuidium cymbifolium, gametophyte, zeolite medium, growth, pH value, sucrose, agar